Search results for "oligo"
showing 10 items of 1298 documents
Spent Coffee Grounds Extract, Rich in Mannooligosaccharides, Promotes a Healthier Gut Microbial Community in a Dose-Dependent Manner.
2019
Coffee is one of the most consumed beverages around the world, and as a consequence, spent coffee grounds are a massively produced residue that is causing environmental problems. Reusing them is a major focus of interest presently. We extracted mannooligosaccharides (MOS) from spent coffee grounds and submitted them to an in vitro fermentation with human feces. Results obtained suggest that MOS are able to exert a prebiotic effect on gut microbiota by stimulating the growth of some beneficial genera, such as Barnesiella, Odoribacter, Coprococcus, Butyricicoccus, Intestinimonas, Pseudoflavonifractor, and Veillonella. Moreover, short-chain fatty acids (SCFA) production also increased in a dos…
Global-Scale Environmental Niche and Habitat of Blue Shark (Prionace glauca) by Size and Sex: A Pivotal Step to Improving Stock Management
2022
25 pages, 10 figures, 2 tables, supplementary material https://www.frontiersin.org/articles/10.3389/fmars.2022.828412/full#supplementary-material.-- Data Availability Statement: The blue shark habitat data are available on the JRC Data Catalogue (https://data.jrc.ec.europa.eu/dataset?q=fish%20habitat) (or upon request) and habitat maps are available online (https://fishreg.jrc.ec.europa.eu/web/fish-habitat/habitatmapping). Chlorophyll-a data are available online (https://oceancolor.gsfc.nasa.gov/cgi/l3). The abiotic environmental and mesopelagic micronekton data are available online (https://marine.copernicus.eu/access-data).
Efficient stabilisation of a dihydrogenphosphate tetramer and a dihydrogenpyrophosphate dimer by a cyclic pseudopeptide containing 1,4-disubstituted …
2017
A cyclic pseudooctapeptide 2 is described containing 1,4-disubstituted 1,2,3-triazole moieties. This compound features eight converging hydrogen bond donors along the ring, namely four amide NH and four triazole CH groups, which enable 2 to engage in interactions with anions. While fully deprotonated sulfate anions exhibit only moderate affinity for 2, protonated anions such as dihydrogenpyrophosphate and dihydrogenphosphate anions are strongly bound. Complexation of the phosphate-derived anions involves sandwiching of a dihydrogenpyrophosphate dimer or a dihydrogenphosphate tetramer between two pseudopeptide rings. X-ray crystallography provided structural information, while 1H NMR spectro…
Study of Thermotoga maritima ß-galactosidase: immobilization, engineering and phylogenetic analysis
2016
La actividad de las lactasas fue descrita por Beijerink en 1889. El origen de dicha actividad era la ß-galactosidasa de la levadura Kluyveromyces lactis capaz de hidrolizar la lactosa en sus dos componentes, galactosa y glucosa. En la actualidad las ß-galactosidasas tienen dos aplicaciones principales, por un lado se utilizan para la obtención de productos libres en lactosa y por otro lado para la síntesis de galactooligosacáridos (GOS), a algunos de los cuales se les han asociado propiedades prebióticas. Esta doble capacidad de la enzima se da gracias al mecanismo de retención que comparte con otras proteínas del clan GH-A en la clasificación del CAZy.En este trabajo hemos explorado la pos…
Sinbiotiska produkta iegūšana, veicot no piena permeāta iegūta galaktooligosaharīdu (GOS) maisījuma selektīvu fermentāciju ar probiotiskiem celmiem
2022
Piena permeāts un tajā esošā laktoze kā substrāts var tikt izmantots ogļhidrātu biokonversijas pētījumos, kā arī dažādu mikroorganismu kultivēšanā, lai veidotu produktus ar pievienoto vērtību. Darba mērķis bija izvērtēt iespēju iegūt galaktooligosaharīdu (GOS) un pienskābo baktēriju saturošu sinbiotisku produktu, veicot selektīvu fermentāciju maisījumā, kas iegūts fermentatīvās GOS biosintēzes laikā no piena permeāta. Veicot fermentatīvu sintēzi ar ß-galaktozidāzēm, tika iegūts un aprakstīts GOS saturošs maisījums, kurā vēlāk tika veikta selektīva fermentācija ar probiotiskajiem Lactobacillus spp. celmiem, izvērtējot šo celmu ogļhidrātu preferences un izdzīvotību. Veicot fermentatīvu sintēz…
Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model
2006
Abstract Background The TGFβ1-induced signal transduction processes involved in growth and differentiation are only partly known. The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFβ1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model epithelial cell differentiation seen in intestine. That model has not been used for large scale gene expression studies, such as microarray method. Therefore the gene expression changes were studied in undifferentiated and differentiated three-dimensional T84 cultures with cDNA microarray method in order to study the molecular changes and find new play…
Oligonucleotides-decorated-poly(N-vinyl pyrrolidone) nanogels for gene delivery
2013
Pulsed electron-beam irradiation of a semi-dilute poly(N-vinyl pyrrolidone) (PVP) aqueous solution in the presence of acrylic acid has led to a carboxyl functionalized nanogel system. Nanoparticles hydrodynamic size and surface charge density, in water and as a function of pH, were investigated by dynamic light scattering and laser doppler velocimetry, respectively. Nanogels (NGs) were proved not to be cytotoxic at the cellular level. Indeed, they rapidly bypass the cellular membrane to accumulate in specific cell portions of the cytoplasm, in the perinuclear area. The availability of pendant carboxyl groups on the crosslinked PVP NGs core prompted us to attempt their decoration with a sing…
Cholesterol-Streptolysin O Interaction: An EM Study of Wild-Type and Mutant Streptolysin O
1998
We present transmission electron microscopical data from negatively stained specimens of cholesterol following interaction with the thiol-activated bacterial toxin streptolysin O (SLO) (wild-type and a number of cysteine substitution mutants), with and without chemical modification of the cysteine residues. Two experimental systems were used, one with an aqueous suspension of cholesterol microcrystals and the other with immobilized thin planar cholesterol crystals attached to a carbon film. In both systems the wild-type SLO and two cytolytically active mutants, Cys 530 --Ala (C530A) and Ser 101 --Cys (S101C), readily generated the characteristic SLO arc- and ring-like oligomers on the surfa…
Optical and electrical properties of oriented thin films of oligomer containing betaine-type moiety in side chain
2005
Non-linear optical and electrical properties of polymer films obtained by dipole orientation of active units are reported. Novel polar oligomer with N-(indan-1,3-dion-2-yl)pyridinium betaine (IPB) as a side group is studied. Orientation of polar groups in oligomer thin films causes an increase of the photo-induced change of surface potential on irradiation in the region of photo-induced electron transfer (PIET) where the IPB group exhibits a reversible change of the value and sign of the dipole moment. At longer wavelengths, the value of the surface potential of the oligomer may be determined by transport of photogenerated charge carriers.
The grapevine LysM receptor-like kinase VvLYK5-1 mediates chitin-triggered immunity
2021
The establishment of defense reactions to protect plants against invading pathogens first requiresthe recognition of Microbe-Associated Molecular Patterns (MAMPs), detected by plasmamembrane-bound Pattern Recognition Receptors (PRRs). These MAMPs, also termed elicitors, areused in several biocontrol products that are gradually developing to reduce the use of chemicalpesticides in agriculture. Chitin, the main component of fungal cell walls, as well as its deacetylatedderivative, chitosan, are two chitooligosaccharides (COS) that can be found in some of theseproducts. Unfortunately, the mechanism allowing the perception of these molecules is still poorlyunderstood in Vitis vinifera, sometime…