Search results for "neutrophils"

showing 10 items of 316 documents

Patterns of Inflammatory Responses in Large and Small Airways in Smokers with and without Chronic Obstructive Pulmonary Disease

2010

<i>Background:</i> Chronic obstructive pulmonary disease (COPD) is characterised by progressive and irreversible airway obstruction. Smoking causes persistent inflammation in lung tissue. However, differences in inflammatory responses between the large and small airways have not been systematically explored among smokers with and without COPD. <i>Objectives:</i> The aim of our research was to characterise the expression and localisation of NF-ĸBp65 and histone deacetylase 2 (HDAC2) as well as inflammatory cell (macrophages, lymphocytes, neutrophils) distribution in large and small airways, in nonsmokers and in smokers with and without COPD. <i>Methods:</i&gt…

MalePulmonary and Respiratory Medicinemedicine.medical_specialtyNeutrophilsHistone Deacetylase 2Pulmonary diseaseBronchiCell CountInflammationCD8-Positive T-Lymphocytesurologic and male genital diseasesPersistent inflammationPulmonary Disease Chronic ObstructiveRisk FactorsCarcinoma Non-Small-Cell LungInternal medicineMacrophages AlveolarCarcinomamedicineHumansLungAgedCOPDSmall airwaysbusiness.industrySmokingRespiratory diseaseTranscription Factor RelAMiddle Agedrespiratory systemAirway obstructionmedicine.diseaseImmunohistochemistryrespiratory tract diseasesImmunologyDisease ProgressionCardiologyFemalemedicine.symptombusinessRespiration
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In vitro anti-inflammatory effects of AZD8999, a novel bifunctional muscarinic acetylcholine receptor antagonist /β2-adrenoceptor agonist (MABA) comp…

2019

Recent evidence indicates that AZD8999 (LAS190792), a novel muscarinic acetylcholine receptor antagonist and β2-adrenoceptor agonist (MABA) in development for chronic respiratory diseases, induces potent and sustained relaxant effects in human bronchi by adressing both muscarinic acetylcholine receptors and β2-adrenoceptor. However, the anti-inflammatory effects of the AZD8999 monotherapy or in combination with corticosteroids are unknown. This study investigates the anti-inflammatory effects of AZD8999 in monotherapy and combined with fluticasone propionate in neutrophils from healthy and chronic obstructive pulmonary disease (COPD) patients. Peripheral blood neutrophils from healthy and C…

MalePulmonologyNeutrophilsPhysiologyAnti-Inflammatory AgentsPharmacologyPathology and Laboratory MedicineBiochemistryPulmonary Disease Chronic ObstructiveWhite Blood CellsGlucocorticoid receptorAnimal CellsMuscarinic acetylcholine receptorMedicine and Health SciencesPost-Translational ModificationPhosphorylationReceptorImmune ResponseMultidisciplinaryPharmaceuticsQRDrug SynergismMiddle AgedReceptors MuscarinicHealthy VolunteersBody FluidsChemistryBloodPhysical SciencesQuinolinesMedicineDrug Therapy CombinationFemalemedicine.symptomCellular TypesAnatomymedicine.drugResearch ArticleSignal TransductionAgonistTransmembrane Receptorsmedicine.drug_classp38 mitogen-activated protein kinasesChronic Obstructive Pulmonary DiseaseImmune CellsScienceImmunologyInflammationMuscarinic AntagonistsThiophenesFluticasone propionateSigns and SymptomsDrug TherapyCyclohexanesDiagnostic MedicinemedicineHumansAdrenergic beta-2 Receptor AgonistsAgedInflammationBlood CellsDose-Response Relationship Drugbusiness.industryAntagonistChemical CompoundsBiology and Life SciencesProteinsCell BiologyAcetylcholine ReceptorsFluticasoneMuscarinic Acetylcholine ReceptorsReceptors Adrenergic beta-2PropionatesbusinessReceptor Antagonist TherapyPLoS ONE
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Co-option of Neutrophil Fates by Tissue Environments

2020

Classically considered short-lived and purely defensive leukocytes, neutrophils are unique in their fast and moldable response to stimulation. This plastic behavior may underlie variable and even antagonistic functions during inflammation or cancer, yet the full spectrum of neutrophil properties as they enter healthy tissues remains unexplored. Using a new model to track neutrophil fates, we found short but variable lifetimes across multiple tissues. Through analysis of the receptor, transcriptional, and chromatin accessibility landscapes, we identify varying neutrophil states and assign non-canonical functions, including vascular repair and hematopoietic homeostasis. Accordingly, depletion…

MaleReceptors CXCR4Transcription GeneticAngiogenesisNeutrophilsMedizinNeovascularization PhysiologicInflammationBiologyCXCR4General Biochemistry Genetics and Molecular Biology03 medical and health sciences0302 clinical medicineSingle-cell analysismedicineNuclear Receptor Subfamily 4 Group A Member 1AnimalsCell LineageReceptorLung030304 developmental biology0303 health sciencesInnate immune systemChromatinChromatinCell biologyHematopoiesisIntestinesMice Inbred C57BLOrgan SpecificityFemalemedicine.symptomSingle-Cell AnalysisTranscriptome030217 neurology & neurosurgeryHomeostasisCell
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Convergent sets of data from in vivo and in vitro methods point to an active role of Hsp60 in chronic obstructive pulmonary disease pathogenesis.

