Search results for "microRNA"
showing 10 items of 577 documents
MicroRNA-29b-1 impairs in vitro cell proliferation, self‑renewal and chemoresistance of human osteosarcoma 3AB-OS cancer stem cells
2014
Osteosarcoma (OS) is the most common type of bone cancer, with a peak incidence in the early childhood. Emerging evidence suggests that treatments targeting cancer stem cells (CSCs) within a tumor can halt cancer and improve patient survival. MicroRNAs (miRNAs) have been implicated in the maintenance of the CSC phenotype, thus, identification of CSC-related miRNAs would provide information for a better understanding of CSCs. Downregulation of miRNA-29 family members (miR-29a/b/c; miR‑29s) was observed in human OS, however, little is known about the functions of miR-29s in human OS CSCs. Previously, during the characterization of 3AB-OS cells, a CSC line selected from human OS MG63 cells, we…
Genetic and Molecular Characterization of The Human Osteosarcoma 3AB-OS Cancer Stem Cell Line: A Possible Model For Studying Osteosarcoma Origin and …
2013
Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical to halt cancer and improve patient survival. Osteosarcoma is an aggressive tumor affecting adolescents, for which there is no second-line chemotherapy. Uncovering new molecular mechanisms underlying the development of osteosarcoma and origin of CSCs is crucial to identify new possible therapeutic strategies. Here, we aimed to characterize genetically and molecularly the human osteosarcoma 3AB-OS CSC line, previously selected from MG63 cells and which proved to have both in vitro and in vivo features of CSCs. Classic cytogenetic studies demonstrated that 3AB-OS cells have hypertriploid karyotype wit…
Expresión de microRNAs en la zonas de transición epitelio mesenquimal en el carcinoma colorectal como factor inmunomodulador y pronóstico. Estudio cl…
2018
El subgrupo mesenquimal (CMS4) de la clasificación molecular de consenso de cáncer colorectal es el tipo más agresivo y de peor pronóstico, y se caracteriza por la sobre expresión de genes implicados en el mecanismo de transición epitelio mesenquimal (TEM). La desdiferenciación tumoral focal (DTF) es el reflejo histológico del proceso de TEM. En base a estos conocimientos nos planteamos explorar la influencia de la DTF en la expresión de genes y microRNAs implicados en el proceso de TEM y evaluar su utilidad como factores predictivos de supervivencia. Analizamos de forma retrospectiva el grado de DTF en una serie de 125 especímenes de colectomía en todos los estadios clínicos. Evaluamos la …
Prognostic Impact of let-7e MicroRNA and Its Target Genes in Localized High-Risk Intestinal GIST: A Spanish Group for Research on Sarcoma (GEIS) Study
2020
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level, and they have been described as being associated with tumor prognosis. Here, miRNA profiling was planned to explore new molecular prognostic biomarkers in localized intestinal high-risk GIST. Paraffin tumor blocks of 14 and 86 patients were used in the discovery and expansion sets, respectively. GeneChip miRNA v3.0 was employed to identify the miRNAs differentially expressed between relapsed and non-relapsed patient samples, which were validated in the expansion set, by qRT-PCR. RT2 Profiler PCR Array was used for the screening of let-7e targets. Expression levels were co…
The Status of EGFR Modulates the Effect of miRNA-200c on ZEB1 Expression and Cell Migration in Glioblastoma Cells
2020
Migration of glioblastoma cells into surrounding tissue is one of the main features that makes this tumor incurable. We evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns in 30 cases of primary glioblastoma. From the 64 miRNAs that showed differential expression between tumors with a high level of EGFR amplification and tumors without EGFR amplification, 40% were related with cell migration, being miR-200c the most differentially expressed between these two groups. We investigated the effect of miR-200c on ZEB1 expression and cell migration in an in vitro transfection model with a miR-200c mimic, a miR-200c inhibitor and siRNA targeting E…
Epigenetics, oxidative states and diabetes
2020
Abstract Although controlling a wide range of physiological cell functions, redox states unbalance during inflammation toward oxidative stress. Specific regions of our genome are extremely sensitive to both oxygen and reactive oxygen species, therefore modulating gene transcription in response to this specific epigenetic effect. Additional epigenetic mechanisms may include the alteration of the methylation states of DNA, protein and lipid nitration or modulation of specific microRNAs. A crucial role in finely tuning redox states is also played by mitochondria, where oxidative phosphorylation is epigenetically controlled. Diabetes, the most considerable “epigenetic” clinical disorder, exacer…
Epigenetic involvement in Hutchinson-Gilford progeria syndrome: a mini-review.
2013
Hutchinson-Gilford progeria syndrome (HGPS) is a rare human genetic disease that leads to a severe premature ageing phenotype, caused by mutations in the <i>LMNA</i> gene. The <i>LMNA</i> gene codes for lamin-A and lamin-C proteins, which are structural components of the nuclear lamina. HGPS is usually caused by a de novo <i>C1824T</i> mutation that leads to the accumulation of a dominant negative form of lamin-A called progerin. Progerin also accumulates physiologically in normal ageing cells as a rare splicing form of lamin-A transcripts. From this perspective, HGPS cells seem to be good candidates for the study of the physiological mechanisms of ageing…
The Copper-microRNA Pathway Is Integrated with Developmental and Environmental Stress Responses in Arabidopsis thaliana
2021
As an essential nutrient, copper (Cu) scarcity causes a decrease in agricultural production. Cu deficiency responses include the induction of several microRNAs, known as Cu-miRNAs, which are responsible for degrading mRNAs from abundant and dispensable cuproproteins to economize copper when scarce. Cu-miRNAs, such as miR398 and miR408 are conserved, as well as the signal transduction pathway to induce them under Cu deficiency. The Arabidopsis thaliana SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) family member SPL7 binds to the cis-regulatory motifs present in the promoter regions of genes expressed under Cu deficiency, including Cu-miRNAs. The expression of several other SPL transcription f…
SAT0390 Microrna Expression Profile in Fibromyalgia Patients: A Pilot Matched Case Control Study
2013
Background Fibromyalgia (FM) is a clinical syndrome characterized by musculoskeletal pain and tenderness. Diagnosis is mainly based on exclusion of other resembling diseases since validated biological tests are still lacking. Identification of disease-related markers will enable clinicians to effectively diagnose FM, follow the progress of the disease, monitor the effects of therapeutic approaches and probably develop preventive programs. MicroRNAs (miRNAs) are pieces of 18-25 nucleotides capable of regulating gene expression. Changes in their expression levels have been linked to environmental responses as well as to pathological processes. Their recent association to some other chronic di…
A novel rationale for targeting FXI: Insights from the hemostatic miRNA targetome for emerging anticoagulant strategies
2018
Abstract The treatment and prevention of thrombosis is currently under a period of rapid change with the replacement of traditional anticoagulant vitamin K antagonists, which impair the biosynthesis of a wide range of blood coagulation factors, with direct oral anticoagulants (DOACs), which specifically target factors FXa or FIIa. Nevertheless therapeutic targeting of blood coagulation is an inherently difficult task as it interferes with the delicate balance of pro- and anticoagulant activities. Although anticoagulants are employed in millions of thrombophilic patients worldwide each year, for a growing population with comorbidities who exhibit an increased risk of bleeding with DOAC treat…