Search results for "microenvironment."

showing 3 items of 363 documents

Single-cell analysis of population context advances RNAi screening at multiple levels

2012

Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…

toImage ProcessingDruggabilityGenomeImage analysis0302 clinical medicineComputer-AssistedSX00 SystemsX.ch2604 Applied MathematicsSingle-cell analysisRNA interferenceModels2400 General Immunology and MicrobiologyImage Processing Computer-AssistedViralRNA Small Interfering0303 health scienceseducation.field_of_studyApplied MathematicsSystems BiologyGenomics10124 Institute of Molecular Life SciencesCell biologycell variabilityComputational Theory and MathematicsCellular MicroenvironmentVirus DiseasesVirusesRNA ViralRNA InterferenceSingle-Cell AnalysisGeneral Agricultural and Biological SciencesInformation SystemsSystems biologyVirus infectionPopulationContext (language use)Genomics1100 General Agricultural and Biological SciencesBiologySmall InterferingModels BiologicalGeneral Biochemistry Genetics and Molecular BiologySX08 LipidX03 medical and health sciencesViral ProteinsCell-to-cell variability; Image analysis; Population context; RNAi; Virus infection1300 General Biochemistry Genetics and Molecular BiologyHumansComputer Simulationeducation030304 developmental biologyGeneral Immunology and MicrobiologyCell-to-cell variabilityReproducibility of ResultsBayes TheoremcellBiologicalPopulation contextRNAi570 Life sciences; biologyRNA030217 neurology & neurosurgeryHeLa CellsMolecular Systems Biology
researchProduct

Mast cells as protectors of health.

2019

Mast cells (MCs), which are well known for their effector functions in T(H)2-skewed allergic and also autoimmune inflammation, have become increasingly acknowledged for their role in protection of health. It is now clear that they are also key modulators of immune responses at interface organs, such as the skin or gut. MCs can prime tissues for adequate inflammatory responses and cooperate with dendritic cells in T-cell activation. They also regulate harmful immune responses in trauma and help to successfully orchestrate pregnancy. This review focuses on the beneficial effects of MCs on tissue homeostasis and elimination of toxins or venoms. MCs can enhance pathogen clearance in many bacter…

tumorImmunologyvenomTryptaseMast cell; innate immunity; infection; mast cell protease; tumor; pregnancy; venom; toxin; central nervous system traumaInfectionsCell DegranulationMast Cell ; Innate Immunity ; Infection ; Mast Cell Protease ; Tumor ; Pregnancy ; Venom ; Toxin ; Central Nervous System TraumaImmune systemCathelicidinsPregnancymedicineImmunology and AllergyAnimalsHomeostasisHumansEmbryo ImplantationMast CellsCNS traumatoxininnate immunityTissue homeostasismast cell proteaseToll-like receptorTumor microenvironmentInnate immune systembiologybusiness.industryDegranulationMast cellhumanitiesImmunity InnateToll-Like Receptor 2infectionddc:medicine.anatomical_structureImmunologybiology.proteinFemalepregnancybusinessmast cell
researchProduct

Metabolic Changes in Tumor Microenvironment: How Could They Affect γδ T Cells Functions?

2021

The metabolic changes that occur in tumor microenvironment (TME) can influence not only the biological activity of tumor cells, which become more aggressive and auto sustained, but also the immune response against tumor cells, either producing ineffective responses or polarizing the response toward protumor activity. γδ T cells are a subset of T cells characterized by a plasticity that confers them the ability to differentiate towards different cell subsets according to the microenvironment conditions. On this basis, we here review the more recent studies focused on altered tumor metabolism and γδ T cells, considering their already known antitumor role and the possibility of manipulating th…

tumoral metabolismQH301-705.5T-LymphocytesPopulationReviewMajor histocompatibility complexγδ T cellsImmune systemAntigens NeoplasmIn vivomedicineAnimalsHumanstumor microenvironmentBiology (General)educationtumoral metabolism; ?? T cells; tumor microenvironmentClinical Trials as Topiceducation.field_of_studyTumor microenvironmentbiologyReceptors Antigen T-Cell gamma-deltaBiological activityGeneral MedicineHypoxia (medical)Lipid MetabolismIn vitroCell biologybiology.proteinmedicine.symptom
researchProduct