Search results for "mutation."
showing 10 items of 2808 documents
Analytical strategy photodegradation/chemiluminescence/continuous-flow multicommutation methodology for the determination of the herbicide Propanil
2006
The present paper is dealing with an analytical strategy based on coupling photodegradation, chemiluminescence and multicommutation continuous-flow methodology for the determination of the pesticide Propanil, a common herbicide. The pesticide solution is inserted as small segments sequentially alternated with segments of the solution for adjusting the suitable medium for the photodegradation. Both flow-rates (sample and medium) are adjusted to required time for photodegradation, 2.0 min; and then, the resulting solution is also sequentially inserted as segments alternated with segments of the oxidizing solutions system, 1.00 x 10(-4) mol 1(-1) potassium permanganate in 2.00 mol 1(-1) sulphu…
A chemiluminescence flow-based procedure for determination of carbaryl in natural waters exploiting multicommutation and enzymatic reaction
2007
A chemiluminescence procedure for the determination of carbaryl in natural waters using acetylcholinesterase and choline oxidase is described. The flow system designed to implement multicommutation approach controlled by microcomputer comprised five solenoid valves, two columns with immobilized enzymes on controlled pore glass beads and chemiluminometric flow cell. In the best experimental conditions a linear response ranging from 25 to 700 µg L-1 carbaryl was obtained. Water samples were spiked with carbaryl in order to access the accuracy and recoveries between 95 and 101% were obtained for a concentration level ranging from 25 to 100 µg L-1 carbaryl. Detection limit and variation coeffic…
Pulsed EPR determination of water accessibility to spin-labeled amino acid residues in LHCIIb.
2009
Membrane proteins reside in a structured environment in which some of their residues are accessible to water, some are in contact with alkyl chains of lipid molecules, and some are buried in the protein. Water accessibility of residues may change during folding or function-related structural dynamics. Several techniques based on the combination of pulsed electron paramagnetic resonance (EPR) with site-directed spin labeling can be used to quantify such water accessibility. Accessibility parameters for different residues in major plant light-harvesting complex IIb are determined by electron spin echo envelope modulation spectroscopy in the presence of deuterated water, deuterium contrast in …
Congenital secretory diarrhoea caused by activating germline mutations in GUCY2C
2016
Objective Congenital sodium diarrhoea (CSD) refers to a form of secretory diarrhoea with intrauterine onset and high faecal losses of sodium without congenital malformations. The molecular basis for CSD remains unknown. We clinically characterised a cohort of infants with CSD and set out to identify disease-causing mutations by genome-wide genetic testing. Design We performed whole-exome sequencing and chromosomal microarray analyses in 4 unrelated patients, followed by confirmatory Sanger sequencing of the likely disease-causing mutations in patients and in their family members, followed by functional studies. Results We identified novel de novo missense mutations in GUCY2C, the gene encod…
Mast cells promote homeostasis by limiting endothelin-1-induced toxicity
2004
Endothelin-1 (ET-1) is a 21-amino-acid peptide, derived from vascular endothelial cells, with potent vasoconstrictor activity. ET-1 has been implicated in diverse physiological or pathological processes, including the vascular changes associated with sepsis. However, the factors that regulate ET-1-associated toxicity during bacterial infections, or in other settings, are not fully understood. Both the pathology associated with certain allergic and autoimmune disorders, and optimal host defence against bacterial and parasitic infections are mediated by mast cells. In vitro, mast cells can produce ET-1 (ref. 11), undergo ET-1-dependent and endothelin-A receptor (ET(A))-dependent activation, a…
Innovative and Applied Research in Biology: Proceedings
2022
The collection contains SCIENTIFIC articles on the topics of the LU 80 conference reports. The main focus is on innovative and applied research in biology and interdisciplinary fields.
Longest Motifs with a Functionally Equivalent Central Block
2004
International audience; This paper presents a generalization of the notion of longest repeats with a block of k don't care symbols introduced by [Crochemore et al., LATIN 2004] (for k fixed) to longest motifs composed of three parts: a first and last that parameterize match (that is, match via some symbol renaming, initially unknown), and a functionally equivalent central block. Such three-part motifs are called longest block motifs. Different types of functional equivalence, and thus of matching criteria for the central block are considered, which include as a subcase the one treated in [Crochemore et al., LATIN 2004] and extend to the case of regular expressions with no Kleene closure or …
On fixed points of the Burrows-Wheeler transform
2017
The Burrows-Wheeler Transform is a well known transformation widely used in Data Compression: important competitive compression software, such as Bzip (cf. [1]) and Szip (cf. [2]) and some indexing software, like the FM-index (cf. [3]), are deeply based on the Burrows Wheeler Transform. The main advantage of using BWT for data compression consists in its feature of "clustering" together equal characters. In this paper we show the existence of fixed points of BWT, i.e., words on which BWT has no effect. We show a characterization of the permutations associated to BWT of fixed points and we give the explicit form of fixed points on a binary ordered alphabet a, b having at most four b's and th…
Divisible designs from semifield planes
2002
AbstractWe give a general method to construct divisible designs from semifield planes and we use this technique to construct some divisible designs. In particular, we give the case of twisted field plane as an example.
A bijection between words and multisets of necklaces
2012
Two of the present authors have given in 1993 a bijection Phi between words on a totally ordered alphabet and multisets of primitive necklaces. At the same time and independently, Burrows and Wheeler gave a data compression algorithm which turns out to be a particular case of the inverse of Phi. In the present article, we show that if one replaces in Phi the standard permutation of a word by the co-standard one (reading the word from right to left), then the inverse bijection is computed using the alternate lexicographic order (which is the order of real numbers given by continued fractions) on necklaces, instead of the lexicographic order as for Phi(-1). The image of the new bijection, ins…