Search results for "middle"

showing 10 items of 15335 documents

ΔNp63 drives metastasis in breast cancer cells via PI3K/CD44v6 axis

2016

P63 is a transcription factor belonging to the family of p53, essential for the development and differentiation of epithelia. In recent years, it has become clear that altered expression of the different isoforms of this gene can play an important role in carcinogenesis. The p63 gene encodes for two main isoforms known as TA and ΔN p63 with different functions. The role of these different isoforms in sustaining tumor progression and metastatic spreading however has not entirely been clarified. Here we show that breast cancer initiating cells express ΔNp63 isoform that supports a more mesenchymal phenotype associated with a higher tumorigenic and metastatic potential. On the contrary, the ma…

0301 basic medicineGene isoformEpithelial-Mesenchymal TransitionBreast Neoplasmsmedicine.disease_causeMetastasisMicePhosphatidylinositol 3-Kinases03 medical and health sciencesBreast cancerTumor MicroenvironmentmedicineAnimalsHumansmetastasisEpithelial–mesenchymal transitionNeoplasm MetastasisPI3K/AKT/mTOR pathwayAgedAged 80 and overTumor microenvironmentp63breast cancer initiating cellsbusiness.industryMembrane ProteinsCD44v6Middle Agedmedicine.diseasePI3K/AKT pathwayHyaluronan Receptors030104 developmental biologyOncologyDrug Resistance NeoplasmTumor progressionImmunologyCancer researchFemalebreast cancer initiating cellmetastasibusinessCarcinogenesisProto-Oncogene Proteins c-aktSignal TransductionPriority Research Paper
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Cirrhosis risk score of the donor organ predicts early fibrosis progression after liver transplantation.

2019

Background & Aims: Fibrosis progression (FP) after liver transplantation (LT) increases morbidity and mortality. Biomarkers are needed for early prediction of FP. A recipient’s seven-gene cirrhosis risk score (CRS) has been associated with FP, especially in non-transplant cohorts. A broader validation of CRS, including the genotype of the donor-organ and HCV-negative patients is lacking. We therefore analyzed the impact of donor- and recipient-specific genotypes on FP after LT in a large cohort of HCV-positive and -negative patients.Method: Genotyping from liver biopsies (n=201 donors) and peripheral blood (n=442 recipients) was performed. Cirrhosis risk score was correlated with FP at …

0301 basic medicineGenetic MarkersLiver CirrhosisMalemedicine.medical_specialtyCirrhosisTime Factorsmedicine.medical_treatmentLiver transplantationGastroenterologyRisk Assessment03 medical and health sciences0302 clinical medicineFibrosisRisk FactorsInternal medicineGenotypeotorhinolaryngologic diseasesMedicineHumansCumulative incidenceGenetic Predisposition to DiseaseFramingham Risk Scorebusiness.industryHazard ratioGastroenterologyMiddle Agedmedicine.diseaseTissue DonorsLiver Transplantation030104 developmental biologyPhenotypeTreatment OutcomeDisease Progression030211 gastroenterology & hepatologyFemalebusinessRisk assessmentJournal of gastrointestinal and liver diseases : JGLD
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Clinical and molecular characterization of 112 single-center patients with Neurofibromatosis type 1.

2018

Abstract Background The aim of this retrospective study was to define clinical and molecular characteristics of a large sample of neurofibromatosis type 1 (NF1) patients, as well as to evaluate mutational spectrum and genotype-phenotype correlation. NF1 is a relatively common neurogenetic disorder (1:2500–1:3000 individuals). It is caused by mutations of the NF1 gene on chromosome 17ql1.2, with autosomal dominant pattern of inheritance and wide phenotypical variability. Café-au-lait spots (CALs), cutaneous and/or subcutaneous neurofibromas (CNFs/SCNFs), skinfold freckling, skeletal abnormalities, Lisch nodules of the iris and increased risk of learning and intellectual disabilities, as well…