2011

BackgroundIt is increasingly clear that some heat shock proteins (Hsps) play a role in inflammation. Here, we report results showing participation of Hsp60 in the pathogenesis of chronic obstructive pulmonary diseases (COPD), as indicated by data from both in vivo and in vitro analyses.Methods and resultsBronchial biopsies from patients with stable COPD, smoker controls with normal lung function, and non-smoker controls were studied. We quantified by immunohistochemistry levels of Hsp10, Hsp27, Hsp40, Hsp60, Hsp70, Hsp90, and HSF-1, along with levels of inflammatory markers. Hsp10, Hsp40, and Hsp60 were increased during progression of disease. We found also a positive correlation between th…

MaleSTRESSPulmonologyChronic Obstructive Pulmonary DiseasesNeutrophilsBiopsyGene ExpressionCD8-Positive T-Lymphocytesmedicine.disease_causeBiochemistryEpitheliumPulmonary function testingPathogenesisACTIVATIONPulmonary Disease Chronic ObstructiveMolecular Cell BiologyLungCOPDMultidisciplinaryReverse Transcriptase Polymerase Chain ReactionCOPD Hsp60QRCOPD heat shock proteins inflammationMiddle AgedImmunohistochemistrymedicine.anatomical_structureEXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITISMedicineFemalemedicine.symptomInflammation MediatorsSPINAL-CORDResearch ArticleEXPRESSIONanimal structuresCOPD; heat shock proteins; inflammationScienceImmunologyMolecular Sequence DataInflammationBronchichemical and pharmacologic phenomenaHEAT-SHOCK-PROTEIN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ACUTE LUNG INJURY SPINAL-CORD CELL-DEATH KAPPA-B HEAT-SHOCK-PROTEIN-60 STRESS EXPRESSION ACTIVATIONKAPPA-BBiologyHEAT-SHOCK-PROTEINMicrobiologycomplex mixturesCell LineACUTE LUNG INJURYMolecular GeneticsIn vivoStress PhysiologicalHeat shock proteinmedicineGeneticsHumansCOPDRNA MessengerBiologyAgedLungMucous MembraneBase SequenceSettore BIO/16 - Anatomia UmanaMacrophagesfungiImmunityTranscription Factor RelAProteinsComputational BiologyChaperonin 60medicine.diseaseChaperone Proteinsrespiratory tract diseasesGene Expression RegulationCELL-DEATHHEAT-SHOCK-PROTEIN-60inflammationImmunologyheat shock proteinsClinical ImmunologyOxidative stressBiomarkers
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Two‐dimensional analysis of myocardial protein expression following myocardial ischemia and reperfusion in rabbits

2002

Myocardial ischemia and reperfusion injury (MI/R) can be related to leukocyte activation with subsequent release of cytokines and oxygen derived free radicals. Activation of the complement system has been implicated in the pathogenesis of myocardial ischemia and reperfusion injury. Inflammatory injury will subsequently result in cellular activation and protein synthesis. In the present study we analyzed the myocardial protein expression and its pattern following myocardial ischemia and reperfusion, with and without complement inhibition with the synthetic serine protease inhibitor Futhan/nafamstat mesilate (FUT-175) known to inhibit classical and alternative complement pathway in a rabbit m…

MaleSerine Proteinase InhibitorsNecrosisProteomeNeutrophilsMyocardial IschemiaIschemiaMyocardial Reperfusion InjuryPharmacologyGuanidinesBiochemistrySuperoxide dismutaseNecrosisRandom AllocationComplement inhibitormedicineAnimalsElectrophoresis Gel Two-DimensionalCreatine KinaseMolecular BiologybiologySuperoxide DismutaseChemistryGene Expression ProfilingMyocardiumHemodynamicsProteinsalpha-Crystallin B Chainmedicine.diseaseBenzamidinesComplement systemBiochemistrybiology.proteinAlternative complement pathwayCreatine kinaseRabbitsmedicine.symptomReperfusion injuryPROTEOMICS
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Inhibition of phospholipase A2 activities and some inflammatory responses by the marine product ircinin

