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showing 10 items of 6290 documents

Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants

2018

Abstract As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel, 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. The European Commission has mandated EFSA to develop a technical note to the applicants on, and checking of, the quality of the methodology, analysis and reporting covering complete sequencing of the insert and flanking regions, insertion site analysis of the GM event, and generational stability and integrity. This Technical Note puts together requirements and r…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]2405 Parasitologymolecularcharacterisationnext‐generation sequencingContext (language use)Plant ScienceComputational biologyGenetically modified cropsBiology01 natural sciencesMicrobiologyGenomeInsert (molecular biology)DNA sequencing03 medical and health sciencessymbols.namesake1110 Plant Sciencegenetically modified organismgenetic stabilityDNA sequencing1106 Food ScienceSanger sequencinggenetically modified organisms2404 Microbiologyrisk assessmentmolecular characterisation10079 Institute of Veterinary Pharmacology and ToxicologyGenetically modified organism3401 Veterinary (miscellaneous)Scientific Opinion030104 developmental biologyNGSsymbols570 Life sciences; biologyAnimal Science and ZoologyParasitologynext-generation sequencing1103 Animal Science and Zoology010606 plant biology & botanyFood ScienceVerification and validation
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Assessment of genetically modified cotton GHB614 × LLCotton25 × MON 15985 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFS…

2018

The three-event stack cotton GHB614 x LLCotton25 x MON 15985 was produced by conventional crossing to combine three single cotton events, GHB614, LLCotton25 and MON 15985. The EFSA GMO Panel previously assessed the three single events and did not identify safety concerns. No new data on the single events that could lead to modification of the original conclusions on their safety were identified. Based on the molecular, agronomic, phenotypic and compositional characteristics, the combination of the single events and of the newly expressed proteins in the three-event stack cotton did not give rise to food and feed safety or nutritional issues. Food and feed derived from cotton GHB614 x LLCott…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]Plant ScienceTP1-1185Biology01 natural sciencesMicrobiology03 medical and health sciencesEnvironmental safetyNPTIIGUSCry1AcTX341-641cotton GHB614 × LLCotton25 × MON 15985Cry2Ab2cotton GHB614 x LLCotton25 x MON 159852. Zero hungerAnimal healthbusiness.industryNutrition. Foods and food supplyGMOChemical technologyRegulation (EC) 1829/2003Food safetyGenetically modified organismBiotechnologySettore AGR/02 - Agronomia E Coltivazioni ErbaceeScientific Opinion030104 developmental biologySettore AGR/11 - Entomologia Generale E ApplicataAnimal Science and ZoologyParasitologyGMO; cotton GHB614 x LLCotton25 x MON 15985; Regulation (EC) 1829/2003; Cry1Ac; Cry2Ab2; GUS; NPTII; 2mEPSPS; PATbusiness2mEPSPSPAT010606 plant biology & botanyFood Science
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Assessment of genetically modified maize MON 87403 for food and feed uses, import and processing, under Regulation (EC) No 1829/2003 (application EFS…

2018

GMO; maize (Zea mays); MON 87403; ear biomass; Regulation (EC) No 1829/2003; International audience; aize MON 87403 was developed to increase ear biomass at early reproductive phase through the expression of a modified AtHB17 gene from Arabidopsis thaliana, encoding a plant transcription factor of the HD-Zip II family. The molecular characterisation data and bioinformatic analyses did not identify issues requiring assessment for food and feed safety. No statistically significant differences in the agronomic and phenotypic characteristics tested between maize MON 87403 and its conventional counterpart were identified. The compositional analysis of maize MON 87403 did not identify differences…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]Plant ScienceTP1-1185GMO; maize (Zea mays); MON 87403; ear biomass; Regulation (EC) No 1829/2003Biologyear biomass01 natural sciencesMicrobiologyMON 87403[SHS]Humanities and Social Sciences03 medical and health sciencesEnvironmental safetyear bioma[SDV.IDA]Life Sciences [q-bio]/Food engineering[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyTX341-641MON 87403[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineeringmaize (Zea mays)2. Zero hungerGenetically modified maizeAnimal healthbusiness.industryNutrition. Foods and food supplyGMOChemical technologyfungiRegulation (EC) No 1829/2003maize (Zea mays)Food safetyBiotechnologySettore AGR/02 - Agronomia E Coltivazioni Erbacee030104 developmental biologyScientific OpinionSettore AGR/11 - Entomologia Generale E Applicata[SDE]Environmental SciencesAnimal Science and ZoologyParasitologybusiness010606 plant biology & botanyFood ScienceRegulation (EC) No 1829/2003
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Updated pest categorisation of Xylella fastidiosa