0301 basic medicineGenotype-phenotype correlation; New mutation; NF1 gene; NF1 microdeletion syndrome; Adolescent; Adult; Age Factors; Child; Child Preschool; Cohort Studies; DNA Mutational Analysis; Female; Genes Neurofibromatosis 1; Genetic Association Studies; Genetic Predisposition to Disease; Humans; Italy; Male; Middle Aged; Neurofibromatosis 1; Prevalence; Prognosis; Retrospective Studies; Risk Assessment; Sex Factors; Young Adult; Mutation MissenseMaleGenotype-phenotype correlationDNA Mutational AnalysisDiseaseCohort Studies0302 clinical medicineDNA Mutational AnalysisGenotypePrevalenceMedicineYoung adultChildNew mutationlcsh:RJ1-570Age FactorsMiddle AgedPrognosisItalyNF1 geneChild PreschoolCohortFemaleNF1 microdeletion syndromeCohort studyAdultmedicine.medical_specialtycongenital hereditary and neonatal diseases and abnormalitiesNeurofibromatosis 1AdolescentMutation MissenseRisk Assessment03 medical and health sciencesYoung AdultSex FactorsGenes Neurofibromatosis 1HumansGenetic Predisposition to DiseaseNeurofibromatosisPreschoolGenetic Association StudiesRetrospective Studiesbusiness.industryResearchRetrospective cohort studylcsh:Pediatricsmedicine.diseaseDermatology030104 developmental biologyGenesPediatrics Perinatology and Child HealthMutationMissensebusiness030217 neurology & neurosurgeryItalian journal of pediatrics
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Oxidative stress and exceptional human longevity: Systematic review.

2020

Oxidative stress (OS) has been previously linked to the aging process, as have some diseases and geriatric syndromes as frailty and sarcopenia. The aim of the present study was to perform a systematic review on oxidative stress activity and extreme longevity in humans.We conducted a systematic literature review following the PRISMA guidelines. Observational studies assessing OS-biomarkers and/or antioxidants in long-lived individuals (97 years old or over) comparing them to those of one or more age groups, (at least one of which from comprising elderly subjects) were considered for inclusion. A narrative synthesis was planned. Quality of selected studies was assessed using the Newcastle-Ott…

0301 basic medicineGerontologyAdultAgingmedia_common.quotation_subjectLongevitymedicine.disease_causeBiochemistryAntioxidants03 medical and health sciencesYoung Adult0302 clinical medicinePhysiology (medical)Plasma lipidsmedicineHumansmedia_commonAgedAged 80 and overbusiness.industrySuperoxide DismutaseLongevityMiddle Agedmedicine.diseaseOxidative Stress030104 developmental biologySystematic reviewSarcopeniaHuman longevityExtreme longevity trackingObservational studyLipid Peroxidationbusiness030217 neurology & neurosurgeryOxidative stressFree radical biologymedicine
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Targeting aging with functional food: Pasta with opuntia single-arm pilot study

2018

Interventions to extend life span represent the new perspective in aging investigation. Healthy dietary habits are important modifiable factors that can favor a healthy aging phenotype. Many studies have demonstrated benefits for metabolic syndrome and type 2 diabetes mellitus resulting from the traditional Mediterranean foods. Opuntia Ficus Indica (OFI), widespread in the Mediterranean basin, belongs to the Cactaceae family. It is known for its antioxidant and anti-inflammatory properties. Moreover, products containing extracts from OFI fruits or cladodes have been used to control obesity and other metabolic parameters, such as glycemia and lipid profile. The aim of this study was to analy…

0301 basic medicineGerontologyAdultMaleAgingMediterranean dietLongevityPsychological interventionAnti-Inflammatory AgentsPilot Projects030204 cardiovascular system & hematologyBiologyAntioxidantsOpuntia Ficus Indica03 medical and health sciencesYoung Adult0302 clinical medicineFunctional foodFunctional FoodMediterranean dietDiabetes MellitusHumansLongitudinal StudiesObesityHealthy agingAgedCell ProliferationInflammationMetabolic Syndromeage-related diseaseoxidative streLife spanTraditional medicinePlant ExtractsPerspective (graphical)food and beveragesOpuntiaMiddle AgedHealthy VolunteersOxidative Stress030104 developmental biologyPhenotypenutritionDiabetes Mellitus Type 2Cardiovascular DiseasesDietary SupplementsFemaleGeriatrics and Gerontology
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Motivators, barriers and strategies of weight management: A cross-sectional study among Finnish adults.

