Search results for "neutralization"
showing 9 items of 59 documents
Short synthetic CDR-peptides forming the antibody combining site of the monoclonal antibody against RNA bacteriophage fr neutralize the phage activit…
1996
The construction of a mouse hybridoma FRS2 secreting neutralizing monoclonal antibody specific for RNA bacteriophages fr, MS2 and GA is reported. The genes encoding the variable domains of the monoclonal antibody FRS2 heavy and light chains were cloned and sequenced and the corresponding complementarity determining region (CDR) peptides were chemically synthesized. The CDR-peptides were tested for their ability to neutralize the activity of RNA phage fr and related RNA phages MS2 and GA. The CDR-derived peptides H2, L2 and L3 interacted with the fr phage particles and neutralized fr phage activity. Two of these peptides-H2 and L3 also had the ability to neutralize partly the activity of rel…
The role of insulin-like growth factor II in the malignant transformation of rat liver oval cells
1997
Oval cells are small nonparenchymal epithelial cells that first appear in the periportal areas of the liver and thereafter invade the whole parenchyma when mice or rats are exposed to a variety of chemical carcinogens. In the present study we have analyzed the expression of insulin-like growth factor II (IGF II) in the recently established oval cell line OC/CDE 22 and its malignantly transformed counterpart (the M22 cells) and the biological consequences of the constitutive expression of IGF II in oval cells. OC/CDE 22 cells do not express the above-mentioned growth factor, whereas the M22 cells do and addition of a neutralizing anti-IGF II antibody to M22 cells resulted in an almost comple…
Induction of Type-Specific Neutralizing Antibodies by Capsomeres of Human Papillomavirus Type 33
2001
Abstract The immunogenicity of capsomeres of human papillomavirus type 33 was evaluated in a dose–response analysis. Capsomeres were obtained free of capsids by expression of L1 carrying the single point mutation C427S. Neutralizing antibodies were detected using an in vitro pseudoinfection assay. Capsomeres induced type-specific, neutralizing antibodies in mice even in the absence of adjuvant. The neutralization titers of immune sera raised without adjuvant were 10- to 20-fold lower than those of antisera to virus-like particles, but virtually identical using Freund's adjuvant. These data indicate that capsomeres may substitute for virus-like particles in future vaccines when used with an …
Music Piracy Neutralization and the Youth of the 2010's
2014
In this qualitative research, music piracy among the youth was studied by applying Sykes’ and Matza’s neutralization theory. The key objective of the study was to find out how youths view music piracy, and how they give accounts for it using techniques of neutralization. According to the conducted semi-structured interviews, youths do acknowledge the ethical and economic problems of music piracy. However, piracy is still exercised, and in order to justify this, multiple neutralization techniques are used. The most characteristic of the employed techniques was “claim of normalcy”, with “denial of victim” and “justification by comparison” also appearing frequently. In order to prevent piracy,…
Dissonance and Neutralization of Subscription Streaming Era Digital Music Piracy : An Initial Exploration
2016
Both legal and illegal forms of digital music consumption continue to evolve with wider adoption of subscription streaming services. With this paper, we aim to extend theory on digital music piracy by showing that the rising controversy and diminishing acceptance of illegal forms of consumption call for new theoretical components and interactions. We introduce a model that integrates insights from neutralization and cognitive dissonance theories. As an initial empirical test of the proposed Dissonance-Neutralization model, we estimate a structural equation model based on self-administered survey data obtained from 322 respondents. Our results uncover potential demographic differences in pir…
Zapobieganie stosowania technik neutralizacji jako zadanie wychowawcze
2017
Autorka w niniejszym artykule zwraca uwagę na zjawisko i mechanizmy usprawiedliwiania własnego zachowania z perspektywy psychologii poznawczej, psychologii społecznej i koncepcji kryminologicznej Sykesa i Matzy. Usprawiedliwianie własnego działania, jeśli skutecznie neutralizuje uczucia moralne, utrudnia jednostce uczenie się na własnych błędach, co prowadzi do powtarzania zachowań niezgodnych z normami społecznymi i prawnymi. To prosta droga do przestępczości. Dlatego rolą pedagogów je s t niedopuszczenie by dzieci i młodzież uczyli się sprawnych technik neutralizacji.
Revisiting neutralization theory and its underlying assumptions to inspire future information security research
2022
Over two decades ago, neutralization theory was introduced to information systems research from the field of criminology and is currently emerging as an influential foundation to both explain and solve the information security policy noncompliance problem. Much of what we know about the theory focuses exclusively on the neutralization techniques identified in the original as well as subsequent criminological writings. What is often left unexamined in IS research is the underlying assumptions about the theory’s core elements; assumptions about the actor, the act, the normative system, and the nature of neutralizing itself. The objective of this commentary is to revisit the origin of neutrali…
Generation and Applications of HPV Pseudovirions Using Vaccinia Virus
2005
This chapter outlines the generation and application of human papillomavirus type 33 (HPV 33) pseudovirions. These pseudovirions are structurally indistinguishable from native virions and are therefore valuable tools for the study of papillomavirus/cell interactions. The method describes (1) the construction of vaccinia viruses recombinant for the major and minor HPV capsid proteins, L1 and L2, respectively, (2) the transfection of Cos7 cells with a marker plasmid replicating to high copy numbers, (3) the expression of L1 and L2 using the vaccinia virus expression system, (4) the extraction, purification, and analysis of HPV-33 pseudovirions, (5) pseudoinfection assays, (6) pre- and post-at…
Generation and neutralization of pseudovirions of human papillomavirus type 33
1997
Since human papillomaviruses (HPV) cannot be propagated in cell culture, the generation of infectious virions in vitro is a highly desirable goal. Here we report that pseudovirions can be generated by the assembly of virus-like particles (VLPs) in COS-7 cells containing multiple copies of a marker plasmid. Using recombinant vaccinia viruses, we have obtained spherical VLPs of HPV type 33 (HPV-33) which fractionate into heavy and light VLPs in cesium chloride density gradients. VLPs in the heavy fraction (1.31 g/cm3) carry the plasmid in DNase-resistant form and are capable of transferring the genetic marker located on the plasmid to COS-7 cells in a DNase-resistant way (pseudoinfection). Th…