Search results for "nonhuman"

showing 7 items of 47 documents

The deubiquitinase USP11 is a versatile and conserved regulator of autophagy

2021

Autophagy is a major cellular quality control system responsible for the degradation of proteins and organelles in response to stress and damage to maintain homeostasis. Ubiquitination of autophagy-related proteins or regulatory components is important for the precise control of autophagy pathways. Here, we show that the deubiquitinase ubiquitin-specific protease 11 (USP11) restricts autophagy and that KO of USP11 in mammalian cells results in elevated autophagic flux. We also demonstrate that depletion of the USP11 homolog H34C03.2 in Caenorhabditis elegans triggers hyperactivation of autophagy and protects the animals against human amyloid-β peptide 42 aggregation-induced paralysis. USP11…

autophagyhAβ42 human amyloid-β protein 1 to 42Lipid kinase activityPI(3)P phosphatidylinositol-3-phosphatemTORC1BiochemistryCell LineGene Knockout Techniqueschemistry.chemical_compoundubiquitinAnimalsHumansULK1 unc-51-like autophagy activating kinase 1WIPI WD-repeat domain phosphoinositide-interacting proteinPI3KC3-C1Caenorhabditis elegansCaenorhabditis elegans ProteinsmTORC1Molecular BiologyMechanistic target of rapamycinUSP11 ubiquitin-specific protease 11proteostasisAmyloid beta-PeptidesS6K S6 kinasebiologyPhosphatidylinositol 3-phosphateAutophagyDUB deubiquitinaseLFQ label-free quantificationIP immunoprecipitationNHT nonhuman targetingPI3KC3-C1 class III phosphatidylinositol 3-kinase complex ICell BiologyACN acetonitrile amyloid-βNRBF2 nuclear receptor-binding factor 2Peptide FragmentsCell biologydeubiquitinase (DUB)ProteostasischemistryProteotoxicitymTORC1 mechanistic target of rapamycin complex 1biology.proteinAutophagy-Related Protein-1 HomologBSA bovine serum albuminThiolester HydrolasesResearch ArticleJournal of Biological Chemistry
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The influence of backslopping on lactic acid bacteria diversity in tarhana fermentation

2020

Tarhana is produced at batch systems in which the microbiota has changed accordingly to the microbial load from ingredients. In order to stabilize the microbiota, the effects of backslopping carried out under different temperature regimes (25 and 30 °C), pH (3.70 and 4.00) and inoculation rates (5, 10 and 15%) on lactic acid bacteria (LAB) diversity were determined in tarhana dough. LAB and Total Aerobic Mesophilic Bacteria (TAMB) numbers increased in all tarhana dough samples subjected to backslopping. Temperature and pH significantly affected the microbiological diversity of tarhana whereas the different inoculation rates did not. Tarhana dough showed complex tarhana microbiota following …

genomic DNAtomatochemistry.chemical_compoundCereal fermentationpepperLactobacillalesLactococcusFermented Foods and BeveragesLactic acid bacteriageneticsFood scienceyoghurtfermentationonionbiodiversity0303 health sciencesbiologyLactobacillus brevisBacksloppingpHMicrobiotaTemperaturefermented productGeneral MedicineBreadHydrogen-Ion ConcentrationLactobacillus brevisLactic acidStarter cultureclassificationBatch Cell Culture TechniquesTarhana microbiotasodium chlorideFermented Foodsmicrobial communityMesophilelactic acid bacteriumRNA 16Sgene sequenceArticlewheat flour03 medical and health sciencesinoculationproceduresacidity030304 developmental biologydoughnonhuman030306 microbiologyisolation and purificationmicrobiologyStreptococcusbiology.organism_classificationLactobacilluschemistrymicrobial diversityWeissellaCarnobacteriumFermentationpolymerase chain reaction denaturing gradient gel electrophoresismicrofloraLactobacillus alimentariusbatch cell culturemetabolismLactobacillus alimentariusLactobacillus plantarumBacteriaEnterococcusLeuconostocSettore AGR/16 - Microbiologia AgrariaFood ScienceLactobacillus plantarum
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Intra-Acting Food Citizenship in Community-Supported Agriculture in Finland

