Search results for "microfluidics"
showing 10 items of 121 documents
M14_Microfluidics_for_CNT
2018
The CNT dispersion with low SDBS content allows stable droplet formation but leads to fast formation of CNT plugs in the hydrophobic channels. The selective channel surface treatment allows long-term stable functionality of the microfluidic system.
Engineered membranes for residual cell trapping on microfluidic blood plasma separation systems. A comparison between porous and nanofibrous membranes
2020
AbstractBlood-based clinical diagnostics require challenging limit-of-detection for low abundance, circulating molecules in plasma. Micro-scale blood plasma separation (BPS) has achieved remarkable results in terms of plasma yield or purity, but rarely achieving both at the same time. Here, we proposed the first use of electrospun polylactic-acid (PLA) membranes as filters to remove residual cell population from continuous hydrodynamic-BPS devices. The membranes hydrophilicity was improved by adopting a wet chemistry approach via surface aminolysis as demonstrated through Fourier Transform Infrared Spectroscopy and Water Contact Angle analysis. The usability of PLA-membranes was assessed th…
M11_Microfluidics_for_CNT
2018
The subtle solubility of water in n-decane causes leaking of the droplet content. If the n-decane flows by static droplets, it carries the water slowly away up to the total disappearance of the droplets.
MICROFLUIDIC PROCEDURE FOR FABRICATION OF HYALURONIC ACID BASED BIOMATERIALS
2017
DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen
2020
Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a si…
Flow Behavior of Chain and Star Polymers and Their Mixtures
2018
Star-shaped polymers show a continuous change of properties from flexible linear chains to soft colloids, as the number of arms is increased. To investigate the effect of macromolecular architecture on the flow properties, we employ computer simulations of single chain and star polymers as well as of their mixtures under Poiseuille flow. Hydrodynamic interactions are incorporated through the multi-particle collision dynamics (MPCD) technique, while a bead-spring model is used to describe the polymers. For the ultradilute systems at rest, the polymers are distributed uniformly in the slit channel, with a weak dependence on their number of arms. Once flow is applied, however, we find that the…
Fabrication techniques for developing a functional microfluidic glass device suitable for detection in optical spectroscopy system
2012
Microfluidic devices offer the chance to manipulate and analyze fluids including bioassays and chemical reactions. In this study, a method to develop a microfluidic analysis system is proposed for detection of nanotubes by a Raman acquisition setup. Microchannels where fabricated in sodalime glass substrate by MeV ion beam lithography or electron beam lithography and wet etching. Fusion bonding (550 °C) was used to seal the microchannels. As a result a prototype microfluidic device with 1.6 µm deep channel that exhibit efficient sealing and suitable channel geometry was obtained. The microfluidic device was tested in a Raman spectroscopy detection system and the collected spectra showed the…
Conic optical fiber probe for generation and characterization of microbubbles in liquids
2021
A novel optical fiber probe has been developed to provide mechanical stability to microbubbles generated in fluids, the tip of the fiber is etched with hydrofluoric acid to pierce a truncated horn that fastens the microbubbles to the fiber tip and prevents misalignment or detachment caused by convection currents, vibrations or shocks in the liquid. Microbubbles are photo-thermally generated on the etched fiber and used as Fabry-Perot cavity sensor. Two methods were used to interrogate the probe: the first one, in the wavelength domain, is suitable for calibration in static or quasi static situations; the second one, in the time domain, can be used in dynamic environments. Experimental resul…
Advances in Nanoliposomes Production for Ferrous Sulfate Delivery
2020
In this study, a continuous bench scale apparatus based on microfluidic fluid dynamic principles was used in the production of ferrous sulfate-nanoliposomes for pharmaceutical/nutraceutical applications, optimizing their formulation with respect to the products already present on the market. After an evaluation of its fluid dynamic nature, the simil-microfluidic (SMF) apparatus was first used to study the effects of the adopted process parameters on vesicles dimensional features by using ultrasonic energy to enhance liposomes homogenization. Subsequently, iron-nanoliposomes were produced at different weight ratios of ferrous sulfate to the total formulation components (0.06, 0.035, 0.02, an…
Development of microfluidics for sorting of carbon nanotubes
2018
Sorting of carbon nanotubes by their chirality is the current bottleneck in the way to their broad employment based on their exceptional electronic and optical properties. Despite the extensive effort, there is no known method, which would result in really pure chirality ensembles. Previously reported sorting protocols result in enrichment rather than in sorting, alter electronic structure, and suffer from low yield. This is mostly due to the statistical approach, where the nanotubes with mixed chiralities are treated as a set. In this thesis, we propose a new sorting technique based on nanotube-by-nanotube compartmelization, characterization, and sorting in a continuously running droplet-b…