Search results for "mitocondri"

showing 10 items of 41 documents

Melatonin Targets Metabolism in Head and Neck Cancer Cells by Regulating Mitochondrial Structure and Function.

2021

This study was funded by grants from the Ministerio de Economia, Industria y Competitividad y por el Fondo de Desarrollo Regional FEDER, Spain nº SAF2013-49019, SAF2017-85903-P, and from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (P07- CTS- 03135, P10- CTS- 5784, and CTS- 101), Spain. J.F. and L.M. have FPU fellowships from the Ministerio de Educación Cultura y Deporte, Spain. C.R.S. was a schorlarship holder from the Plan Propio de Investigación of the University of Granada.

0301 basic medicine:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Phosphorylation::Oxidative Phosphorylation [Medical Subject Headings]PhysiologyClinical BiochemistrymelatoninMitochondrionBiochemistryMelatonina:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]0302 clinical medicine:Anatomy::Cells::Cells Cultured::Cell Line [Medical Subject Headings]head and neck cancer cells:Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance::Drug Resistance Neoplasm [Medical Subject Headings]MitophagyMitocondriasChemistryapoptosisglycolysisOXPHOSmitochondria030220 oncology & carcinogenesishormones hormone substitutes and hormone antagonistsmedicine.drug:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Carbohydrate Metabolism::Glycolysis [Medical Subject Headings]Neoplasias de cabeza y cuello:Diseases::Neoplasms::Neoplasms by Site::Head and Neck Neoplasms [Medical Subject Headings]:Chemicals and Drugs::Inorganic Chemicals::Free Radicals::Reactive Oxygen Species [Medical Subject Headings]Mitofagiafree radicalsOxidative phosphorylationArticleMelatonin03 medical and health sciencesmedicine:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings]Molecular BiologyRadicales libresCell growth:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::DNA-Binding Proteins::Receptors Cytoplasmic and Nuclear::Receptors Melatonin [Medical Subject Headings]:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents [Medical Subject Headings]lcsh:RM1-950:Anatomy::Cells::Cellular Structures::Subcellular Fractions::Mitochondria [Medical Subject Headings]Cell Biologymedicine.diseaseHead and neck squamous-cell carcinoma:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis [Medical Subject Headings]Glucólisis030104 developmental biologylcsh:Therapeutics. PharmacologymitophagyApoptosisCancer cellCancer research:Chemicals and Drugs::Hormones Hormone Substitutes and Hormone Antagonists::Hormones::Melatonin [Medical Subject Headings]
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MYC Induces a Hybrid Energetics Program Early in Cell Reprogramming

2018

Summary Cell reprogramming is thought to be associated with a full metabolic switch from an oxidative- to a glycolytic-based metabolism. However, neither the dynamics nor the factors controlling this metabolic switch are fully understood. By using cellular, biochemical, protein array, metabolomic, and respirometry analyses, we found that c-MYC establishes a robust bivalent energetics program early in cell reprogramming. Cells prone to undergo reprogramming exhibit high mitochondrial membrane potential and display a hybrid metabolism. We conclude that MYC proteins orchestrate a rewiring of somatic cell metabolism early in cell reprogramming, whereby somatic cells acquire the phenotypic plast…

0301 basic medicineCell signalingSomatic cellCèl·lulesCellOxidative phosphorylationcell reprogramming cell signaling metabolism mitochondrial dynamicsBiologyHybrid CellsBiochemistryMitochondrial DynamicsArticleOxidative PhosphorylationMitocondrisProto-Oncogene Proteins c-myc03 medical and health sciencesMetabolomicsCDC2 Protein KinaseGeneticsmedicinecell signalingAnimalsHumansGlycolysisPhosphorylationlcsh:QH301-705.5Membrane potentialMembrane Potential Mitochondriallcsh:R5-920cell reprogrammingCell BiologyCellular ReprogrammingCell biologyMitochondriaMice Inbred C57BL030104 developmental biologymedicine.anatomical_structurelcsh:Biology (General)lcsh:Medicine (General)ReprogrammingmetabolismGlycolysisDevelopmental Biology
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Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming.

