Search results for "nap"
showing 10 items of 2226 documents
The histone acetyltransferase MOF activates hypothalamic polysialylation to prevent diet-induced obesity in mice
2014
Overfeeding causes rapid synaptic remodeling in hypothalamus feeding circuits. Polysialylation of cell surface molecules is a key step in this neuronal rewiring and allows normalization of food intake. Here we examined the role of hypothalamic polysialylation in the long-term maintenance of body weight, and deciphered the molecular sequence underlying its nutritional regulation. We found that upon high fat diet (HFD), reduced hypothalamic polysialylation exacerbated the diet-induced obese phenotype in mice. Upon HFD, the histone acetyltransferase MOF was rapidly recruited on the St8sia4 polysialyltransferase-encoding gene. Mof silencing in the mediobasal hypothalamus of adult mice prevented…
Magnetic Resonance Imaging Derived Biomarkers of IDH Mutation Status and Overall Survival in Grade III Astrocytomas
2020
The evaluation of the isocitrate dehydrogenase (IDH) mutation status in the glioma decision-making process has diagnostic, prognostic and therapeutic implications. The aim of this study was to evaluate whether conventional magnetic resonance imaging (MRI) and apparent diffusion coefficient (ADC) can noninvasively predict the most common IDH mutational status (R132H) in GIII-astrocytomas and the overall survival (OS). Hence, twenty-two patients (9-F, 13-M) with a histological diagnosis of GIII-astrocytoma and evaluation of IDH-mutation status (12-wild type, 10-mutant) were retrospectively evaluated. Imaging studies were reviewed for the morphological feature and mean ADC values (ADCm). Stati…
Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres
2015
Abstract Although cancer stem-like cells (CSCs) are rare, they can enter a non-proliferative or dormant state and resist therapy. Furthermore, quiescent CSCs are responsible for metastases that can appear after curative surgical treatment of a primary tumor. Because of drug resistance of CSCs, the development of novel therapies is urgently required that specifically target CSCs. Purpose The aim of the present study was to investigate the potential of a panel of natural products and derivatives to inhibit CSC-enriched mammospheres of MCF-7 breast cancer cells. Methods CD44high/CD24low cells were identified by flow cytometry and maintained as mammospheres. As a control, we used two clinically…
A single mutation in the recombinant light chain of tetanus toxin abolishes its proteolytic activity and removes the toxicity seen after reconstituti…
1994
Specific proteolysis by the tetanus toxin light chain of a vesicle-associated membrane protein (VAMP) involved in exocytosis is thought to underlie its intracellular blockade of neurotransmitter release. To substantiate this mechanism, recombinant light chain was expressed as a maltose binding protein-light chain fusion product in Escherichia coli. After purification of affinity chromatography and cleavage with factor Xa, the resultant light chain was isolated and its identity confirmed by Western blotting and N-terminal sequencing. It exhibited activity similar to that of the native light chain in proteolyzing its target in isolated bovine small synaptic vesicles and in hydrolyzing a 62-re…
Metformīna efekti uz mikrogliozi, holīnerģisko neirotransmisiju un sinapšu blīvumu pie sporādiskas Alcheimera slimības tipa patoloģijas
2020
Šajā pētījumā noteica perorāli ievadīta metformīna (75 mg/kg un 100 mg/kg devās) efektus uz vairākiem sporādiskās Alcheimera slimības tipa modeļžurku smadzeņu procesiem: neiroiekaisumu, sinapšu blīvuma samazināšanos un acetilholīnesterāzes aktivitāti. Pētījuma rezultāti parāda, ka abās devās metformīns spēj novērst mikrogliozi, sinaptiskā blīvuma samazināšanos un normalizēt acetilholīnesterāzes blīvumu modeļžurku smadzenēs. Iegūtie dati norāda uz metformīna neiroprotektīvo potenciālu sporādiskās Alcheimera slimības patoloģisko izmaiņu novēršanu. Nepieciešami tālākie pētījumi, lai uzzinātu visu metformīna neiroprotektīva potenciāla klāstu sAD-tipa modelī.
Mechanical activity of small and large intestine in normal and mdx mice: a comparative analysis.
1999
The aim of this study was to compare the motor pattern (recorded as changes in intraluminal pressure) of isolated duodenum and proximal colon between dystrophic mdx and normal mice. When duodenal recordings from control preparations were compared with mdx mice there was no significant difference in the spontaneous motor pattern, responses to electrical nerve stimulation or sensitivity to pharmacological agents. Colonic segments from mdx mice showed a more complex motor pattern, consisting of contractions with amplitude and frequency similar to those of controls and by additional contractions with lower amplitude and higher frequency. Moreover, 70% of the colonic preparations from mdx mice d…
Duodenal contractile activity in dystrophic (mdx) mice: reduction of nitric oxide influence.
2003
The present study was undertaken to analyse duodenal contractility in adult dystrophic (mdx) mice. The spontaneous changes of the isometric tension and the responses of longitudinal duodenal muscle to nonadrenergic, noncholinergic (NANC) nerve stimulation and to exogenous drugs were compared between normal and mdx mice. Duodenal segments from mdx mice displayed spontaneous contractions with higher frequency than normals. N omega-nitro-L-arginine methyl ester (L-NAME) increased the frequency of contractions in normals without affecting that in mdx mice. In normals, NANC nerve stimulation elicited a transient relaxation abolished by L-NAME. In mdx mice a frank relaxation was not observed, the…
Endocannabinoid Role in Synaptic Plasticity and Learning
2009
Endocannabinoids have recently emerged as versatile modulators of synaptic transmission and can act as retrograde neurotransmitters. As they cannot be stored in synaptic vesicles, endocannabinoid signaling is believed to start ‘on-demand,’ via a stimulus-dependent synthesis from membranous precursors at the postsynaptic site. After synthesis, endocannabinoids bind presynaptically to cannabinoid type 1 (CB1) receptors, leading to a short- or long-term suppression of neurotransmitter release. CB1 receptors are present in a plethora of different synaptic connections in the brain. Electrophysiological and behavioral analyses of mutant mice lacking CB1 receptors and of pharmacologically treated …
La Cappella Musicale della Chiesa Madre di Enna: pratiche musicali e continuità stilistica fra 1700 e 1900
2006
Musica nel "Poliorama Pittoresco"
2010
Il saggio rintraccia ed analizza i contributi a carattere musicale comparsi nella rivista napoletana "Poliorama pittoresco", uscita dal 1836 al 1860. Accanto a profili biografici e ad articoli encomiastici (specialmente su Rossini), la rivista pubblica interessanti articoli dedicati alla musica popolare. In particolare nel saggio vengono analizzati due contributi sui Viggianesi, musicisti ambulanti giunti dalla Basilicata in tutta Europa ed anche in America, e un importante testo di Giuseppe Regaldi del 1847 dedicato alla canzone napoletana, ricco di preziose informazioni di prima mano (tale testo è riportato per intero in appendice).