Search results for "mutagen"

showing 10 items of 2607 documents

Mycotoxin Analysis of Human Urine by LC-MS/MS: A Comparative Extraction Study

2017

The lower mycotoxin levels detected in urine make the development of sensitive and accurate analytical methods essential. Three extraction methods, namely salting-out liquid–liquid extraction (SALLE), miniQuEChERS (quick, easy, cheap, effective, rugged, and safe), and dispersive liquid–liquid microextraction (DLLME), were evaluated and compared based on analytical parameters for the quantitative LC-MS/MS measurement of 11 mycotoxins (AFB1, AFB2, AFG1, AFG2, OTA, ZEA, BEA, EN A, EN B, EN A1 and EN B1) in human urine. DLLME was selected as the most appropriate methodology, as it produced better validation results for recovery (79–113%), reproducibility (RSDs < 12%), and repeatability (RSDs…

AdultHealth Toxicology and MutagenesisLiquid-Liquid Extractionlcsh:MedicineUrineToxicology01 natural sciencesArticlechemistry.chemical_compound0404 agricultural biotechnologyTandem Mass SpectrometrymycotoxinsLc ms msHealthy volunteersHumansLC-MS/MSMycotoxinReproducibilityChromatographymycotoxins; urine; optimization; method validation; LC-MS/MSlcsh:R010401 analytical chemistryExtraction (chemistry)method validation04 agricultural and veterinary sciencesRepeatability040401 food scienceurine0104 chemical scienceschemistryExtraction methodsoptimizationChromatography LiquidToxins
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Nucleoplasmic bridges and acrocentric chromosome associations as early markers of exposure to low levels of ionising radiation in occupationally expo…

2014

Ionising radiation, with the contribution of telomere shortening, induces DNA double-strand breaks that result in chromosome end fusion, nucleoplasmic bridges (NPBs) and chromosome aberrations (ChAbs) as well as dicentric chromosomes. In order to investigate the chromosomal damage induced by occupational ionising radiation at low exposure levels, and to find early markers of health hazard, peripheral lymphocytes of occupationally exposed hospital workers were cytogenetically analysed. Results showed a significant difference in the frequency of ChAbs in exposed subjects relative to controls. A significant number of NPBs between nuclei of binucleated cultured lymphocytes from exposed subjects…

AdultHealth Toxicology and MutagenesisLymphocyteBiologyToxicologyIonizing radiationAndrologyDicentric chromosomeOccupational ExposureRadiation IonizingCentromereGeneticsmedicineHumansLymphocytesIn Situ Hybridization FluorescenceGenetics (clinical)Cell NucleusChromosome AberrationsChromosomeEarly cytogenetics markers hospital workersMolecular biologyTelomereChromatinPersonnel HospitalSettore BIO/18 - GeneticaCell nucleusmedicine.anatomical_structureBiomarkersMutagenesis
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Validation of the Brief Perceived Positive Lockdown Impact Scale PPLIS-4

2022

Background: Although research showed that positive aspects of the lockdown were perceived during the pandemic, there are no tools to test the positive impact of mandatory social isolation on life. The present study aims to validate a newly developed, brief, four-item perceived positive lockdown impact scale (PPLIS-4). Methods: A cross-sectional online survey study was formed among 4370 adults in three samples: Sample 1 consisted of university students from Poland and Ukraine, Sample 2 consisted of Polish university students under 26 (emerging adults), and Sample 3 consisted of Polish and Ukrainian adults above 25 (non-emerging adults). The standardized questionnaire was used for criterion v…

AdultHealth Toxicology and Mutagenesisemerging adultsPublic Health Environmental and Occupational HealthCOVID-19factor analysiscross-cultural validation studycross-cultural validation study; emerging adults; factor analysis; the positive impact of the COVID-19 pandemic; university students; well-beingCross-Sectional Studieswell-beingSurveys and QuestionnairesCommunicable Disease Controlthe positive impact of the COVID-19 pandemicHumansuniversity studentsPandemicsInternational Journal of Environmental Research and Public Health
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Distributions and determinants of urinary biomarkers of organophosphate pesticide exposure in a prospective Spanish birth cohort study

2017

Background: Prenatal exposure to organophosphate pesticides (OPs) has been associated with impaired child development. Pesticide exposure determinants need to be studied in order to identify sources and pathways of pesticide exposure. The aim of this paper is to describe prenatal exposure to OPs and evaluate the associated factors in pregnant women. Methods: The study population consisted of pregnant women ( n = 573) who participated in the INMA birth cohort study in Valencia (Spain, 2003 – 2006). OP metabolites were analyzed in maternal urine at the 32nd week of gestation using a liquid chromatography-high resolution mass spectrometry method. The analysis included non-specific (diethyl pho…