2016

During the process of reprogramming to induced pluripotent stem (iPS) cells, somatic cells switch from oxidative to glycolytic metabolism, a transition associated with profound mitochondrial reorganization. Neither the importance of mitochondrial remodelling for cell reprogramming, nor the molecular mechanisms controlling this process are well understood. Here, we show that an early wave of mitochondrial fragmentation occurs upon expression of reprogramming factors. Reprogramming-induced mitochondrial fission is associated with a minor decrease in mitochondrial mass but not with mitophagy. The pro-fission factor Drp1 is phosphorylated early in reprogramming, and its knockdown and inhibition…

0301 basic medicineDynaminsSomatic cellMAP Kinase Signaling SystemScienceCèl·lulesCellInduced Pluripotent Stem CellsKruppel-Like Transcription FactorsGeneral Physics and AstronomyBiologyMitochondrionMitochondrial DynamicsGeneral Biochemistry Genetics and Molecular BiologyMitocondrisArticleCell LineProto-Oncogene Proteins c-myc03 medical and health sciencesKruppel-Like Factor 4MiceMitophagymedicineAnimalsPhosphorylationInduced pluripotent stem cellGeneticsMultidisciplinarySOXB1 Transcription FactorsQGeneral ChemistryCellular ReprogrammingCell biologyMitochondria030104 developmental biologymedicine.anatomical_structurePhosphorylationMitochondrial fissionReprogrammingOctamer Transcription Factor-3Nature communications
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Discovering new proteins in plant mitochondria by RNA editing simulation

2016

In plant mitochondria an essential mechanism for gene expression is RNA editing, often influencing the synthesis of functional proteins. RNA editing alters the linearity of genetic information transfer. Indeed it causes differences between RNAs and their coding DNA sequences that hinder both experimental and computational research of genes. Therefore common software tools for gene search, successfully applied to find canonical genes, often fail in discovering genes encrypted in the genome of plants. Here we propose a novel strategy useful to identify candidate coding sequences resulting from possible editing substitutions. In particular, we consider c!u substitutions leading to the creation…

0301 basic medicineGeneticsMitochondrial DNASequence analysisediting plant mitocondria simulationBiologyGenomeStop codon03 medical and health sciencesOpen reading frame030104 developmental biologyRNA editingGene expressionGene
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Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts

2016

The research leading to these results was funded by the Spanish Ministry of Health (FIS-PI11/00610, FIS-PI041436, FIS-PI081151, FIS-PI042018, and FIS-PI09/02311), the European Union (EU) (FP7-ENV-2011 cod 282957 and HEALTH.2010.2.4.5-1), the Instituto de Salud Carlos III (Red INMA G03/176, CB06/02/0041, FIS-FEDER 03/1615, 04/1509, 04/1112, 04/1931, 05/1079, 05/1052, 06/1213, 07/0314, 09/02647, 11/01007, 11/02591, CP11/00178, FIS-PI06/0867, and FIS-PS09/00090), the Conselleria de Sanitat Generalitat Valenciana, the Generalitat de Catalunya-CIRIT (1999SGR 00241), the Obre Social Cajastur, the Universidad de Oviedo, the Department of Health of the Basque Government (2005111093 and 2009111069),…

