Search results for "molecular markers"

Molecular characterization of the Sicilian goat populations

2014

The Sicilian goats are characterized by a strong population admixture structure, caused by geographical location of the farms, influences of natural mating and traditional breeding systems where flock represents an important breeding unit. Nowadays, several local populations are reared in Sicily, some of which do not have a recognized defined genetic structure, having taken origin from several crosses between animals of different breeds/populations sharing the same environment. The aim of this work was to characterize the Sicilian autochthonous goat populations using microsatellite markers and genetic polymorphisms of casein genes

“Identification of molecular and genetic markers common to multifactorial diseases (cardiovascular diseases, metabolic disorders, tumors) associated …

Somatic embryogenesis through in vitro anther culture ofCitrus sinensisL. Osbeck ‘Moro’

2019

In many crops, anther culture is the most used method to induce gametic embryogenesis, aimed to regenerate homozygous plants. However, also somatic embryogenesis can be obtained by this method, when somatic tissue is involved in regeneration process. Many factors can affect this procedure, such as genotype, pre-treatments applied to floral buds, pollen developmental stage, donor plant states, culture media composition and growth culture conditions. Anthers of Citrus sinensis L. Osbeck, cv. Moro, were collected at the vacuolate stage, and after a chilling (4°C) pre-treatment of 7 days, were placed on the same medium, evaluating different temperature stresses applied after the placing in cult…

Application of microsatellite markers as potential tools for traceability of Girgentana goat breed dairy products

2015

In livestock, breed assignment may play a key role in the certification of products linked to specific breeds. Traceability of farm animals and authentication of their products can contribute to improve breed profitability and sustainability of animal productions with significant impact on the rural economy of particular geographic areas and on breed and biodiversity conservation. With the goal of developing a breed genetic traceability system for Girgentana dairy products, the aim of this study was to identify specific microsatellite markers able to discriminate among the most important Sicilian dairy goat breeds, in order to detect possible adulteration in Girgentana dairy products. A tot…

New polymorphic markers for genetic diversity studies in an invasive plant: the common ragweed (Ambrosia artemisiifolia L.)

2014

Common ragweed is an annual herb that belongs to the Asteraceae family. It is known as an invasive plant originating from the USA. In France the common ragweed is especially abundant in the Rhône-Alpes basin and currently spreads northwards in Burgundy. Ragweed colonizes different types of environments, such as railways, river sides, wastelands, farmlands and cultivated crops (especially sunflowers crops). Herbicide resistance to linuron, glyphosate and/or acetolactate synthase inhibitors has been reported in the USA, which complicates the chemical control of ragweed. In addition, the highly allergenic pollen of ragweed causes severe allergies. These elements make this plant a major threat …

Fusariose du Cyclamen (FoCy): Détection préventive du risque et contrôle biologique

2013

Fusarium oxysporum f. sp. cyclaminis: Identification of specific molecular markers for improved disease prevention

2014

Fusarium oxysporum is a well-known soil-borne and ubiquitous fungus. The species includes a high diversity of saprophytic and pathogenic populations. The phytopathogenic ones are subdivided into formae speciales according to their host specificity. The forma specialis cyclaminis (FoCy) is causing Fusarium wilt only on cyclamen. It causes damages during the entire production line and can raise more than 50 % losses. FoCy is considered as the more destructive pathogen on cyclamen. To achieve a better understanding of the disease and a more efficient and eco-friendly way of control, we aim at finding a specific molecular marker allowing for early detection and quantification of the pathogen an…

In vitro rescue of zygotic embryos of sour orange, Citrus aurantium L., and their detection based on RFLP analysis

1998

Embryo development in vivo has been studied in four Citrus aurantium L. polyembryonic genotypes. Seeds were collected 65, 85, 105, 125 and 220 days after pollination (DAP). None of the immature seeds harvested 65 and 85 DAP contained visible embryos. A single embryo at a more advanced developmental stage was observed in the central position at the micropylar apex of the embryo sac in about 74% of seeds harvested at 105 DAP, while at 125 and 220 DAP the majority of seeds had two or more embryos at the same developmental stage crowded together. Restriction fragment length polymorphism (RFLP) analysis of low- and high-copy-number nuclear DNA was used to distinguish zygotic from nucellar seedli…

On the origin and diversification of Podolian cattle breeds: testing scenarios of European colonization using genome-wide SNP data

2021

AbstractBackgroundDuring the Neolithic expansion, cattle accompanied humans and spread from their domestication centres to colonize the ancient world. In addition, European cattle occasionally intermingled with both indicine cattle and local aurochs resulting in an exclusive pattern of genetic diversity. Among the most ancient European cattle are breeds that belong to the so-called Podolian trunk, the history of which is still not well established. Here, we used genome-wide single nucleotide polymorphism (SNP) data on 806 individuals belonging to 36 breeds to reconstruct the origin and diversification of Podolian cattle and to provide a reliable scenario of the European colonization, throug…

Molecular markers and endometrial cancer

2022

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