AdultInsecticidesmedicine.medical_specialtyHealth Toxicology and Mutagenesis010501 environmental sciencesDevelopment01 natural sciencesCohort StudiesToxicologylcsh:RC963-96903 medical and health scienceschemistry.chemical_compoundOrganophosphorus Compounds0302 clinical medicinePregnancyEnvironmental healthVegetablesmedicineHumans030212 general & internal medicinePesticides0105 earth and related environmental sciences2. Zero hungerPregnancyFetusbusiness.industrylcsh:Public aspects of medicineResearchPublic healthOrganophosphatePublic Health Environmental and Occupational Healthlcsh:RA1-1270Agriculturemedicine.disease3. Good healthDietTCPychemistryMaternal ExposureSpainFruitlcsh:Industrial medicine. Industrial hygieneGestationPopulation studyEnvironmental PollutantsFemalebusinessBody mass index
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Working Alliance Inventory for Online Interventions-Short Form (WAI-TECH-SF): The Role of the Therapeutic Alliance between Patient and Online Program…

2020

Background: Therapeutic alliance (TA) between the patient and therapist has been related to positive therapeutic outcomes. Because Internet-based interventions are increasingly being implemented, a tool is needed to measure the TA with Internet-based self-guided programs. The Working Alliance Inventory for online interventions (WAI-TECH-SF) was adapted based on the WAI Short Form (Hatcher &amp

AdultMale020205 medical informaticsAdolescentPsychometricsonline interventionsTherapeutic AllianceHealth Toxicology and MutagenesisPsychological interventionlcsh:MedicineTherapeutic alliance02 engineering and technologytherapeutic outcomesArticle03 medical and health sciencesYoung Adult0302 clinical medicineBehavior TherapyInternal consistencySurveys and QuestionnairesCredibility0202 electrical engineering electronic engineering information engineeringHumans030212 general & internal medicineOnline interventionsDepressive symptomsAgedTherapeutic outcomesDepressionlcsh:RPublic Health Environmental and Occupational HealthReproducibility of ResultsProfessional-Patient RelationsMiddle AgedExploratory factor analysis3. Good healthAllianceTreatment OutcomeSatisfaction with the treatmentPatient Satisfactionsatisfaction with the treatmentFemalePsychologyInternet-Based InterventionClinical psychologyInternational journal of environmental research and public health
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Blood levels of nitric oxide and DNA breaks assayed in whole blood and isolated peripheral blood mononucleated cells in patients with multiple sclero…

2019

Abstract Oxidative stress, especially overproduction of nitric oxide (NO), is considered to be one of the crucial factors in the pathogenesis of multifactorial multiple sclerosis (MS). DNA breaks could be one of the consequences of oxidative stress; however, data on DNA breakage in MS are very few and contradictory. There are no data on direct measurements of NO production in the blood of MS patients. The goal of this study was to determine the level of single-stranded DNA breaks in whole blood or isolated peripheral blood mononuclear cells (PBMNCs) by means of alkaline single cell gel electrophoresis (comet assay) and to evaluate production of NO in the human blood by applying electron par…

AdultMale0301 basic medicineMultiple SclerosisDNA damageHealth Toxicology and Mutagenesis010501 environmental sciencesNitric Oxidemedicine.disease_cause01 natural sciencesPeripheral blood mononuclear cellNitric oxide03 medical and health scienceschemistry.chemical_compoundGeneticsmedicineHumansDNA Breaks Single-StrandedAged0105 earth and related environmental sciencesWhole bloodGel electrophoresisChemistryMultiple sclerosisElectron Spin Resonance SpectroscopyMiddle Agedmedicine.diseaseMolecular biologyComet assay030104 developmental biologyLeukocytes MononuclearFemaleComet AssaySingle-Cell AnalysisOxidative stressDNA DamageMutation Research/Genetic Toxicology and Environmental Mutagenesis
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The Importance of Considering Alexithymia during Initial Stages of Intimate Partner Violence Interventions to Design Adjuvant Treatments

2019

Empirical evidence supports that individuals with alexithymia might be prone to certain types of violence, such as intimate partner violence against women (IPVAW). Moreover, considering that alexithymia is directly involved in behavior regulation, problems due to identifying and regulating emotional states might be postulated as responsible, at least in part, for the success of psychotherapeutic treatments designed for decreasing the future risk of reoffending. Therefore, we assessed whether alexithymia was a good predictor of the discontinuation of treatment (dropout) and the risk of recidivism during the initial stages of intervention in a sample of men convicted of IPVAW perpetration (n …