0301 basic medicinePediatrics:Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight::Birth Weight [Medical Subject Headings]:Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Morphogenesis::Embryonic and Fetal Development::Fetal Development [Medical Subject Headings]Health Toxicology and MutagenesisPlacentaEspañaPhysiology:Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Growth Disorders::Fetal Growth Retardation [Medical Subject Headings]ADN mitocondrial010501 environmental sciencesMitochondrion01 natural sciencesFetal DevelopmentBélgicaPregnancyBirth Weightskin and connective tissue diseasesPeso al nacerNews | Science SelectionsMitocondrias:Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Reproduction::Pregnancy [Medical Subject Headings]:Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Mitochondria [Medical Subject Headings]2. Zero hunger:Geographicals::Geographic Locations::Europe::Spain [Medical Subject Headings]Air PollutantsAmbient air pollutionAire -- ContaminacióFemenino3. Good healthmedicine.anatomical_structureMaternal ExposureFemaleBirth cohort:Health Care::Environment and Public Health::Public Health::Environmental Pollution::Air Pollution [Medical Subject Headings]Mitochondrial DNAmedicine.medical_specialtyModelos LinealesEmbarazoBirth weightInfants -- DesenvolupamentBiology:Chemicals and Drugs::Inorganic Chemicals::Gases::Nitrogen Oxides::Nitrogen Dioxide [Medical Subject Headings]DNA Mitochondrial:Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Genes Mitochondrial [Medical Subject Headings]03 medical and health sciencesAir pollutantsPlacentaAir PollutionmedicineHumans:Geographicals::Geographic Locations::Europe::Belgium [Medical Subject Headings]:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Models Statistical::Linear Models [Medical Subject Headings]Contaminación del aire0105 earth and related environmental sciencesRetardo del crecimiento fetal:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::DNA::DNA Circular::DNA Mitochondrial [Medical Subject Headings]Genes mitocondrialesPregnancyPublic Health Environmental and Occupational HealthDesarrollo fetalmedicine.disease:Anatomy::Embryonic Structures::Placenta [Medical Subject Headings]030104 developmental biology:Check Tags::Female [Medical Subject Headings]13. Climate actionSpainsense organsDióxido de nitrógeno
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Mitochondrial DNA Replacement Techniques to Prevent Human Mitochondrial Diseases.

2021

Background: Mitochondrial DNA (mtDNA) diseases are a group of maternally inherited genetic disorders caused by a lack of energy production. Currently, mtDNA diseases have a poor prognosis and no known cure. The chance to have unaffected offspring with a genetic link is important for the affected families, and mitochondrial replacement techniques (MRTs) allow them to do so. MRTs consist of transferring the nuclear DNA from an oocyte with pathogenic mtDNA to an enucleated donor oocyte without pathogenic mtDNA. This paper aims to determine the efficacy, associated risks, and main ethical and legal issues related to MRTs. Methods: A bibliographic review was performed on the MEDLINE and Web of S…

0301 basic medicinePoor prognosisLegal positionMitochondrial DNAFarmacologiaWeb of scienceMEDLINEReviewmitochondrial DNABioinformaticsDNA MitochondrialCatalysisMitocondrisInorganic Chemistrylcsh:Chemistry03 medical and health sciencesmitochondrial donation0302 clinical medicineMedicineHumansPhysical and Theoretical ChemistryMolecular Biologylcsh:QH301-705.5Spectroscopymitochondrial diseases030219 obstetrics & reproductive medicinebusiness.industryOrganic ChemistryDonor oocyteGeneral MedicineDNAGenetic TherapyComputer Science ApplicationsNuclear DNAMitochondriaClinical trial030104 developmental biologylcsh:Biology (General)lcsh:QD1-999Oocytesmitochondrial replacementthree-parent babybusinessInternational journal of molecular sciences
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Funciones protectoras de los astrocitos en la inflamación y el estrés oxidativo cerebral

2018

La enfermedad de Alzheimer (EA) es una enfermedad neurodegenerativa que se presenta con mayor prevalencia en la población anciana. Afecta a alrededor de 37 millones de personas en todo el mundo. Desde el punto de vista inmunohistoquímico, la EA se caracteriza por la presencia de placas neuríticas (con el péptido tóxico Aβ1-42, entre otros) y ovillos neurofibrilares en diversas áreas del cerebro, responsables de la pérdida neuronal, el deterioro de las conexiones sinápticas y la gliosis reactiva causando la inflamación característica de esta enfermedad. Resultados previos indican que los astrocitos son células de protección para las neuronas y que podrían proteger de la inflamación y del est…

