Search results for "motifs"
showing 10 items of 180 documents
Motivic Pattern Extraction in Music, and Application to the Study of Tunisian Modal Music
2007
A new methodology for automated extraction of repeated patterns in time-series data is presented, aimed in particular at the analysis of musical sequences. The basic principles consists in a search for closed patterns in a multi-dimensional parametric space. It is shown that this basic mechanism needs to be articulated with a periodic pattern discovery system, implying therefore a strict chronological scanning of the time-series data. Thanks to this modelling global pattern filtering may be avoided and rich and highly pertinent results can be obtained. The modelling has been integrated in a collaborative pro ject between ethnomusicology, cognitive sciences and computer science, aimed at the…
New Melanocortin 1 Receptor Binding Motif Based on the C-Terminal Sequence of ?-Melanocyte-Stimulating Hormone
2006
The C-terminal tripeptide of the alpha-melanocyte stimulating hormone (alpha-MSH11-13) possesses strong antiinflammatory activity without known cellular target. In order to better understand the structural requirements for function of such motif, we designed, synthesized and tested out Trp- and Tyr-containing analogues of the alpha-MSH11-13. Seven alpha-MSH11-13 analogues were synthesized and characterized for their binding to the melanocortin receptors recombinantly expressed in insect (Sf9) cells, infected with baculovirus carrying corresponding MC receptor DNA. We also tested these analogues on B16-F1 mouse melanoma cells endogenously expressing the MC1 receptor for binding and for abili…
α-secretase mediated conversion of the amyloid precursor protein derived membrane stub C99 to C83 limits Aβ generation
2009
The Swedish mutation within the amyloid precursor protein (APP) causes early-onset Alzheimer's disease due to increased cleavage of APP by BACE1. While beta-secretase shedding of Swedish APP (APPswe) largely results from an activity localized in the late secretory pathway, cleavage of wild-type APP occurs mainly in endocytic compartments. However, we show that liberation of Abeta from APPswe is still dependent on functional internalization from the cell surface. Inspite the unchanged overall beta-secretase cleaved soluble APP released from APP(swe) secretion, mutations of the APPswe internalization motif strongly reduced C99 levels and substantially decreased Abeta secretion. We point out t…
Translation of HTT mRNA with expanded CAG repeats is regulated by the MID1-PP2A protein complex.
2012
Expansion of CAG repeats is a common feature of various neurodegenerative disorders, including Huntington's disease. Here we show that expanded CAG repeats bind to a translation regulatory protein complex containing MID1, protein phosphatase 2A and 40S ribosomal S6 kinase. Binding of the MID1-protein phosphatase 2A protein complex increases with CAG repeat size and stimulates translation of the CAG repeat expansion containing messenger RNA in a MID1-, protein phosphatase 2A- and mammalian target of rapamycin-dependent manner. Our data indicate that pathological CAG repeat expansions upregulate protein translation leading to an overproduction of aberrant protein and suggest that the MID1-com…
Metalotioneiny i motywy policysteinylowe : oddziaływanie z jonami metali
2018
Many of biochemical paths and processes require some metal ions to occur. There are also known the negative effects of the presence of metal ions in the organism. The both sides of metal ions interactions on the living organism require specific regulations and cannot be left without supervision and control of the organism itself. One of the strategy to keep the control on metal ions are cystein-rich proteins that play crucial role in detoxication of metal ions that are dangerous for human organism as well as they help to maintain homeostasis of essential metal ions. Matallothioneins are one of the well known, but still not fully understood, cysteine- rich proteins. They are small proteins b…
Recursion at the crossroads of sequence modeling, random trees, stochastic algorithms and martingales
2013
This monograph synthesizes several studies spanning from dynamical systems in the statistical analysis of sequences, to analysis of algorithms in random trees and discrete stochastic processes. These works find applications in various fields ranging from biological sequences to linear regression models, branching processes, through functional statistics and estimates of risk indicators for insurances. All the established results use, in one way or another, the recursive property of the structure under study, by highlighting invariants such as martingales, which are at the heart of this monograph, as tools as well as objects of study.
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…
Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry
2012
ABSTRACT Adeno-associated virus type 2 (AAV2) has gained much interest as a gene delivery vector. A hallmark of AAV2-mediated gene transfer is an intracellular conformational change of the virus capsid, leading to the exposure of infection-relevant protein domains. These protein domains, which are located on the N-terminal portion of the structural proteins VP1 and VP2, include a catalytic phospholipase A 2 domain and three clusters of basic amino acids. We have identified additional protein sequence motifs located on the VP1/2 N terminus that also proved to be obligatory for virus infectivity. These motifs include signals that are known to be involved in protein interaction, endosomal sort…
Polonica we wspomnieniach Zofii Kowalewskiej
2016
The present article focuses on Polish motifs in Sofya Kovalevskaya’s recollections. Kovalevskaya was a great 19th century Russian mathematician whose contributions were acknowledged throughout Europę during her lifetime. She was the first European woman to receive a doctorate in mathematics from the University of Gottingen, a professor at Stockholm University in Sweden, and the first female corresponding member of the Russian Academy of Sciences. Besides her scientific activities, Kovalevskaya was also a writer and publicist. She is the author of a number of novels, poems, a play and autobiographical Recollections of Childhood. In one of the chapters, entitled “Polish Uprising Recollections…
O wierszach (nie tylko) dla dzieci Juliana Kornhausera
2018
The article is based on a synthetic description of this part of Julian Komhauser’s oeuvre, in which the figure of a child appears. Thus, it focuses on the interpretation of a selection of the poet’s texts that are addressed to children exclusively. A significant part is therefore devoted to considerations on the poems that are situated in the borderland between literature for children and texts for adults. The major synthetic aim of the article is an attempt to divide Kornhauser’s “children’s poems” into two main categories: carnivalesque, as well as reflexive and symbolic ones.