1996

The marine product ircinin has been tested for its effects on secretory and cytosolic phospholipase A2 (PLA2) activities in vitro as well as for inhibition of cellular functions in human neutrophils and inflammatory responses in mice. Ircinin inhibited Naja naja venom, human synovial recombinant, bee venom and zymosan-injected rat air pouch PLA2 with IC50 values in the microM range, similar to those of the known inhibitor scalaradial. On the other hand, ircinin was less active on cytosolic PLA2 from human monocytes and decreased potently the release of LTB4 in human neutrophils. This marine product affected weakly human neutrophil functions like superoxide generation and degranulation. In t…

MaleSesterterpenesNeutrophilsAnti-Inflammatory AgentsInflammationPharmacologyPhospholipases AMicechemistry.chemical_compoundPhospholipase A2SuperoxidesIn vivomedicineAnimalsEdemaHumansPharmacologyAnalysis of VarianceDose-Response Relationship DrugbiologyTerpenesSuperoxideDegranulationGeneral MedicineLeukotriene A4In vitroPoriferaRatsPhospholipases A2CytosolchemistryBiochemistryMyeloperoxidasebiology.proteinHomosteroidsMarine Toxinslipids (amino acids peptides and proteins)medicine.symptomLeukocyte ElastaseNaunyn-Schmiedeberg's Archives of Pharmacology
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White blood cell counts as risk markers of developing metabolic syndrome and its components in the Predimed study.

2013

Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; TRIAL REGISTRATION Controlled-Trials.comISRCTN35739639. BACKGROUND The Metabolic Syndrome (MetS) is a cluster of metabolic abnormalities that includes hyperglucemia, hypertension, dyslipidemia and central obesity, conferring an increased risk of cardiovascular disease. The white blood cell (WBC) count has been proposed as a marker for predicting cardiovascular risk. However, few prospective studies have evaluated the relationship between WBC subtypes and risk of MetS. METHODS Participants were recruited from seven PREDIMED study centers. Both a baseline cross-sectional (n = 4,377) and a prospe…

MaleSíndrome metabòlicaMediterranean dietNeutrophilsEpidemiologyEstudios transversalesMetabolic disorders:Named Groups::Persons::Age Groups::Adult::Aged::Aged 80 and over [Medical Subject Headings]Disease:Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]Diet Mediterranean:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Risk::Risk Factors [Medical Subject Headings]:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Leukocyte CountLymphocitesRisk FactorsBlood plasmaPathologyMedicineProspective StudiesProspective cohort studyAged 80 and overMetabolic SyndromeClinical ChemistryMultidisciplinary:Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Nutrition Therapy::Diet Therapy::Diet Mediterranean [Medical Subject Headings]QRMiddle AgedMetabolic syndromeClinical Laboratory Sciences1932-6203:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings]medicine.anatomical_structureCholesterolCardiovascular diseasesCardiovascular DiseasesMedicineWhite blood cellsFemalePublic HealthEnfermedades cardiovascularesResearch Articlemedicine.medical_specialty:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies [Medical Subject Headings]Recuento de linfocitosScience:Check Tags::Male [Medical Subject Headings]Mediterranean cookingDiagnostic MedicineInternal medicineWhite blood cell:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Count::Blood Cell Count::Leukocyte Count::Lymphocyte Count [Medical Subject Headings]Cuina mediterràniaHumansLymphocyte CountObesity:Named Groups::Persons::Age Groups::Adult::Aged [Medical Subject Headings]:Diseases::Cardiovascular Diseases [Medical Subject Headings]Cardiovascular Disease EpidemiologyAged:Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Metabolic Syndrome X [Medical Subject Headings]business.industryMalalties cardiovascularsRecuento de leucocitosSíndrome X metabólicomedicine.diseaseObesityLong-Term CareDieta mediterránea:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Count::Blood Cell Count::Leukocyte Count [Medical Subject Headings]Biomarker EpidemiologyCross-Sectional Studies:Check Tags::Female [Medical Subject Headings]GeriatricsImmunologyClinical ImmunologyPreventive MedicineMetabolic syndrome:Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils [Medical Subject Headings]businessDyslipidemiaBiomarkersGeneral Pathology
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Effects of exercise training on airway responsiveness and airway cells in healthy subjects.

