Search results for "microscopi"
showing 10 items of 232 documents
Classification par méthodes d’apprentissage supervisé et faiblement superviséd’images multimodales pour l’aide au diagnostic du lentigo malin en derm…
2021
Carried out in collaboration with the Saint-Étienne University Hospital, this work provides additional information to help the skin diagnosis by providing new decision methods on Lentigo Maligna and Lentigo Maligna Melanoma pathologies. To this end, the modalities regularly used in clinical conditions are made available to this work and are orchestrated within a multimodal process. Among image modalities, may be mentioned the clinical photography, the dermatoscopy, and the confocal reflectance microscopy. Initially, the first steps of this manuscript focus on reflectance confocal microscopy as the work in computer diagnostic assistance is relatively underdeveloped, in particular on the dete…
Anti‐laminin auto antibodies in ANCA‐associated vasculitis
2000
Background. Endothelial cell damage occurs during vasculitic processes in vivo. With the alteration of the endothelium, exposure to basement membrane components may occur with induction of humoral immunity. Methods. In the present study, we evaluated the prevalence of antibodies against the basement membrane antigen laminin (LMN) in patients with ANCA-associated systemic vasculitis (AASV), pathologic controls (systemic lupus erythematosus, mixed cryoglobulinaemia, Henoch Schonlein purpura, primary glomerulonephritis) and normal individuals. Results. By ELISA, 21.6% of AASV (16/74) and 10% of pathologic controls (3/30), but only one of the normal controls (2.8%) had these antibodies (P = 0.0…
Nonlocal Second Order Vehicular Traffic Flow Models And Lagrange-Remap Finite Volumes
2011
In this paper a second order vehicular macroscopic model is derived from a microscopic car–following type model and it is analyzed. The source term includes nonlocal anticipation terms. A Finite Volume Lagrange–remap scheme is proposed.
Modelling of interference fits with taking into account surfaces roughness with homogenization technique
2013
International audience; The assembly technique by shrink fit is increasingly used today because it allows for the assembly of two pieces without any intermediary part simply by the tightening effect given by the difference in diameters of the two parts assembled. The definition of assemblies depends on calculation models available in the standard. They make very restrictive assumptions that limit the geometrical defects and the surface finish. It is increasingly common to use a finite element method to better adapt the model to the complex forms of industrial parts. However, the standard is limited with regard to the consideration of roughness which results in a loss of tightening. An easy …
Analyse locale des sensibilités des lectures angulaires, spectroscopiques et ellipsométriques de la Résonance des Plasmons de Surface en vue de la mi…
2006
The fluorescence labelling, used by the fluorescence biochips, is known to change the charge distribution of the labelled molecules and then modify their biological activity. Among label free biosensors, we choose an optical detection, such as spectroscopic ellipsometry and/or surface plasmon resonance (SPR) of the biolayers.These optical techniques are based on the measurement of both thickness and optical index of the adsorbed biolayer. An AFM statistical measurement of the thickness of functionalized lithographed microstructures has been performed in order to determine the thickness of the biolayers.We have shown that the spectroscopic reading of the SPR phase shift is 100 times more sen…
Identification of the contaminants present on the surface of lactose for pharmaceutical use and analysis of the impact of their presence on the inter…
2018
The discovery of the problem will go through training techniques at the nanoscale characterization and chemical analysis, the discovery of the industrial problem in society Armor Proteins, the validation of a pharmaceutical lactose. With the aim to increase our knowledge on powders for food or pharmaceutical use, we propose to study the effect of manufacturing processes, shaping, granulation on the reactivity of powders. The first part is devoted to the effect of purity on interactions-Lactose Lactose and Lactose-API measured / quantified by near-field techniques (AFM, SMM, MSAFM) under ambient atmosphere and under stressful conditions storage. Deriving a second portion of the preceding par…
Exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of …
2015
ABSTRACT Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Pr…
Etude dynamique et structurale de biomolécules par microscopie à force atomique HS-AFM : application à une petite protéine de choc thermique sHsp
2012
The atomic force microscopy (AFM) gives access to the topography of organic and inorganic samplesat the atomic scale. The latest innovations offer the possiblity to understand the sample nano-mechanicalproperties (elasticity, adhesion...). Its feature set allows overcoming the demands of nanotechnology,both in the fields of physics, chemistry and biology.However, understanding biological processes require faster acquisitions for the atomic forcemicroscopy, less than a second per frame. As conventional equipment does not offer the possibility toovercome the constraint of time for dynamical studies, a prototype of high-speed atomic forcemicroscope (HS-AFM) was developed in partnership with Pr…
Localisation et visualisation de transcrits fongiques in situ: Une méthode originale qui combine biologie moléculaire et microscopie confocale
2011
National audience; Les Gloméromycètes, formant la symbiose mycorhizienne à arbuscules, sont des champignons biotrophes obligatoires qui sont intimement associés aux tissus végétaux qu’ils colonisent. Les techniques utilisées pour suivre l’expression des gènes fongiques dans les racines mycorhizées ne permettent pas de définir le profil spatio-temporel de leur activité. Afin de tracer l’activité transcriptionnelle fongique dans les racines mycorhizées, une méthode innovante, basée sur la RT-PCR in situ, a donc été développée. Cette technique permet de localiser, en microscopie optique confocale à balayage laser, des transcrits fongiques, à l’aide d’amorces spécifiques marquées par un fluoroc…
Techniques d'études de la formation des biofilms. Retour d'expérience issu du domaine "agro-alimentaire" et du domaine "environnemental"
2008
No abstract