Search results for "oligo"

Identification of HLA-A*0201-Restricted T Cell Epitopes Derived from the Novel Overexpressed Tumor Antigen Calcium-Activated Chloride Channel 2

2002

Abstract Vaccination against tumor Ags may become a promising treatment modality especially in cancer types where other therapeutic approaches fail. However, diversity of tumors requires that a multitude of Ags become available. Differential expression in normal vs cancerous tissues, both at the mRNA and the protein level, may identify Ag candidates. We have previously compared transcripts from squamous cell lung cancer and normal lung tissue using differential display analysis, and found a transcript that was overexpressed in malignant cells and was identical with the calcium-activated chloride channel 2 (CLCA2) gene. We have now selected HLA-A2-restricted peptides from CLCA2, and have gen…

Enhanced dendritic cell maturation by TNF-alpha or cytidine-phosphate-guanosine DNA drives T cell activation in vitro and therapeutic anti-tumor immu…

2000

Abstract Dendritic cells (DC) manipulated ex vivo can induce tumor immunity in experimental murine tumor models. To improve DC-based tumor vaccination, we studied whether DC maturation affects the T cell-activating potential in vitro and the induction of tumor immunity in vivo. Maturation of murine bone marrow-derived DC was induced by GM-CSF plus IL-4 alone or by further addition of TNF-α or a cytidine-phosphate-guanosine (CpG)-containing oligonucleotide (ODN-1826), which mimics the immunostimulatory effect of bacterial DNA. Flow cytometric analysis of costimulatory molecules and MHC class II showed that DC maturation was stimulated most by ODN-1826, whereas TNF-α had an intermediate effec…

In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein ofMycobacterium tuberculosis

2004

The 16-kDa protein of Mycobacterium tuberculosis provokes specific immune responses; it is thus a target for the development of peptide-based diagnostic reagents and subunit vaccines. Previous studies have demonstrated the presence of several regions containing murine and human T-cell epitopes. Within the 91–110 immunodominant domain, we found that peptides comprising the sequence of 91SEFAYGSFVRTVSL104 elicit specific T-cell responses in both human T-cell clones and human peripheral blood mononuclear cells (PBMC) from PPD+ (purified protein derivative) individuals. Elongation of this peptide towards the C-terminal end did not provide more effective peptides, but the removal of residue 91Se…

Abinieku sugu daudzveidība un nārsta ūdenstilpes Aizsargājamo ainavu apvidū „Ādaži”

2015

Darba ietvaros veikti 5 abinieku sugu (Rana arvalis; Rana lessonae; Epidalea calamita; Pelobates fuscus; Triturus vulgaris) iespējamo nārstošanas vietu apsekojumi Ādažu poligonā, kur notiek militārās apmācības. Darba mērķis: izpētīt ūdenstilpju ekoloģiskās īpatnības un analizēt, kādi faktori veido sugu nārsta nišas. Lauka darbi veikti Ādažu poligona C sektorā, kā parauglaukumus ieviešot apsekotās ūdenstilpes. Apkopota informācija par ūdenstilpju koordinātēm, lielumu, dziļumu, veģetāciju un tajā sastopamajiem abiniekiem. Galvenie rezultāti: izdalās faktori, kas liecina par katras sugas specifiskumu: T. vulgaris - ūdenstilpju dziļums; P.fuscus - makrofītu aizaugums (%); R.lessonae – grīšļu un…

The Saccharomyces cerevisiae flavodoxin-like proteins Ycp4 and Rfs1 play a role in stress response and in the regulation of genes related to metaboli…

2011

SPI1 is a gene whose expression responds to many environmental stimuli, including entry into stationary phase. We have performed a screening to identify genes that activate SPI1 promoter when overexpressed. The phosphatidylinositol- 4-phosphate 5-kinase gene MSS4 was identified as a positive activator of SPI1. Another SPI1 transcriptional regulator isolated was the flavodoxin-like gene YCP4. YCP4 and its homolog RFS1 regulate the expression of many genes during the late stages of growth. The double deletion mutant in YCP4 and its homolog RFS1 has an impact on gene expression related to metabolism by increasing the expression of genes involved in hexose transport and glycolysis, and decreasi…

EMODINAMICA DEL TALAMO

2009

Small Molecules Targeting and Modulating Toxic Tau Oligomeric Strains

Alzheimer’s disease (AD) is one of over 18 different disorders known as tauopathies, characterized by the pathological aggregation and accumulation of tau, a microtubule-associated protein. Tau aggregates are heterogeneous and can be divided into two major groups: large metastable neurofibrillary tangles (NFTs) and oligomers. Recently, it has been shown that tau oligomers are highly toxic in vitro and efficient seeds for the propagation of pathology as compared to NFTs. While the toxicity of recombinant tau oligomers has been studied extensively, within the same aggregation state, tau exhibits conformational differences, termed tau oligomeric strains. Due to the dynamic nature of these stra…

Hypoplastic amelogenesis imperfecta with multiple impacted teeth - report of two cases

2010

Amelogenesis Imperfecta (AI) represents a group of developmental conditions, genomic in origin, which affect the structure and clinical appearance of enamel of all or nearly all the teeth in a more or less equal manner. It is usually inherited either as an X-linked, autosomal dominant or autosomal recessive trait. The enamel may be hypoplastic, hypomineralised or both and affected teeth may be discolored, sensitive or prone to disintegration. Diagnosis is based on the family history, pedigree plotting and meticulous clinical observation. The treatment of patients with AI should start with early diagnosis and intervention to prevent latter restorative problems. Herein, we present two case re…

Strategic interactions on differentiated markets and public policies

2017

In economy, the competition analysis among firms have a significance importance because of the complexity of some elements. This thesis examines the strategic interactions on differentiated markets in general equilibrium while proposing the public policies to reduce the distortions due to the imperfect behaviors. This thesis takes into account the differentiation of products in order to obtain the new conclusions and/or to extend those existing. We thus pursues two objectives. Fisrt, the construction of a model of imperfect competition on differentiated markets in a general equilibrium framework. Second, the evaluation of the consequences of the model in terms of economic policies. At begin…

Synthesis of molecularly uniform model oligomers for 1,5-naphthalene diisocyanate and 1,4-butanediol based polyurethanes by using the 3,5-di-tert-but…

1997

A modified benzyloxycarbonyl (BOC) protective group, the 3,5-di-tert-butyl substituted BOC (3,5-tBBOC) group, was introduced to the stepwise synthesis of molecularly uniform oligourethanes based on 1,5-naphthalene diisocyanate (NDI) and 1,4-butanediol (BDO) for the protection of the amino functions of the starting material 1,5-naphthalene-diamine (NDA). The tert-butyl substituents of the 3,5-tBBOC group significantly improved the solubility of the intermediate products in the oligourethane synthesis. Thus, applying this modified protective group, reactions could be carried out, which were not possible with the conventional BOC group, due to the insolubility of the compounds in all suitable …