2018

Abstract Following a request from the European Commission, the EFSA Plant Health Panel updated its pest categorisation of Xylella fastidiosa, previously delivered as part of the pest risk assessment published in 2015. X. fastidiosa is a Gram‐negative bacterium, responsible for various plant diseases, including Pierce's disease, phony peach disease, citrus variegated chlorosis, olive quick decline syndrome, almond leaf scorch and various other leaf scorch diseases. The pathogen is endemic in the Americas and is present in Iran. In the EU, it is reported in southern Apulia in Italy, on the island of Corsica and in the Provence‐Alpes‐Côte d'Azur region in France, as well as in the Autonomous r…

0106 biological sciences0301 basic medicine[SDV]Life Sciences [q-bio]Plant Science01 natural scienceslaw.inventionplant pestlawolive quick decline syndromePierce's diseaseolive quick.media_common2. Zero hungerpest riskquarantinefood and beverages3. Good healthHorticulturecitrus variegated chlorosis; European Union; leaf scorch; olive quick decline syndrome; pest risk; Pierce's disease; plant health; plant pest; quarantineSettore AGR/12 - PATOLOGIA VEGETALEWoody plantSciences exactes et naturellesPierce’s diseaseVeterinary (miscellaneous)Leaf scorchBiologyleaf scorchMicrobiology03 medical and health sciencesQuarantineOrnamental plantmedicinemedia_common.cataloged_instanceEuropean UnionEuropean unionEuropean Union pest risk plant health plant pest quarantine leaf scorch citrus variegated chlorosis Pierce’s disease olive quick decline syndromeHost (biology)citrus variegated chlorosisfungi15. Life on landbiology.organism_classificationmedicine.disease[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyScientific Opinion030104 developmental biologyAnimal Health and Welfare[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieAnimal Science and ZoologyParasitologyPEST analysisXylella fastidiosaplant health010606 plant biology & botanyFood Science
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Mangleticornia (Amaranthaceae: Salicornioideae) — a new sister for Salicornia from the Pacific coast of South America

2017

Mangleticornia ecuadorensis is a newly recognized monotypic genus from SW Ecuador and adjacent N Peru, where it had previously been identified as Salicornia fruticosa (Arthrocnemum fruticosum) or S. peruviana. It occurs on the coast adjacent to or in Equatorial-Pacific mangroves. Molecular phylogenetic evidence determines that this genus is distinct from and sister to Salicornia sensu lato, and is supported by morphological evidence. The genus is distinguished by a unique combination of characters that are otherwise rare in Salicornioideae: flowers without a visible perianth, anthers and stigmas exserted through pores in the fleshy cortical tissue of the segments, fruit included in the segm…

0106 biological sciences0301 basic medicinebiologySalicorniaWilldenowiaPlant ScienceAmaranthaceaebiology.organism_classification010603 evolutionary biology01 natural sciences03 medical and health sciences030104 developmental biologySensuInfructescenceBotanySalicornioideaeTaxonomy (biology)PerianthEcology Evolution Behavior and SystematicsWilldenowia
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Nacre evolution: a proteomic approach.

