Search results for "nitrate reductase"
showing 10 items of 49 documents
Nitrate/oxygen co-sensing by an NreA/NreB sensor complex ofStaphylococcus carnosus
2013
In Staphylococci maximal induction of nitrate reductase (narGHJI genes) requires anaerobic conditions, the presence of nitrate, and the NreABC regulatory system. Aerobic regulation is effected by the NreB/NreC two-component system. The role of the nitrate receptor NreA in nitrate induction and its relation to aerobic regulation was analysed in Staphylococcus carnosus. Nitrate induction of a narG-lip reporter gene required presence of NreB/NreC. When nreA was deleted, nitrate was no longer required for maximal induction, suggesting that NreA is a nitrate regulated inhibitor of NreB/NreC. In vitro, NreA and mutant NreA(Y95A) decreased NreB phosphorylation in part or completely, which was due …
Involvement of nitrate reductase and pyoverdine in competitiveness of Pseudomonas fluorescens strain C7R12 in soil
2001
ABSTRACT Involvement of nitrate reductase and pyoverdine in the competitiveness of the biocontrol strain Pseudomonas fluorescens C7R12 was determined, under gnotobiotic conditions, in two soil compartments (bulk and rhizosphere soil), with the soil being kept at two different values of matric potential (−1 and −10 kPa). Three mutants affected in the synthesis of either the nitrate reductase (Nar − ), the pyoverdine (Pvd − ), or both (Nar − Pvd − ) were used. The Nar − and Nar − Pvd − mutants were obtained by site-directed mutagenesis of the wild-type strain and of the Pvd − mutant, respectively. The selective advantage given by nitrate reductase and pyoverdine to the wild-type strain was as…
Foliar characteristics, cambial activity and wood formation in Azadirachta indica A. Juss. as affected by coal–smoke pollution
2010
Abstract This study, aimed at elucidating changes in the foliar and cambial behavior in Azadirachta indica (Neem tree) due to coal-smoke pollution, has revealed inhibitory effects of pollution stress on leaf pigments concentrations, nitrate reductase activity and the contents of reducing sugars and total N content, whereas stimulatory effects were given on stomatal index and nitrate and sulphur contents. Under smoke effects, stomatal conductance was low, leading to a drop in the net photosynthetic rate and a rise in the internal CO2 concentration of leaf. Cambial reactivation in the stem was delayed at the polluted site. Although the total span of the cambial activity was reduced, greater a…
Characterization and role of nitric oxide production in Arabidopsis thaliana defense responses induced by oligogalacturonides
2011
Nitric oxide (NO) regulates a wide range of plant processes from development toenvironmental adaptation. In this study, NO production and its effects were investigated in aplant-pathogen context. The production of NO following Arabidopsis treatment witholigogalacturonides (OGs), an endogenous elicitor of plant defense, was assessed using the NOsensitive probe 4, 5-diamino fluorescein diacetate. Pharmacological and genetic approaches wereused to analyze NO enzymatic sources and its role in the Arabidopsis thaliana /Botrytis cinereainteraction. We showed that NO production involves both a L-arginine- and a nitrate reductase(NR)-pathways. OGs-induced NO production was Ca2+-dependent and modula…
Relative abundance of the membrane bound and periplasmic nitrate reductase in the environment
2007
Traking nitrate reducers and denitrifiers in the environment
2005
The ability to respire nitrate when oxygen is limited has been described in taxonomically diverse microorganisms including members of the alpha-, beta-, gamma- and epsilon-proteobacteria, high and low GC Gram-positive bacteria and even Archaea. Respiratory nitrate reduction is the first step of the denitrification pathway, which is important since it is the main biological process responsible for the return of fixed nitrogen to the atmosphere, thus completing the nitrogen cycle. During the last decade, considerable knowledge has been accumulated on the biochemistry and genetics of the nitrate reductases. In this paper, we summarize the recent progress in molecular approaches for studying th…
Biostimulant Potential of Humic Acids Extracted From an Amendment Obtained via Combination of Olive Mill Wastewaters (OMW) and a Pre-treated Organic …
2018
Olive mill wastewaters (OMW) detain contain significant levels of phenolic compounds with antimicrobial/phytotoxic activity and high amounts of undecomposed organic matter that may a high pollutant load that exerts negative effects on soil biology because of significant levels in phenolic compounds with antimicrobial/phytotoxic activity and limited biodegradability, and high amounts of undecomposed organic matter. Among OMW detoxification techniques, those focusing on oxidative degradation of phenolic compounds are relevant those focusing on oxidative degradation of phenolic compounds to reduce their toxicity. The composting (bio-oxidation) process in particular, exploits exothermic oxidati…
Modeling for the active site nitrate reductase. Oxidation of the complex [MovO(O2CC(S) CH3Ph)2]− by nitrate and nitrite in methanol
1998
Abstract Under acid conditions the [MoVIO2(O2CC(S)CH1Ph)2]2 reacts with thiols to yield the monomeric [MoVO(O2CC(S)CH3Ph)2] and disulfide. The reduced complex [MoVO(O2CC(S)CH3Ph)2]− can react with NO3− and NO2− in a one-electron step yeilding respectively NO2 and NO and the original molybdenum (VI)-dioxo complex. The experimental pseudo-first-order rate constant with respect to the Mo(V) complex at 25°C was found to be kobs=2.3×10−4s−1 for NO3− and kobs=1.0×10−2 for NO2−. Oxo transfers to and from the substrate have been coupled to produce a catalytic system which turns over the reaction RSH+(No3− or NO2−)+H+a 1 2 [ RS ] 2 +( NO ] 2 or NO )+ H 2 O , in which thiols, NO1− and NO2− serve as a…
Die Wirkung unterschiedlicher Lichtintensitäten auf die Nitratreduktase-Aktivität, den Gehalt an löslichen Proteinen und löslichen reduzierenden Zuck…
1977
Summary Plants of Sinapis alba (white mustard) grown under strong light conditions have a higher content of soluble reducing sugars, of soluble proteins and a higher dry wight during growth from germination to flowering. Furthermore, in these plants the nitrate reductase activity is essentially higher during the vegetative development, but before the beginning of the flowering phase the activity is less than the level of plants grown under low light intensities. The results obtained indicate a direct correlation between nitrate reductase activity and the content of soluble proteins. In the high-light plants both components show distinct maxima 13—14 days after sowing the seeds. The enhancem…
Effect of titanium (IV) application on some enzymatic activities in several developing stages of red pepper plants
1994
Abstract The effect of two titanium (IV) compounds, titanium ascorbate and titanium chloride, on some enzymatic activities, such as catalase, peroxidase, lipoxygenase and nitrate reductase in seeds, embryos, and seedlings and adult plants of red pepper (Capsicum annuum L.), was studied. A stimulatory effect of titanium was observed for every iron‐depending enzyme studied at all developing stages as well as for nitrate reductase but only for whole plants. This occurs because Ti+3/Ti+4 catalizes the activity of iron and enhances iron‐dependent enzymes.