Search results for "mixtures"
showing 10 items of 913 documents
Mutilation of RNA phage Qβ virus-like particles: from icosahedrons to rods
2000
Icosahedral virus-like particles (VLPs) of RNA phage Qbeta are stabilized by four disulfide bonds of cysteine residues 74 and 80 within the loop between beta-strands F and G (FG loop) of the monomeric subunits, which determine the five-fold and quasi-six-fold symmetry contacts of the VLPs. In order to reduce the stability of Qbeta VLPs, we mutationally converted the amino acid stretch 76-ANGSCD-81 within the FG loop into the 76-VGGVEL-81 sequence. It led to production in Escherichia coli cells of aberrant rod-like Qbeta VLPs, along with normal icosahedral capsids. The length of the rod-like particles exceeded 4-30 times the diameter of icosahedral Qbeta VLPs.
Approaches to find complementary separation conditions for resolving complex mixtures by high-performance liquid chromatography
2012
Chromatographic problems are usually addressed trying to find out a single experimental condition aimed to resolve all compounds in the sample. However, very often, the chromatographic system is not able to provide full resolution. When a separation fails, the usual choice is introducing a drastic change in the chromatographic system (e.g. column, solvent, pH). There are, however, other possibilities that take advantage of the gathered information in the failed separation, without the need of new experiments, based on the concept of complementary separations (e.g. isocratic mobile phases, gradients, columns, chromatographic modes). One separation condition will focus on the resolution of so…
Keyhole limpet hemocyanin (KLH): a biomedical review
1999
In this review we present a broad survey of fundamental scientific and medically applied studies on keyhole limpet hemocyanin (KLH). Commencing with the biochemistry of KLH, information on the biosynthesis and biological role of this copper-containing respiratory protein in the marine gastropod Megathura crenulata is provided. The established methods for the purification of the two isoforms of KLH (KLH1 and KLH2) are then covered, followed by detailed accounts of the molecular mass determination, functional unit (FU) structure, carbohydrate content, immunological analysis and recent aspects of the molecular genetics of KLH. The transmission electron microscope (TEM) has contributed signific…
Review of 8 years of experience with Infanrix hexa (DTPa-HBV-IPV/Hib hexavalent vaccine).
2009
Combination vaccines that include multiple antigens within one formulation are now widely accepted as an effective means of eliciting protection against several diseases at the same time. Owing to improvements in quality and convenient modes of administration, they have become part of routine pediatric practice. Hexavalent vaccines, including diphtheria, tetanus, pertussis, hepatitis B, polio and Haemophilus influenzae type b antigens represent the latest advance in the development of combination vaccines. Over 8 years since its first licensure, this review looks at the immunogenicity, efficacy and safety profile of the only hexavalent pediatric vaccine currently in use--Infanrix hexa (diph…
"Table 51" of "Measurement of inclusive jet charged-particle fragmentation functions in Pb+Pb collisions at sqrt(s_NN) = 2.76 TeV with the ATLAS dete…
2015
Ratio of D(z) distributions for R=0.3 jets for central to peripheral events.
"Table 56" of "Measurement of inclusive jet charged-particle fragmentation functions in Pb+Pb collisions at sqrt(s_NN) = 2.76 TeV with the ATLAS dete…
2015
Ratio of D(z) distributions for R=0.3 jets for central to peripheral events.
"Table 60" of "Measurement of inclusive jet charged-particle fragmentation functions in Pb+Pb collisions at sqrt(s_NN) = 2.76 TeV with the ATLAS dete…
2015
Ratio of D(z) distributions for R=0.2 jets for central to peripheral events.
"Table 62" of "Measurement of inclusive jet charged-particle fragmentation functions in Pb+Pb collisions at sqrt(s_NN) = 2.76 TeV with the ATLAS dete…
2015
Ratio of D(z) distributions for R=0.2 jets for central to peripheral events.
"Table 47" of "Measurement of inclusive jet charged-particle fragmentation functions in Pb+Pb collisions at sqrt(s_NN) = 2.76 TeV with the ATLAS dete…
2015
Ratio of D(z) distributions for R=0.4 jets for central to peripheral events.
"Table 45" of "Measurement of inclusive jet charged-particle fragmentation functions in Pb+Pb collisions at sqrt(s_NN) = 2.76 TeV with the ATLAS dete…
2015
Ratio of D(z) distributions for R=0.4 jets for central to peripheral events.