2009

AbstractFrom an evolutionary viewpoint, the molluscan nacre constitutes a fascinating object. This microstructure appeared early, in the Lower Cambrian period, about 530 million years ago, and since then, has been kept unchanged until today. Nacre is restricted to the conchiferan mollusks, where it occurs in t least three main classes, bivalves, gastropods and cephalopods. The aim of the present study is to investigate whether all nacres are built from the same “macromolecular tools”, proteins of the nacre matrix. To this end, we studied three new nacre models, the freshwater bivalve Unio pictorum, the cephalopod Nautilus macromphalus, and the gastropod Haliotis asinina, to which we applied…

0106 biological sciences0303 health sciencesFreshwater bivalveMaterials sciencebiologyHaliotis asininaUnio pictorumMacroevolutionbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials010603 evolutionary biology01 natural sciencesCephalopod[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials03 medical and health sciencesEvolutionary biologyBiophysics14. Life underwaterNautilus macromphalus[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsComputingMilieux_MISCELLANEOUS030304 developmental biology
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Assessment of genetically modified maize 4114 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐NL‐2014‐123)

2018

Abstract Maize 4114 was developed through Agrobacterium tumefaciens‐mediated transformation to provide protection against certain lepidopteran and coleopteran pests by expression of the Cry1F, Cry34Ab1 and Cry35Ab1 proteins derived from Bacillus thuringiensis, and tolerance to the herbicidal active ingredient glufosinate‐ammonium by expression of the PAT protein derived from Streptomyces viridochromogenes. The molecular characterisation data did not identify issues requiring assessment for food/feed safety. None of the compositional, agronomic and phenotypic differences identified between maize 4114 and the non‐genetically modified (GM) comparator(s) required further assessment. There were …

0106 biological sciences4114herbicide toleranceAgrobacteriumCry1F[SDV]Life Sciences [q-bio]Veterinary (miscellaneous)Cry34Ab1Context (language use)4114; Cry1F; Cry34Ab1; Cry35Ab1; GMO; herbicide tolerance; insect-resistant; maize (Zea mays); PAT; Regulation (EC) No 1829/2003TP1-1185Plant Science010501 environmental sciences01 natural sciencesMicrobiologyBacillus thuringiensisinsect‐resistantinsect-resistantTX341-641maize (Zea mays)0105 earth and related environmental sciences2. Zero hungerGenetically modified maizeAnimal healthbiologyNutrition. Foods and food supplyGMObusiness.industryChemical technologyCry35Ab1Regulation (EC) No 1829/2003maize (Zea mays)biology.organism_classificationGenetically modified organismBiotechnologyTransformation (genetics)Scientific Opinion13. Climate actionAnimal Science and ZoologyParasitologybusinessCry 1FPATRegulation (EC) No 1829/2003010606 plant biology & botanyFood SciencePotential toxicity
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An integrated analysis of micro- and macro-habitat features as a tool to detect weather-driven constraints: A case study with cavity nesters.

2017

The effects of climate change on animal populations may be shaped by habitat characteristics at both micro- and macro-habitat level, however, empirical studies integrating these two scales of observation are lacking. As analyses of the effects of climate change commonly rely on data from a much larger scale than the microhabitat level organisms are affected at, this mismatch risks hampering progress in developing understanding of the details of the ecological and evolutionary responses of organisms and, ultimately, effective actions to preserve their populations. Cavity nesters, often with a conservation status of concern, are an ideal model because the cavity is a microenvironment potentia…