2023

Citizens are called upon to become active participants in creating a more sustainable food system. As food citizens, people participate in defining and constructing their food systems according to their needs and values. In food policies, the concept of food citizenship is often left undefined or with reference only to individual activities. In the food citizenship literature, the role of nonhuman agency in constituting food citizenship needs more examination. Here we investigate food citizenship activities in a citizen-led community-supported agriculture group and explore the role of materiality in constituting food citizenship. We ask (1) what is the role of material-discursive arrangemen…

intra-actionnonhuman agencycitizenshipfood citizenshipruokajärjestelmätosallistaminencommunity-supported agriculturekansalaisuuskansalaistoimintaosallistuminen
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Temperature effects explain continental scale distribution of cyanobacterial toxins

2018

Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect…

light climate0106 biological sciencesthermoclineBacterial toxinstoksiinitlimit of quantitationToxines bacterianesMicrocystin-LRToxicology01 natural sciencesAnatoxin-aanalogs and derivativesBLOOMSDirect EffectsuracilWater Pollutantschemistry.chemical_classificationTemperaturesFRESH-WATERlatitudemaximum buoyancy frequency6. Clean waterclimate changeIndirect effectsEUTROPHICATIONmicrocystin RRarticlesGROWTHlämpötilaLAKESmicrocystin; anatoxin; cylindrospermopsin; temperature; direct effects; indirect effects; spatial distribution; European Multi Lake Surveyepilimnetic temperatureta1172cyanobacteria lakes climate warming microcystin; anatoxin; cylindrospermopsin; temperature; direct effects; indirect effects; spatial distribution; European Multi Lake SurveyZoologyArticlewater pollutantMICROCYSTIS-AERUGINOSAAlkaloidsSettore BIO/07 - ECOLOGIANATURAL SCIENCES. Biology.Spatial distributionMicrocystis aeruginosaUracillakesyanobakteeritIndirect Effectsliquid chromatography-mass spectrometry1172 Environmental sciencesEkologinutrient010604 marine biology & hydrobiologylcsh:RmicrobiologyClimatic changesmicrocystin LRAnatoxinLakesSpatial Distributionchemistrynodularinmicrobial diversityphytoplanktonta1181CylindrospermopsinTropanesCyanobacteriaAquatic Ecology and Water Quality ManagementanalysisHealth Toxicology and Mutagenesislcsh:Medicineenvironmental parameters010501 environmental sciencesmedicine.disease_causenitrogenchemistry.chemical_compoundsea surface temperatureenvironmental factorddc:550Canvi climàticphosphorusPRIRODNE ZNANOSTI. Biologija.limit of detectionEcologyCyanobacteria ToxinsbiologyTemperaturelevinneisyysmicrocystin ; anatoxin ; cylindrospermopsin ; temperature ; direct effects ; indirect effects ; spatial distribution ; European Multi Lake SurveyNodularintropane derivativeEuropeDAPHNIA-MAGNAİndirect EffectsCylindrospermopsinDirect effectsmicrobial communityEnvironmental Monitoringhigh performance liquid chromatographyMicrocystinsClimate ChangeBacterial ToxinsMicrocystinMicrocystinCyanobacteriavälittömät oikeusvaikutuksetcyanobacteriumddc:570geographic distributionmedicinebacterial toxincontrolled studyddc:610Institut für Biochemie und Biologie0105 earth and related environmental sciencesnonhumanWIMEKToxinlongitudePHYTOPLANKTON ASSEMBLAGESEuropean Multi Lake SurveyAquatic EcologyNITROGEN AVAILABILITYanatoxin aAquatische Ecologie en Waterkwaliteitsbeheerbiology.organism_classificationClimatic changeCLIMATE13. Climate actionresponse variableCanvis climàtics
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Problem Talk in Management Group Meetings

2019

This naturalistic study focuses on problem talk (PT) in hospital management group meetings. The study aims to understand how PT constitutes the hospital organization through the different uses of PT within the meetings, and, therefore, to understand the organizing role of these meetings. The communication as constitutive of organization (CCO) perspective forms the theoretical background of the research. The results of the qualitative analysis show that PT comprises many intertwined tasks that aim to perform the meetings, enhance problem solving, and maintain the relational level of group life. Thus, PT is much more than merely solving problems. In PT, problems are discussed from the viewpo…

nonhuman agencyMedical educationvuorovaikutusSocial PsychologykokouksetGroup (mathematics)05 social sciencesorganisaatiot050801 communication & media studies0508 media and communicationsNaturalistic observationcommunication as constitutive of organizationproblem solvinggroup communicationmeetings0502 economics and businessCommunication in small groupsongelmanratkaisuPsychology050203 business & managementApplied Psychologyviestintä
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Posthuman(ist) Feminism, Feminist Posthumanities