2010

J Appl Physiol. 2010 Aug;109(2):288-94. Epub 2010 Jun 10. Effects of exercise training on airway responsiveness and airway cells in healthy subjects. Scichilone N, Morici G, Zangla D, Chimenti L, Davì E, Reitano S, Paternò A, Santagata R, Togias A, Bellia V, Bonsignore MR. SourceDept. of Internal Medicine, Div. of Pulmonology (DIBIMIS Univ. of Palermo, "Villa Sofia-Cervello" Hospital, Via Trabucco 180, 90146 Palermo, Italy. n.scichilone@libero.it Abstract Airway responsiveness to methacholine (Mch) in the absence of deep inspirations (DIs) is lower in athletes compared with sedentary individuals. In this prospective study, we tested the hypothesis that a training exercise program reduces th…

MaleTime FactorsFunctional Residual CapacityPhysiologyVital CapacitySettore BIO/09 - FisiologiaBronchoconstrictor Agentsneutrophilsrowing; methacoline; neutrophilsForced Expiratory VolumeUteroglobinProspective StudiesRespiratory systemProspective cohort studyLungMethacholine Chloriderowingbiologyrespiratory systemResidual Volumemedicine.anatomical_structureInhalationAnesthesiaBronchial HyperreactivityInflammation MediatorsAirway responsivenessmedicine.drugAdultmedicine.medical_specialtyBronchoconstrictionPhysical exercisemethacolineSettore MED/10 - Malattie Dell'Apparato RespiratorioBronchial Provocation TestsYoung AdultPhysiology (medical)medicineHumansMuscle StrengthExerciseAthletesbusiness.industryInterleukin-8Total Lung CapacitySputumbiology.organism_classificationSpirometryPhysical therapyMethacholineSedentary BehaviorbusinessAirwayRespiratory tractJournal of applied physiology (Bethesda, Md. : 1985)
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Sudden sensorineural hearing loss: is there a relationship between routine haematological parameters and audiogram shapes?

2016

Objective: To investigate the relationship between haematological routine parameters and audiogram shapes in patients affected by sudden sensorineural hearing loss (SSNHL). Design: A retrospective study. All patients were divided into four groups according to the audiometric curve and mean values of haematological parameters (haemoglobin, white blood cell, neutrophils and lymphocytes relative count, platelet count, haematocrit, prothrombin time, activated partial thromboplastin time, fibrinogen and neutrophil-to-lymphocite ratio) of each group were statistically compared. The prognostic role of blood profile and coagulation test was also examined. Study sample: A cohort of 183 SSNHL patient…

MaleTime FactorsNeutrophilshaematological parameterAudiologyLanguage and LinguisticsHemoglobinsLeukocyte Count0302 clinical medicineHearingLymphocytes030223 otorhinolaryngologyHematologic Testsmedicine.diagnostic_testComplete blood countAudiogramSudden sensorineural hearing lossMiddle AgedSettore MED/32 - AudiologiaTreatment OutcomeSettore MED/31 - Otorinolaringoiatriamedicine.anatomical_structureHematocrit030220 oncology & carcinogenesisCohortAudiometry Pure-ToneFemalePartial Thromboplastin TimeSteroidsmedicine.symptomPartial thromboplastin timeAdultBlood PlateletsLinguistics and Languagemedicine.medical_specialtyHearing lossHearing Loss Sensorineural03 medical and health sciencesSpeech and HearingAudiogram shapePredictive Value of TestsWhite blood cellmedicineHumansBlood CoagulationRetrospective StudiesProthrombin timePlatelet Countbusiness.industryFibrinogenAuditory ThresholdRetrospective cohort studyRecovery of FunctionHearing Loss Suddenaudiogram shape; haematological parameters; Sudden sensorineural hearing lossProthrombin TimebusinessBiomarkersInternational Journal of Audiology
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Prediction of leukocyte counts during paediatric acute lymphoblastic leukaemia maintenance therapy

2019

Maintenance chemotherapy with oral 6-mercaptopurine and methotrexate remains a cornerstone of modern therapy for acute lymphoblastic leukaemia. The dosage and intensity of therapy are based on surrogate markers such as peripheral blood leukocyte and neutrophil counts. Dosage based leukocyte count predictions could provide support for dosage decisions clinicians face trying to find and maintain an appropriate dosage for the individual patient. We present two Bayesian nonlinear state space models for predicting patient leukocyte counts during the maintenance therapy. The models simplify some aspects of previously proposed models but allow for some extra flexibility. Our second model is an ext…

MaleTime seriesAdolescentaikasarjatNeutrophilsDatasets as Topiclcsh:MedicinebiomarkkeritModels BiologicalArticleMaintenance ChemotherapyPaediatric cancerLeukocyte CountSyöpätaudit - CancersAntineoplastic Combined Chemotherapy ProtocolsLeukocytesHumansDrug Dosage CalculationsChildlcsh:Sciencetilastolliset mallitStochastic modellingstokastiset prosessitStochastic ProcessesvalkosolutMercaptopurinebayesilainen menetelmäStatisticslcsh:RInfantennusteetBayes TheoremPrecursor Cell Lymphoblastic Leukemia-LymphomaApplied mathematicsMethotrexateChild Preschoollääkehoitoakuutti lymfaattinen leukemiasyöpätauditFemalelcsh:Q
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