0106 biological sciencesAtmospheric ScienceResearch FacilitiesPhysiologyOvipositionlcsh:MedicineKestrelMedicine (all); Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)01 natural sciencesGlobal Warming010605 ornithologyNesting BehaviorGeographical LocationsNestReproductive PhysiologyMedicine and Health SciencesNatural Selectionlcsh:ScienceAbiotic componentClimatologyeducation.field_of_studyMultidisciplinaryBiotic componentbiologyAnimal BehaviorEcologyMedicine (all)Falco naumanniEuropeHabitatItalyVertebratesClutchesResearch ArticleEvolutionary ProcessesClimate ChangePopulationAnimal Sexual BehaviorResearch and Analysis MethodsWeather Stations010603 evolutionary biologyBirdsAnimalseducationWeatherEcosystemFalconiformesAnalysis of VarianceBehaviorEvolutionary BiologyBiochemistry Genetics and Molecular Biology (all)Reproductive successlcsh:REndangered SpeciesOrganismsBiology and Life SciencesModels Theoreticalbiology.organism_classificationReproductive SuccessAgricultural and Biological Sciences (all)AmniotesPeople and PlacesLinear ModelsEarth Scienceslcsh:QZoologyPloS one
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Long-term changes in winter abundance of the barbastelle Barbastella barbastellus in Poland and the climate change - Are current monitoring schemes s…

2020

Warmer winters may lead to changes in the hibernation behaviour of bats, such as the barbastelle Barbastella barbastellus, which prefers to hibernate at low temperatures. The species is also known for its large annual fluctuations in the number of wintering individuals, so inference about population trends should be based on long-term data. Prior to 2005, analyses indicated stable or even increasing barbastelle population in Poland. We analysed the results of 13 winter bat counts (2005–2017) of the species from 15 of the largest hibernacula, and additional site of 47 small bunkers, in Poland. The total number of wintering individuals remained stable during the study period, because the barb…

0106 biological sciencesAtmospheric ScienceTime Factors010504 meteorology & atmospheric sciencesPhysiologySocial Sciences01 natural sciencesGeographical locationsAbundance (ecology)ChiropteraHibernationBatsMedicine and Health SciencesPsychologyClimatologyMammalseducation.field_of_studyMultidisciplinarybiologyGeographyAnimal BehaviorEcologyQRTemperatureEukaryotaCurrent (stream)EuropeBarbastella barbastellusGeographyResearch DesignVertebratesMedicineRegression AnalysisSeasonsNegative correlationEnvironmental MonitoringResearch ArticleCensusScienceClimate ChangePopulationClimate changeResearch and Analysis Methods010603 evolutionary biologyAnimalsEuropean Unioneducation0105 earth and related environmental sciencesBehaviorSurvey ResearchWinterOrganismsBiology and Life Sciencesbiology.organism_classificationAmniotesEarth SciencesPolandPeople and placesPhysiological ProcessesZoologyPloS one
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Oxidation, efflux, and isotopic fractionation of methane during autumnal turnover in a polyhumic, boreal lake

2007

[1] We studied the oxidation and efflux of methane (CH4) in a small, polyhumic lake, Mekkojarvi (southern Finland), during 6 weeks in autumn when the stability of the water mass first weakened, temporarily restabilized, and finally mixed completely. During the summer stratification period, CH4 had accumulated in the anoxic hypolimnion to high concentrations (>150 mmol m−3). Gradual mixing of the water column during the autumn allowed access to both oxygen and CH4 by aerobic methane-oxidizing bacteria (MOB) deeper in the water column. Thus the bulk (∼83–88%) of the CH4 accumulated in the hypolimnion was subsequently consumed by MOB while only 12–17% was lost from the lake to the atmosphere a…

0106 biological sciencesAtmospheric ScienceWater mass010504 meteorology & atmospheric sciencesSoil ScienceAquatic ScienceOceanography01 natural sciencesMethanechemistry.chemical_compoundWater columnIsotope fractionationGeochemistry and PetrologyEarth and Planetary Sciences (miscellaneous)0105 earth and related environmental sciencesEarth-Surface ProcessesWater Science and TechnologyHydrologyEcologyChemistry010604 marine biology & hydrobiologyPaleontologyForestryAnoxic watersGeophysics13. Climate actionSpace and Planetary ScienceIsotopes of carbonEnvironmental chemistryAnaerobic oxidation of methaneHypolimnionJournal of Geophysical Research
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