2018

Abstract Background Weight management (WM) is an ongoing global challenge. The purpose of this study was to analyze motivators, barriers, and strategies of WM among Finnish adults. Methods Data were collected in the ‘KULUMA’ (Consumers at the Weight Management Market) project among 667 community-dwelling adults in Eastern and Central Finland (Kuopio and Jyvaskyla). The self-reported questionnaire collected background information and responses to motivators, barriers, and strategy items. Principal component analysis (PCA) was used to extract components of motivators, barriers, and strategies of WM, along with K-means clustering to categorize the participants. Results About 55% of the respond…

0301 basic medicineGerontologyBackground informationAdultMaleCross-sectional studybarriersmotivatorspainonhallintaBody Weight Maintenance03 medical and health sciences0302 clinical medicineWeight lossSurveys and QuestionnairesWeight managementWeight LossmedicineCluster AnalysisHumans030212 general & internal medicineLife Styleta515Finlandta415motivaatioMotivationPrincipal Component Analysis030109 nutrition & dieteticsMiddle AgedesteetDietstrategiatweight managementPsychiatry and Mental healthClinical PsychologyCross-Sectional StudiesCategorizationstrategiesklusterianalyysiFemalemedicine.symptomPsychologyPsychosocialFood environmentEating behaviors
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Legume consumption is inversely associated with type 2 diabetes incidence in adults: A prospective assessment from the PREDIMED study

2018

Background & aims: Legumes, a low-energy, nutrient-dense and low glycemic index food, have shown beneficial effects on glycemic control and adiposity. As such, legumes are widely recommended in diabetic diets, even though there is little evidence that their consumption protects against type 2 diabetes. Therefore the aim of the present study was to examine the associations between consumption of total legumes and specific subtypes, and type 2 diabetes risk. We also investigated the effect of theoretically substituting legumes for other protein- or carbohydrate-rich foods. Methods: Prospective assessment of 3349 participants in the PREvención con DIeta MEDiterránea (PREDIMED) study without ty…

0301 basic medicineGerontologyBlood GlucoseMaleLentilsMediterranean dietPREDIMED-study030209 endocrinology & metabolismContext (language use)Type 2 diabetesCritical Care and Intensive Care MedicineLower riskDiet Mediterranean03 medical and health sciences0302 clinical medicineRisk FactorsDiabetes mellitusmedicineHumansProspective StudiesGlycemicAdiposityAgedProportional Hazards Models030109 nutrition & dieteticsNutrition and Dieteticsbusiness.industryFabaceaeType 2 diabetesMiddle Agedmedicine.diseaseLegumesDietGlycemic indexQuartileDiabetes Mellitus Type 2Glycemic IndexFemalebusinessDemographyFollow-Up Studies
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The prevalence of malnutrition according to the new ESPEN definition in four diverse populations

2016

© 2015 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism.Background & aims: Consensus on the definition of malnutrition has not yet been reached. Recently, The European Society for Clinical Nutrition and Metabolism (ESPEN) proposed a consensus definition of malnutrition. The aim of the present study was to describe the prevalence of malnutrition according to the ESPEN definition in four diverse populations. Methods: In total, 349 acutely ill middle-aged patients, 135 geriatric outpatients, 306 healthy old individuals and 179 healthy young individuals were included in the study. Subjects were screened for risk of malnutrition using the SNAQ. The ESPEN definition of maln…