AdultMale050103 clinical psychologydrug misuseHealth Toxicology and MutagenesisEmotionsPsychological interventionPoison controlIntimate Partner Violencelcsh:MedicineImpulsivitydropoutArticle03 medical and health sciences0302 clinical medicineAlexithymiaInjury preventionmedicineHumans0501 psychology and cognitive sciencesAffective Symptomsintimate partner violence against womenRecidivism05 social scienceslcsh:RPublic Health Environmental and Occupational HealthreoffendingHuman factors and ergonomicsMiddle AgedDones maltractadesmedicine.diseaseSocioeconomic FactorsDomestic violencemedicine.symptomDroguesalexithymiaPsychology030217 neurology & neurosurgeryClinical psychologyInternational Journal of Environmental Research and Public Health
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Dropout from Court-Mandated Intervention Programs for Intimate Partner Violence Offenders: The Relevance of Alcohol Misuse and Cognitive Impairments

2019

There is considerable interest in offering insight into the mechanisms that might explain why certain perpetrators of intimate partner violence against women (IPVAW) drop out of interventions. Although several socio-demographic variables and attitudes towards IPVAW have been proposed as risk factors for IPVAW perpetrators&rsquo

AdultMale050103 clinical psychologymedicine.medical_specialtyHealth Toxicology and Mutagenesisintimate partner violencePsychological interventionlcsh:MedicinedropoutArticle03 medical and health sciences0302 clinical medicineBehavior TherapyRisk FactorsIntervention (counseling)medicineHumansCognitive Dysfunction0501 psychology and cognitive sciencesPsychiatryempathyDropout (neural networks)Consumption (economics)recidivismRecidivismalcohollcsh:R05 social sciencesPublic Health Environmental and Occupational HealthCognitionDiscontinuationAttitudeParellaDomestic violenceFemaleAlcoholPsychologyAlcohol-Related Disorders030217 neurology & neurosurgeryInternational Journal of Environmental Research and Public Health
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Emotional and Behavioral Consequences of the COVID-19 Pandemic: The Role of Health Anxiety, Intolerance of Uncertainty, and Distress (In)Tolerance.

2020

The COVID-19 pandemic represents a worldwide threat to mental health. To optimize the allocation of health care resources, research on specific vulnerability factors, such as health anxiety, intolerance of uncertainty, and distress (in)tolerance, and particularly their effect on the time course of SARS-CoV-2 related anxiety appears crucial for supporting high risk groups suffering from elevated mental distress during the pandemic. N = 887 participants (78.4% female

AdultMale050103 clinical psychologysafety behaviorsCoronavirus disease 2019 (COVID-19)Health Toxicology and MutagenesisPneumonia Virallcsh:Medicinevirus anxietyAnxietyPsychological DistressArticle03 medical and health sciencesMental distress0302 clinical medicineGermanyPandemicHealth careSafety behaviorsintolerance of uncertaintyMedicineHumans0501 psychology and cognitive sciencesPandemicsbusiness.industry05 social scienceslcsh:RPublic Health Environmental and Occupational HealthUncertaintyCOVID-19Middle AgedMental healthDistresshealth anxietyAnxietyFemaledistress tolerancemedicine.symptombusinessCoronavirus InfectionsAttitude to Health030217 neurology & neurosurgeryClinical psychologyInternational journal of environmental research and public health
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Changes in Alcohol Consumption Pattern Based on Gender during COVID-19 Confinement in Spain

2021

(1) The goal of this study was to analyze the prevalence and pattern of alcohol consumption (frequency of consumption, average daily consumption, and risky consumption) before and during confinement due to the coronavirus disease (COVID-19) in the adult population and based on gender. (2) Methods: Data from 3779 individuals were collected via a set of online surveys. The AUDIT alcohol consumption questions (AUDIT-C) were used to measure the frequency of consumption, the average daily consumption, intensive consumption, risky consumption, and standard drink units. (3) Results: During confinement, the prevalence of alcohol consumption declined in both males and females, but only intensive con…

AdultMale2019-20 coronavirus outbreakmedicine.medical_specialty6114.06 Comportamiento del ConsumidorCoronavirus disease 2019 (COVID-19)Alcohol DrinkingHealth Toxicology and Mutagenesis3309.01 Bebidas Alcohólicasrisk consumptionHealth BehaviorAdult populationFrequency of use030508 substance abusestandard drink unit (SDU)Article03 medical and health sciences0302 clinical medicine6302.02 Psicología SocialSurveys and QuestionnairesgenderMedicineHumans030212 general & internal medicineConsumption (economics)business.industryalcoholSARS-CoV-2Public healthPublic Health Environmental and Occupational HealthRCOVID-19confinement measures63 SociologíaStandard drinkSpainMedicineFemale0305 other medical sciencebusinessAlcohol consumptionDemographyInternational Journal of Environmental Research and Public Health
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