Search results for "nematoda"
showing 10 items of 39 documents
Seasonal trend of Anisakidae infestation in South Mediterranean bluefish
2020
A total of 1104 fish samples from markets of Sicily were analysed for the detection and species identification of Anisakidae nematodes. The preliminary analysis of the fish samples showed the presence of 2459 larvae. All the fish species revealed different prevalence of infestation, with a maximum of 100% for Lepidopus caudatus and a minimum of 4.5% in Sardina pilchardus. The 80% of the larvae examined by PCR-RFLP analysis belonged to Anisakis pegreffii species. The seasonal infestation trend of Anisakis was evaluated in all the fish sample examined. The results of the seasonal infestation trend showed a marked connection with the ecological aspects of the fish species examined. As far as w…
Raphidascaris (Raphidascaris) macrouri n. sp. (Nematoda: Anisakidae) from two deep-sea macrourid fishes in the Western Mediterranean: Morphological a…
2015
Abstract A new nematode species, Raphidascaris ( Raphidascaris ) macrouri n. sp. (Anisakidae), is described from male and female specimens found in the intestine, and occasionally in stomach and pyloric caeca, of two deep-water macrourid fishes (Gadiformes) off Barcelona, Mediterranean Sea: Nezumia aequalis (Gunther) (type-host) and Trachyrincus scabrus (Rafinesque). Based on light and scanning electron microscopy examination, the new species shows similar morphological features as the other four valid species of the subgenus Raphidascaris Railliet & Henry, 1915, but it differs from Raphidascaris ( Raphidascaris ) acus (Bloch, 1779), Raphidascaris (Raphidascaris ) lutjani Olsen, 1952 and Ra…
Mitochondrial genome sequence and gene order of Sipunculus nudus give additional support for an inclusion of Sipuncula into Annelida.
2010
Abstract Background Mitochondrial genomes are a valuable source of data for analysing phylogenetic relationships. Besides sequence information, mitochondrial gene order may add phylogenetically useful information, too. Sipuncula are unsegmented marine worms, traditionally placed in their own phylum. Recent molecular and morphological findings suggest a close affinity to the segmented Annelida. Results The first complete mitochondrial genome of a member of Sipuncula, Sipunculus nudus, is presented. All 37 genes characteristic for metazoan mtDNA were detected and are encoded on the same strand. The mitochondrial gene order (protein-coding and ribosomal RNA genes) resembles that of annelids, b…
Respiratory proteins in Sipunculus nudus--implications for phylogeny and evolution of the hemerythrin family.
2009
Three major classes of respiratory proteins are known, hemoglobin, molluscan and arthropod hemocyanin, and hemerythrin (Hr). Similar to hemoglobin, respiratory Hr is packed into erythrocytes floating in the coelomic fluid and is only known from sipunculids, brachiopods, and priapulids. Owing to this scattered distribution, the presence of Hr is generally assumed to be the plesiomorphic condition without phylogenetic importance. By sequencing 2000 Expressed Sequence Tags (ESTs) from Sipunculus nudus, we found 75 Hr-coding ESTs assembled to 20 cDNA contigs classified as four distinct Hr isoforms: three polymeric Hrs (subunit A, A', and B) and the monomeric myo-hemerythrin (myoHr). Phylogeneti…
Helminth infracommunities of a population of the Gran Canaria giant lizard Gallotia stehlini
2004
AbstractA survey of gastro-intestinal helminth communities of Gallotia stehlini (Sauria: Lacertidae) from Gran Canaria island (Canary Archipelago, Spain), was conducted to determine the prevalence, abundance and species diversity of intestinal parasites in these lizards. Pharyngodonid nematodes were the most common intestinal helminths, three species being Gallotia specialists. Helminth infracommunities of G. stehlini were rich and appear to be closer to the interactive end of the continuum isolationist–interactive helminth communities, according to the high values of helminth diversity. It is the first case of a saurian reptile showing this kind of diverse helminth infracommunity, produced…
Análisis de la interacciones parásito-hospedador en el modelo Echinostoma caproni-roedor, con énfasis en los factores que determinan el curso de la i…
2015
En la presente Tesis Doctoral se analizan diferentes parámetros implicados en las relaciones parásito-hospedador en helmintiasis intestinales mediante la utilización del modelo experimental Echinostoma caproni-roedor. Con esta finalidad se estudian diversos aspectos inmunológicos, además de las alteraciones intestinales inducidas por la infección en dos sistemas parásito-hospedador: E. caproni-ratón y E. caproni-rata. Los resultados obtenidos ponen de manifiesto que el IFN-γ y el óxido nítrico son mediadores fundamentales en el desarrollo de infecciones crónicas en ratón, induciendo disfunción mitocondrial en las células del epitelio intestinal e hiperplasia tisular. No obstante, se sugiere…
Biodiversity of nematofauna associated with the rice field agro-ecosystem in north Italy
2008
This research is intended to give an idea on the soil biodiversity associated with the rice field agro-ecosystem. The research was carried out in two areas of North Italy where Oryza sativa is a traditional crop. The nematodes were extracted from soil samples and from the rhizosphere of rice plants. The nematode community was investigated for the genus composition, trophic structure and biodiversity. 22 families and 45 different genera were recorded. The dominant trophic group was the bacteriophagous with the genus Rhabditis. Other important genera were Pratylenchus, Helicotylenchus and Acrobeloides. Phytophagous represent in almost all fields not more than the 20% of the total nematodes. I…
Coupling geochemical, mineralogical and microbiological approaches to assess the health of contaminated soil around the Almalyk mining and smelter co…
2013
This study describes the impact of airborne pollution resulting from mining and smelting activities on the soils of the Almalyk mining and industrial area (NE Uzbekistan). Samples were collected along a transect downwind of the industrial area. Enriched contents of some metals were found in the upper soil layers near the metallurgical complex (Zn≤3010 mg kg(-1), Pb≤630 mg kg(-1), Cd≤30 mg kg(-1)) which suggests that these metals were derived from local stack emissions. The morphology and internal microstructure of metal-bearing spherical particles found in the heavy mineral fraction suggest that these particles were probably a result of inefficient flue gas cleaning technique of the smelter…
Proteomic analysis of the pinworm Syphacia muris (Nematoda: Oxyuridae), a parasite of laboratory rats
2012
Syphacia muris (Nematoda: Oxyuridae) is a ubiquitous nematode that commonly infects rats in the laboratory which can interfere in the development of biological assays. The somatic extract of S. muris adults collected from infected rats was investigated using a proteomic approach. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 359 proteins were accurately identified from the worms. The largest protein families consisted of metabolic enzymes and those involved in the nucleic metabolism and cell cycle. Proteins of transmembrane…
CiliaCarta: An integrated and validated compendium of ciliary genes
2019
The cilium is an essential organelle at the surface of mammalian cells whose dysfunction causes a wide range of genetic diseases collectively called ciliopathies. The current rate at which new ciliopathy genes are identified suggests that many ciliary components remain undiscovered. We generated and rigorously analyzed genomic, proteomic, transcriptomic and evolutionary data and systematically integrated these using Bayesian statistics into a predictive score for ciliary function. This resulted in 285 candidate ciliary genes. We generated independent experimental evidence of ciliary associations for 24 out of 36 analyzed candidate proteins using multiple cell and animal model systems (mouse…