Search results for "microRNA."
showing 10 items of 574 documents
A MiR-142-3p/EGR2 Feedback Circuitry In Human CSF-1 Driven Differentiation of Monocytes Into Macrophages
2011
Abstract Abstract 2366 Colony-stimulating factor-1 (CSF-1 or M-CSF) triggers the differentiation of human peripheral blood monocytes into macrophages through and integrated cytokine/transcription factors circuitry. Using microarray profiling to explore the role of microRNAs (miRNAs) in this molecular circuitry, we identified the down-regulation of miR-142-3p in human macrophages obtained from CSF-1-treated monocytes. We show that miR-142-3p is a repressor of the transcription factor EGR2 (Early Growth Response 2) through direct 3'UTR interactions. Interestingly, EGR2 binds the promoter of the pre-miR-142-3p gene to negatively regulate its expression, identifying a self-regulatory feedback l…
A p53-Dependent Tumor Suppressor Network Is Induced by Selective miR-125a-5p Inhibition in Multiple Myeloma Cells
2014
The analysis of deregulated microRNAs (miRNAs) is emerging as a novel approach to disclose the regulation of tumor suppressor or tumor promoting pathways in tumor cells. Targeting aberrantly expressed miRNAs is therefore a promising strategy for cancer treatment. By miRNA profiling of primary plasma cells from multiple myeloma (MM) patients, we previously reported increased miR-125a-5p levels associated to specific molecular subgroups. On these premises, we aimed at investigating the biological effects triggered by miR-125a-5p modulation in MM cells. Expression of p53 pathway-related genes was down-regulated in MM cells transfected with miR-125a-5p mimics. Luciferase reporter assays confirm…
Role of microRNAs shuttled by exosomes in the crosstalk between chronic myelogenous leukemia and endothelial cells
2013
Ascending aorta phenotypic and genotypic changes in bicuspid aortic valve disease
2018
Bicuspid aortic valve (BAV) with left-right (L-R), right-non coronary (R-NC) and left-non coronary (L-NC) cusp fusion represents distinct pathological entities and the rate of aortic enlargement varies according to the pattern of cusps fusion (1). Here, we investigated the histological features of aneurysms associated to different BAV phenotypes and we looked for specific microRNAs (miRNA) as biomarkers of medial degeneration severity. Aortic specimens and blood were obtained from BAV patients treated surgically for the repair of thoracic aortic aneurysm and were divided into two groups: low grade medial degeneration (LGMD, n=10); high grade medial degeneration (HGMD, n=10). A control group…
MicroRNAs expression in c-Kit+/Sca-1+/MDR-1+ cardiac stem cells in three dimensional cultures
2011
Endoplasmic Reticulum Stress and miRNA Impairment in Aging and Age-Related Diseases
2021
Aging is a physiological process defined by decreased cellular and tissue functions. Reduced capacity of protein degradation is one of the important hallmarks of aging that may lead to misfolded protein accumulation and progressive loss of function in organ systems. Recognition of unfolded/misfolded protein aggregates via endoplasmic reticulum (ER) stress sensors activates an adaptive mechanism, the unfolded protein response (UPR). The initial step of UPR is defined by chaperone enhancement, ribosomal translation suppression, and misfolded protein degradation, while prolonged ER stress triggers apoptosis. MicroRNAs (miRNAs) are non-coding RNAs affecting various signaling pathways through de…
FUNCTIONAL GENOMIC APPROACHES TO ELUCIDATE BIOTIC AND ABIOTIC STRESS RESPONSES IN PLANTS
Análisis del perfil de expresión de microRNAs en tejido embrionario sano y ectópico
2016
La placentación es un proceso fundamental en el éxito de la reproducción, sometido a una estrecha regulación donde el trofoblasto juega una función clave. Este proceso se realiza adecuadamente gracias a la relación estrictamente regulada entre los tejidos materno-fetales, donde intervienen multitud de moléculas y receptores; por tanto es necesario un correcto desarrollo de dicho proceso para asegurar una gestación a término saludable, y la invasión anómala del trofoblasto conducirá a un gran abanico de complicaciones, entre ellas el embarazo ectópico. El proceso de placentación está alterado en las gestaciones ectópicas, produciéndose la implantación fuera de la cavidad endometrial. Las alt…
miRNAs in the metaplastic transformation of the esophagus: something in the way toward carcinogenesis.
Background: Barrett's esophagus (BE) is a precancerous lesion that predisposes to development of esophageal adenocarcinoma (EAC) and it is associated with gastroesophageal reflux disease. Genetic changes involved in the metaplastic progression from squamous esophageal mucosa toward Barrett’s metaplasia and adenocarcinoma are almost unknown and there is an increasing interest in the developing of more objective biomarkers that can improve risk prediction. Several evidences suggest that some miRNAs are differentially expressed in the BE and EAC. Among them, miR-143, miR-145, miR-194, miR-203, miR-205, miR-215 appear to have a key role in the metaplasia and in neoplastic progression. Aim: The …