Search results for "mikrobit"
showing 6 items of 26 documents
Does Intraspecific Variation in rDNA Copy Number Affect Analysis of Microbial Communities?
2021
Amplicon sequencing of partial regions of the ribosomal RNA loci (rDNA) is widely used to profile microbial communities. However, the rDNA is dynamic and can exhibit substantial interspecific and intraspecific variation in copy number in prokaryotes and, especially, in microbial eukaryotes. As change in rDNA copy number is a common response to environmental change, rDNA copy number is not necessarily a property of a species. Variation in rDNA copy number, especially the capacity for large intraspecific changes driven by external cues, complicates analyses of rDNA amplicon sequence data. We highlight the need to (i) interpret amplicon sequence data in light of possible interspecific and intr…
Arbuscular mycorrhiza, resource availability and belowground interactions between plants and soil microbes
2009
Anne Pietikäinen tutki väitöskirjassaan arbuskelimykorritsasymbioosia ja maanalaisten vuorovaikutusten merkitystä kasvien välisissä suhteissa. Tutkimus valottaa mekanismeja, jotka määräävät kasvillisuuden uusiutumista, erityisesti arktisessa ympäristössä. Lisäksi tutkimustulosten perusteella voidaan ymmärtää kasvillisuuden muuttumista tai säilymistä muuttuvien ympäristötekijöiden paineen alla. Tutkimukset koostuivat ala-arktisilla niityillä Kilpisjärvellä tehdyistä maastokokeista sekä yksinkertaistetuissa systeemeissä toteutetuista kasvihuone- ja kasvukammiokokeista.Maanalainen kilpailu aikuisten kasvien kanssa on yksi tärkeimmistä taimien kasvua heikentävistä tekijöistä. Pietikäinen havait…
Typensidonta ja siihen osallistuva mikrobiyhteisö soiden primäärisukkessiojatkumon rahkasammalissa
2011
Rahkasammalet (Sphagnum spp.) ovat pohjoisten soiden valtalajeja, joiden rakenne ja toiminta vaikuttavat suoekosysteemin sukkessioon. Turpeenmuodostuksen ansiosta pohjoisten seutujen rahkasammalvaltaisten soiden merkitys on suuri myös maailmanlaajuisessa hiilen kierrossa. Sammalten ylläpitämien kosteiden olojen ansiosta soilla elää monimuotoinen aineiden kiertoon osallistuva mikrobiyhteisö. Rahkasammalissa elävät metanotrofit tarjoavat rahkasammalille hiilen lähteen metaaninhapetuksessa (CH4) syntyvästä hiilidioksidista. Metaaninhapetuksen lisäksi metanotrofit voivat sitoa typpeä. Soilla elää myös muita bakteereja, joilla on edellytykset typensidontaan. Rahkasammalet voivat tarjota myös näi…
Multispecies coinfections and presence of antibiotics shape resistance and fitness costs in a pathogenic bacterium
2023
Increasing antimicrobial resistance (AMR) poses a challenge for treatment of bacterial diseases. In real life, bacterial infections are typically embedded within complex multispecies communities and influenced by the environment, which can shape costs and benefits of AMR. However, knowledge of such interactions and their implications for AMR in vivo is limited. To address this knowledge gap, we investigated fitness-related traits of a pathogenic bacterium (Flavobacterium columnare) in its fish host, capturing the effects of bacterial antibiotic resistance, coinfections between bacterial strains and metazoan parasites (fluke Diplostomum pseudospathaceum) and antibiotic exposure. We quantifie…
Three Phages from a Boreal Lake during Ice Cover Infecting Xylophilus, Caulobacter, and Polaromonas Species
2023
Although the important role of microbes in freshwater is well understood, studies on phage–host systems in such environments during ice cover are completely lacking. Here, we describe the isolation and characterization of three new bacteriophages infecting Xylophilus sp., Caudobacter sp., and Polaromonas sp. from freshwater samples taken under the ice cover of Lake Konnevesi, Finland. Lumi, Kuura, and Tiera bacteriophages have tailed icosahedral virions and double-stranded DNA. Lumi is a siphophage with a genome of 80,496 bp, and Kuura and Tiera are podophages, and their genomes are 43,205 and 45,327 bp in length, resembling viruses in the class Caudoviricetes. Their host ranges were very l…
Low-level environmental metal pollution is associated with altered gut microbiota of a wild rodent, the bank vole (Myodes glareolus)
2021
Mining and related industries are a major source of metal pollution. In contrast to the well-studied effects of ex-posure to metals on animal physiology and health, the impacts of environmental metal pollution on the gut mi-crobiota of wild animals are virtually unknown. As the gut microbiota is a key component of host health, it is important to understand whether metal pollution can alter wild animal gut microbiota composition. Using a combination of 16S rRNA amplicon sequencing and quantification of metal levels in kidneys, we assessed whether multi-metal exposure (the sum of normalized levels of fifteen metals) was associated with changes in gut microbiota of wild bank voles (Myodes glar…