Search results for "microbiota"
showing 10 items of 500 documents
Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets.
2014
The routine use of chemotherapy to control bacterial diseases in aquatic populations has resulted in the development and spread of antibiotic resistance. The inclusion of immunostimulants in fish diets (functional diets) is one of the main strategies to solve this threat. This study aimed to analyse the intestinal microbiota of cultured European sea bass (Dicentrarchus labrax) fed two functional diets applying pyrosequencing of PCR-amplified 16S rRNA gene. Quality-filtered reads were assigned to family and genus taxonomic levels using the Ribosomal Database Project classifier. The autochthonous intestinal microbiota of sea bass consisted of two dominant bacterial genera: Dysgonomonas (Bacte…
Dietary polyphenols as antidiabetic agents: Advances and opportunities
2020
Abstract Dietary polyphenols have been widely investigated as antidiabetic agents in cell, animals, human study, and clinical trial. The number of publication (Indexed by Web of Science) on “polyphenols and diabetes” significantly increased since 2010. This review highlights the advances and opportunities of dietary polyphenols as antidiabetic agents. Dietary polyphenols prevent and manage Type 2 diabetes mellitus via the insulin‐dependent approaches, for instance, protection of pancreatic islet β‐cell, reduction of β‐cell apoptosis, promotion of β‐cell proliferation, attenuation of oxidative stress, activation of insulin signaling, and stimulation of pancreas to secrete insulin, as well as…
Metagenomic Analysis of Milk of Healthy and Mastitis-Suffering Women.
2015
Background: Some studies have been conducted to assess the composition of the bacterial communities inhabiting human milk, but they did not evaluate the presence of other microorganisms, such as fungi, archaea, protozoa, or viruses. Objective: This study aimed to compare the metagenome of human milk samples provided by healthy and mastitis-suffering women. Methods: DNA was isolated from human milk samples collected from 10 healthy women and 10 women with symptoms of lactational mastitis. Shotgun libraries from total extracted DNA were constructed and the libraries were sequenced by 454 pyrosequencing. Results: The amount of human DNA sequences was ≥ 90% in all the samples. Among the bacteri…
Gut microbiota changes in rainbow trout, Oncorhynchus mykiss (Walbaum), during organic acid feed supplementation and Yersinia ruckeri infection
2013
Gut microbiota (GM) composition of vertebrates are considered to influence health parameters including immunological parameters in the host but knowledge of these issues in fish are sparse. We have performed a 45 day experimental study elucidating effects of dietary supplementation of organic acids (Mera Cid) and immunostimulants on the GM composition of rainbow trout Oncorhynchus mykiss (Walbaum) and investigated its association with blood parameters and disease resistance (against enteric red mouth disease (ERM) caused the bacterium Yersinia ruckeri). A total of 640 fish received either 1) basic fish feed without organic acids or immunostimulants, 2) fish feed with immunostimulants (β6glu…
Interaction of dietary polyphenols and gut microbiota: Microbial metabolism of polyphenols, influence on the gut microbiota, and implications on host…
2020
Abstract The human gastrointestinal tract is inhabited by a vast number of microorganisms that are called as the microbiota. Each individual harbors a unique gut microbial composition, this composition evolves throughout the host's lifetime and it is easily affected by internal or external changes. It has been shown that gut microbiota plays a crucial role in host's health and as this complex community has the ability to interact with each other and with the host's immune system, the presence or absence of some major species can affect the homeostasis. Diet can be considered as one of the pivotal factors in modulating the functionality, integrity, and composition of the gut microbiota as th…
DIVERSITÀ BATTERICA DI SUOLI MEDITERRANEI SOGGETTI A DESERTIFICAZIONE
2021
Siccità e fenomeni meteorologici estremi contribuiscono al degrado dei suoli mediterranei semi-aridi causando erosione, compattazione, salinizzazione, riduzione della sostanza organica e perdita di biodiversità, con conseguenze negative per l’agricoltura e l’ambiente. Gli effetti dei cambiamenti climatici in atto sono esacerbati da pratiche agronomiche insostenibili e pascolo incontrollato che concorrono alla desertificazione dei suoli. E’ fondamentale monitorare la qualità dei suoli semi-aridi per prevenirne il degrado irreversibile. Tale monitoraggio viene usualmente condotto mediante i tradizionali indicatori chimico-fisici di qualità del suolo. Ancora raramente vengono utilizzati indica…
Desarrollo de Streptococcus dentisani sp. nov, como probiótico para la salud bucodental
2018
Las enfermedades orales como la caries se encuentran entre las más prevalentes en todo el mundo y se desarrollan como consecuencia de una disbiosis microbiana. Probablemente por esta naturaleza polimicrobiana y altamente variable, las aproximaciones de inmunización no son efectivas, y el uso de pre y probióticos que restablezcan la homeostasis han sido propuestas como alternativas prometedoras. Durante años se han probado varias cepas bacterianas como posibles organismos promotores de la salud oral, pero generalmente son especies aisladas de nichos distintos al sitio donde deben ejercer su acción probiótica final, típicamente de muestras fecales. Nuestra hipótesis defiende que los comensale…
Life under the sun: microbial ecology and applications of the solar panel microbiota
2021
El mundo procariota es vasto y diverso, y aún queda una gran fracción por explorar. La diversidad microbiana es el resultado de la evolución y la adaptación. Esta diversidad nos proporciona un increíble arsenal de herramientas únicas y útiles que se pueden utilizar en una amplia gama de aplicaciones industriales y farmacéuticas. La búsqueda de estas herramientas biológicas es lo que conocemos como bioprospección y, teniendo en cuenta que hasta la fecha sólo se ha explorado una fracción de la diversidad microbiana global, el número de cepas, herramientas genéticas o metabolitos con aplicaciones en biotecnología o en biomedicina que aún quedan por descubrir es abrumador. Esto abre una gran op…
The Microbiota Is Not an Organ: Introducing the Muco-Microbiotic Layer as a Novel Morphofunctional Structure
2022
In this paper, we want to refute the notion that the microbiota should be considered an organ, given that an organ comprises tissue of similar or different embryological origin, while the microbiota is a pool of different microbial species originating individually from single replications and not from a common ancestral cellular element. Hence, we would like to propose a new morphological interpretation of its nature, based on the comprehensive context in which these microbes live: a muco-microbiotic layer of hollow organs, such as the airways and the bowel. The above concept should represent not only a new terminological annotation but also a more accurate portrayal of the physiology and p…
Omega-3 PUFAs and gut microbiota: A preventive approach for colorectal cancer
2021
The influence of diet on the composition of the intestinal microbiota and related pathologies has been known for some time. Some classes of nutrients, such as fatty acids belonging to the omega 3 series, have particular effects on the bacteria that make up the intestinal microbiota. ω-3 PUFAs affect the gut microbiota in three different ways: by modulating the type and abundance of intestinal bacteria, by regulate SCFAs levels, and by alter the levels of proinflammatory mediators. Through these modalities, ω-3 PUFAs could be useful for the prevention of intestinal diseases such as Colorectal Cancer (CRC). The ability of ω-3 PUFAs to modulate the intestinal inflammatory response, to preserve…