2018

posthumantasa-arvononhumanfeminismiei-ihmisetposthumanismiantroposeeniihmisetequalityetiikka
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Double copies of blaKPC-3::Tn4401a on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy

2015

ABSTRACT A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla KPC genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla KPC-3 gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla KPC-3 ::Tn 4401a caused by intramolecular transposition events were detected in the plasmid.

transposonsequence analysispolymerase chain reactionDrug ResistanceGene DosageSettore MED/42 - Igiene Generale E Applicatabacterial proteinbeta-Lactamaseopen reading framecarbapenemasePlasmidminocyclineplasmid DNAmeropenemPharmacology (medical)geneticscolistincefpodoximeceftazidime610 Medicine & healthCarbapenemBacterialpolymyxin Btimentingene expression regulationbacteriumKlebsiella pneumoniae carbapenemase 3 producing Klebsiella pneumoniae3. Good healthantiinfective agentmicrobial sensitivity testKlebsiella pneumoniaeItalypriority journaltigecyclineMultipleclone (Java method)cefotaxime030106 microbiologyKlebsiella pneumoniae carbapenemase 3tobramycinMicrobial Sensitivity Testsgentamicinpiperacillin plus tazobactamchemistryGene dosageArticleMicrobiology03 medical and health sciencesComplete sequenceClone CellOpen Reading FramesertapenemBacterial Proteinsmultidrug resistanceextensively drug resistant bacteriumAnti-Bacterial AgentcefepimePharmacologylevofloxacinmicrobiologycefoxitinbiochemical phenomena metabolism and nutritionbacterial infections and mycosesVirologyAnti-Bacterial Agents; Bacterial Proteins; Carbapenems; Clone Cells; Drug Resistance Multiple Bacterial; Gene Dosage; Italy; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Open Reading Frames; Plasmids; beta-Lactamases; DNA Transposable Elements; Gene Expression Regulation Bacterial; Pharmacology (medical); Pharmacology; Infectious Diseasesantibiotic sensitivityClone CellsKlebsiella InfectionsceftriaxoneCarbapenemsbacterial genetics0301 basic medicinemolecular cloningSettore MED/07 - Microbiologia E Microbiologia ClinicaKlebsiella pneumoniaeTransposition (music)Drug Resistance Multiple Bacterialpolycyclic compoundsgenetic screeningcell clonecarbapenem derivativeKlebsiella infectionunclassified drugAnti-Bacterial AgentsInfectious Diseasesbacterial genePlasmidsenzymologydoripenemBiologyminimum inhibitory concentrationbeta-Lactamasesbeta lactamaseMechanisms of ResistanceciprofloxacinAmikacin; aztreonam; carbapenemase; cefepime; cefotaxime; cefoxitin; cefpodoxime; ceftazidime; ceftriaxone; ciprofloxacin; colistin; cotrimoxazole; doripenem; doxycycline; ertapenem; gentamicin; imipenem; Klebsiella pneumoniae carbapenemase 3; levofloxacin; meropenem; minocycline; piperacillin plus tazobactam; plasmid DNA; polymyxin B; tigecycline; timentin; tobramycin; unclassified drug; antiinfective agent; bacterial protein; beta lactamase; carbapenem derivative; transposon antibiotic sensitivity; Article; bacterial gene; bacterial genetics; bacterial strain; bacterium; bacterium detection; bacterium isolation; Escherichia coli; extensively drug resistant bacterium; gene dosage; genetic screening; Italy; Klebsiella pneumoniae; Klebsiella pneumoniae carbapenemase 3 producing Klebsiella pneumoniae; minimum inhibitory concentration; molecular cloning; nonhuman; polymerase chain reaction; priority journal; sequence analysis; cell clone; chemistry; drug effects; enzymology; gene expression regulation; genetics; isolation and purification; Klebsiella infection; Klebsiella pneumoniae; metabolism; microbial sensitivity test; microbiology; multidrug resistance; open reading frame; plasmid; transposon Anti-Bacterial Agents; Bacterial Proteins; beta-Lactamases; Carbapenems; Clone Cells; DNA Transposable Elements; Drug Resistance Multiple Bacterial; Gene Dosage; Gene Expression Regulation Bacterial; Italy; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Open Reading Frames; Plasmidsplasmidbacterium isolationEscherichia coliGeneAmikacinbacterium detectionnonhumandoxycyclineisolation and purificationGene Expression Regulation Bacterialbiology.organism_classificationbacterial straincotrimoxazoleOpen reading frameDNA Transposable Elementdrug effectsDNA Transposable Elementsmetabolismaztreonamimipenem
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