Search results for "microbial"

Assessing the impact on intestinal microbiome and clinical outcomes of antibiotherapy optimisation strategies in haematopoietic stem cell transplant …

2020

GETH (Grupo Español de Trasplante Hematopoyético y Terapia Celular).

Antimicrobial prophylaxis in allogeneic bone marrow transplantation. Guidelines of the Infectious Diseases Working Party (AGIHO) of the German Societ…

2005

Patients undergoing allogeneic stem cell transplantation are at high risk for infection with a variety of pathogens during different phases of the procedure. Bacteria and fungi predominate the first phase until engraftment. During the second phase, from engraftment to about day 100, major infectious problems are caused by fungi and cytomegalovirus. Both pathogens remain important under continued immunosuppression, however, in the late post-transplantation period infections with encapsulated bacteria may become a problem. In this review the Infectious Diseases Working Party of the DGHO gives recommendations for prophylaxis of infections under allogeneic stem cell transplantation with drugs a…

Immune Modulating Effects of NKT Cells in a Physiologically Low Dose Leishmania major Infection Model after αGalCer Analog PBS57 Stimulation

2014

Leishmaniasis is a parasitic infection affecting ∼12 million people worldwide, mostly in developing countries. Treatment options are limited and no effective vaccines exist to date. Natural Killer T (NKT) cells are a conserved innate-like lymphocyte population with immunomodulating effects in various settings. A number of reports state a role of NKT cells in different models of Leishmania infection. Here, we investigated the effect of NKT cells in a physiologically relevant, intradermal low dose infection model. After inoculation of 103 infectious-stage L. major, comparable numbers of skin-immigrating NKT cells in both susceptible BALB/c mice and resistant C57BL/6 mice were noted. Compared …

Genetic features of Multi Drug Resistant (MDR) Klebsiella pneumoniae and its Plasmids

2019

Klebsiella pneumoniae, a major cause of both hospital and community-acquired infections, is listed by the World Health Organization as a critical priority antibiotic- resistant bacterial pathogen. With the appearance of sequencing techniques such as Next-generation Sequencing (NGS), there is the possibility to obtain the whole genome of the bacteria, getting to know all antimicrobial resistance determinants. The purpose of this study has been to apply this new technology to clinical microbiology, in order to characterize the resistome present in carbapenem-resistant K.pneumoniae strains isolated in a tertiary hospital in Valencia, Spain. A total of 234 isolates were prepared for whole-genom…

CH vs. HC—Promiscuous Metal Sponges in Antimicrobial Peptides and Metallophores

2023

Histidine and cysteine residues, with their imidazole and thiol moieties that deprotonate at approximately physiological pH values, are primary binding sites for Zn(II), Ni(II) and Fe(II) ions and are thus ubiquitous both in peptidic metallophores and in antimicrobial peptides that may use nutritional immunity as a way to limit pathogenicity during infection. We focus on metal complex solution equilibria of model sequences encompassing Cys–His and His–Cys motifs, showing that the position of histidine and cysteine residues in the sequence has a crucial impact on its coordination properties. CH and HC motifs occur as many as 411 times in the antimicrobial peptide database, while …

Profiling microbial communities in hyperalkaline waters of the Kizildag ophiolite complex (Turkey).

2016

It has recently been hypothesized that life on Earth could have been originated in hyperalkaline waters related to serpentinization of ophiolitic rocks, despite their extreme conditions (high pH and very low levels of nutrients). Five hyperalkaline springs of the Kizildag ophiolite complex (Turkey) were characterized. The dominant gases are either H2, CH4 or N2 . Bacterial diversity, analysed by RISA (Ribosomal Intergenic Spacer Analysis) revealed different profles for each spring. Sequencing of excised DNA bands allowed to identify the presence of Bacillus, Ralstonia, Pseudoalteromonas., Ureibacillus, Alicycliphilus, Anaerococcus. 16S ribosomal DNA sequencing by Illumina is in progress. Th…

Novel marine invertebrate-derived antimicrobial peptides against staphylococcal biofilm

2009

The role of biotic and abiotic processes in the origin of Messinian evaporate-associated carbonates (Southern Italy)

2013

Biotechnologies for identification of microorganisrns colonizing aerosol and surfaces of works of art in indoor environrnents

2012

Biological deterioration of organic and inorganic objects is a compIex process involving a relevant number of microbi al species. Particularly. fungi é1nd bacteria, wide spread in biosphere environments, are the main microorganisms related to the biodeterioration o[ cultural property. Moreover, complex microbial communities can relcase, in indoor environments, metabolic products or other airborne particles that may be detrimenral for human health, In order lo identify the components of microbial populations colonizing surfaces of works o[ art and/ or dispersed in thc aerosol of confìned/semi-confined environments, a molecular approach was laken. Samples were collected by non-destructive tec…

Effect of seasonality on physico-chemical and microbiological properties of nicastrese milk and artisanal cheese

2021

ABSTRACT Nicastrese is an indigenous Italian goat breed reared in the Calabria region under semi-extensive practices. From January to June, the milk yield of 400 multiparous Nicastrese goats was evaluated. In addition, tank milk and ripened cheese samples were subjected to physico-chemical and microbiological analyses with the aim to assess the effect of the seasonality on quality parameters. The volatile organic compounds profile of the ripened Nicastrese cheese samples was evaluated. Results showed that the seasonality had a significant effect on milk fat, lactose, urea, citric acid contents and acidity. Microbiological analysis of tank milk samples revealed variability among the differen…