0301 basic medicineGerontologyMalePediatricsNutritional SciencesCritical Care and Intensive Care MedicineThinness/etiologygeriatric patientBody Mass IndexNutritional Sciences/methods0302 clinical medicineunderweightWeight loss/dk/atira/pure/sustainabledevelopmentgoals/zero_hungerPrevalenceMedicine030212 general & internal medicineYoung adultcomparative studypathophysiologyhealth care organizationNutrition and Dieteticsta3141Scientificta3142Middle AgedEuropenutritional assessmentElder Nutritional Physiological PhenomenaoutpatientAcute DiseasePractice Guidelines as TopicBody CompositionFemalemedicine.symptomRisk assessmentSocieties ScientificPredictive validityAdultRiskmedicine.medical_specialtyConsensusgeriatric nutritionNutritional Statusmalnutritionadult; aged; Article; body mass; controlled study; disease predisposition; fat free mass; female; geriatric patient; human; major clinical study; male; malnutrition; middle aged; nutritional assessment; outpatient; population research; predictive validity; prevalence; risk assessment; short nutritional assessment questionnaire; weight reduction; acute disease; body composition; comparative study; consensus; Europe; geriatric assessment; geriatric nutrition; health care organization; malnutrition; nutritional assessment; nutritional science; nutritional status; pathophysiology; practice guideline; procedures; risk; risk assessment; underweight; young adult Acute Disease; Adult; Aged; Body Composition; Body Mass Index; Consensus; Elder Nutritional Physiological Phenomena; Europe; Female; Geriatric Assessment; Humans; Male; Malnutrition; Middle Aged; Nutrition Assessment; Nutritional Sciences; Nutritional Status; Practice Guidelines as Topic; Prevalence; Risk; Risk Assessment; Societies Scientific; Thinness; Young Adult; Body mass index; Definition; Fat free mass index; Malnutrition; PrevalenceRisk AssessmentArticleEurope/epidemiology03 medical and health sciencesYoung AdultThinnessFat free mass indexHumanscontrolled studyhumanproceduresSDG 2 - Zero HungerGeriatric AssessmentAged030109 nutrition & dieteticsbusiness.industrypractice guidelinedisease predispositionDefinitionNUTRITION&DIETETICSmedicine.diseasemajor clinical studybody masspredictive validityMalnutritionNutrition Assessmentfat free massweight reductionshort nutritional assessment questionnairenutritional scienceNutritional sciencebusinessSocietiespopulation researchBody mass indexMalnutrition/diagnosisClinical Nutrition
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Serum metabolomic profiling facilitates the non-invasive identification of metabolic biomarkers associated with the onset and progression of non-smal…

2016

Lung cancer (LC) is responsible for most cancer deaths. One of the main factors contributing to the lethality of this disease is the fact that a large proportion of patients are diagnosed at advanced stages when a clinical intervention is unlikely to succeed. In this study, we evaluated the potential of metabolomics by H-1-NMR to facilitate the identification of accurate and reliable biomarkers to support the early diagnosis and prognosis of non-small cell lung cancer (NSCLC). We found that the metabolic profile of NSCLC patients, compared with healthy individuals, is characterized by statistically significant changes in the concentration of 18 metabolites representing different amino acids…

0301 basic medicineGerontologyOncologyAdultMalemedicine.medical_specialtyLung NeoplasmsProton Magnetic Resonance SpectroscopyDiseaseNSCLC03 medical and health sciences0302 clinical medicineMetabolomicsInternal medicineCarcinoma Non-Small-Cell LungmedicineBiomarkers TumorHumansLung cancerAgedbusiness.industryNon invasivebiomarkersLipid metabolismMiddle Agedmedicine.diseasemetabolomicsPathophysiology030104 developmental biologyMetabolomic profilingOncology030220 oncology & carcinogenesisDisease ProgressionFemaleNon small cellprognosisbusinessResearch Paperearly diagnosis
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Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Bra…

2018

BACKGROUND: Intestinal protozoan parasites are major contributors to the global burden of gastrointestinal disease causing significant socioeconomic consequences. Children living in resource-poor settings with restricted access to water and sanitary services are particularly at risk of these infections. METHODS: A prospective, community-based, cross-sectional survey was conducted in Paraná (southern Brazil) between May 2015 and May 2016. A total of 766 stool samples were individually collected from volunteers (male/female ratio: 0.99; age range: 0-76 years) and used for investigating the presence of intestinal helminth and protozoan species by routine microscopic procedures including the Ka…

0301 basic medicineGiardiasisMaleVeterinary medicineCommunityBlastocystis Infectionsmedicine.disease_causeFeces0302 clinical medicineResidence CharacteristicsSurveys and QuestionnairesPrevalenceProspective StudiesIntestinal Diseases ParasiticChildNematodeeducation.field_of_studyMicroscopySoil-transmitted helminthsMiddle AgedInfectious DiseasesChild PreschoolFemaleAscaris lumbricoidesBrazilHumanAdultGenotypingAdolescent030231 tropical medicinePopulationIntestinal parasiteBiologyDNA Ribosomallcsh:Infectious and parasitic diseases03 medical and health sciencesYoung Adultparasitic diseasesmedicineHelminthHelminthsHumanslcsh:RC109-216ProtozoaeducationAgedIntestinal parasitesBlastocystisMolecular epidemiologyEndolimax nanaResearchInfant NewbornGenetic VariationInfantbiology.organism_classification030104 developmental biologyCross-Sectional StudiesBlastocystisTrichuris trichiuraParasitologyGiardia lamblia
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