:CIENCIAS DE LA VIDA::Biología celular::Cultivo celular [UNESCO]:CIENCIAS DE LA VIDA::Bioquímica [UNESCO]estrés oxidativoinflamación:CIENCIAS DE LA VIDA::Neurociencias::Neuroquímica [UNESCO]enfermedad de alzheimerapoptosisbiogénesis mitocondrialUNESCO::CIENCIAS DE LA VIDA::Biología celular::Cultivo celularUNESCO::CIENCIAS DE LA VIDA::BioquímicaUNESCO::CIENCIAS DE LA VIDA::Neurociencias::Neuroquímica
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Overcoming a “Probable” Diagnosis in Antimitochondrial Antibody Negative Primary Biliary Cirrhosis: Study of 100 Sera and Review of the Literature

2010

Serum anti-mitochondrial antibodies (AMA) are the serological hallmark of primary biliary cirrhosis (PBC), yet up to 15% of PBC sera are AMA negative at routine indirect immunofluorescence (IIF) while being referred to as “probable” cases. The diagnostic role of PBC-specific antinuclear antibodies (ANA) remains to be determined. We will report herein data on the accuracy of new laboratory tools for AMA and PBC-specific ANA in a large series of PBC sera that were AMA-negative at IIF. We will also provide a discussion of the history and current status of AMA detection methods. We included IIF AMA-negative PBC sera (n = 100) and sera from patients with other chronic liver diseases (n = 104) th…

AdultMaleAnti-nuclear antibodyNuclear dotsPBCSerologyAnticorpo antimitocondrio cirrosi biliare primitivaPrimary biliary cirrhosisAntigenparasitic diseasesHumansImmunology and AllergyMedicineskin and connective tissue diseasesAgedAutoantibodiesAged 80 and overbiologyLiver Cirrhosis Biliarybusiness.industryAutoantibodyIIfGeneral MedicineMiddle Agedmedicine.diseasedigestive system diseasesMitochondriaImmunologybiology.proteinFemaleAntibodybusinessClinical Reviews in Allergy & Immunology
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Mouse model for Charcot Marie-Tooth as a tool to better understand the disease

2016

Introducción La enfermedad de Charcot-Marie-Tooth (CMT) es uno de los trastornos neurológicos hereditarios más comunes que afecta a 17-40 de cada 100.000 personas según poblaciones; concretamente esta cifra se sitúa en 28 personas de cada 100.000 en España. La enfermedad recibe el nombre de los tres médicos que la identificaron por primera vez en 1886; Jean-Marie Charcot y Pierre Marie en París, Francia y Howard Henry Tooth en Cambridge, Inglaterra. La enfermedad de CMT, también conocida como neuropatía hereditaria motora y sensitiva o atrofia muscular del peroneo, abarca un grupo de trastornos que afectan los nervios periféricos. El fenotipo clásico incluye problemas en la marcha, déficit …

Charcot-Marie-ToothmitocondriaUNESCO::CIENCIAS DE LA VIDAmodelo murino:CIENCIAS DE LA VIDA [UNESCO]
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Estudio del estrés oxidativo en un modelo animal de ejercicio físico: análisis citométrico en mitocondrias aisladas y en leucocitos

2015

Un incremento en el metabolismo aeróbico durante el ejercicio es una fuente potencial de estrés oxidativo. En el músculo, la mitocondria es una fuente importante de intermediarios reactivos. Para su estudio, se han utilizado el hígado, el músculo de diafragma y el corazón de conejos tipo control sin entrenamiento físico específico, conejos entrenados sometidos a un entrenamiento físico específico y conejos sometidos a una operación simulada. Se han obtenido mitocondrias aisladas para su estudio mediante ensayos polarigráficos de consumo de oxígeno o respiración mitocondrial mediante la adición de sustratos oxidativos y ADP y ensayos del potencial de membrana mitocondrial mediante la captaci…

CitometríaUNESCO::CIENCIAS DE LA VIDAEstrés oxidativoEjercicioMitocondrias:CIENCIAS DE LA VIDA [UNESCO]
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