Search results for "oligo"
showing 10 items of 1298 documents
Melanoma cells release extracellular vesicle which contain H1° linker histone as well as RNA-binding proteins which bind to the H1° mRNA
2015
We previously demonstrated that G26/24 oligodendroglioma cells release EVs that contain proteins, such as FasL and TRAIL, which induce apoptosis in rat cortical neurons [1] and astrocytes [2]. We also reported that cancer cells use EVs for transferring, into the environment [3], proteins such as extracellular matrix remodelling proteases [4], and H1°, a differentiation-specific histone [5]. In particular, by releasing H1°, cells could escape differentiation cues [5]. To verify the role of EVs in releasing specific proteins and mRNAs, in this study we used as a model A375 melanoma cells. METHODS EVs were purified from cell culture media as previously reported [1, 2]. T1 RNase-protection assa…
Cancer cells can affect behaviour of neighbouring cells by transferring molecules through extracellular vesicles
2017
Most cells release into the extracellular space membrane-bound structures of different sizes, origin and composition, collectively called extracellular vesicles (EVs) [1]. Tumor cells are much more active than normal cells in producing EVs. Because of this property, they are able to transfer both nucleic acids and proteins to the surrounding normal cells, thus inducing in these latter at least some transformed behavior. We previously showed that EVs produced by G26/24 oligodendroglioma cells can horizontally transfer to their neighbours radioactive proteins [2]. In addition, EVs released by these cells contain pro-apoptotic proteins, such as TRAIL and Fas-Ligand, able to induce apoptosis in…
Melanoma cells release extracellular vesicles which contain RNA-binding proteins able to bind the mRNA encoding histone H1°
2015
Extracellular vesicles (EVs) are produced by most prokaryotic and eukaryotic cells; tumour cells, however, release much higher amounts of EVs, which contain cancer-specific proteins and RNAs. Molecules carried by EVs are captured by surrounding cells, which then undergo profound phenotypic modifications. G26/24 oligodendroglioma cells release, for example, EVs containing FasL and TRAIL, which induce apoptosis in rat cortical neurons and astrocytes in culture. By metabolic labelling of cells, EV-mediated horizontal transfer of radioactive proteins was clearly demonstrated. Among the proteins present in EVs produced by oligodendroglioma cells, extracellular matrix remodelling proteases, and t…
RNA as a carrier of epigenetic information
2017
Both prokaryotic and eukaryotic cells release into the extracellular matrix membrane-bound structures of different sizes, origin and composition, collectively called extracellular vesicles (EVs) [1]. Tumor cells, in particular, use EVs to transfer both nucleic acids and proteins to the surrounding normal cells, thus inducing in them transformed behaviours or killing them. G26/24 oligodendroglioma cells, for example, transfer by EVs pro-apoptotic proteins, such as TRAIL and Fas-Ligand [2], extracellular matrix remodelling proteases (such as ADAMTS) [3], and even the H1.0 histone protein [4]. Another tumour cell line, with a different tissue origin (A375 melanoma cells) releases into the medi…
La tecnologia del DNA-microarray per l'identificazione di specie microbiche su superfici e nell'aerosol di ambienti confinati
2009
La tecnologia del DNA microarray è utilizzata nei nostri laboratori per lo sviluppo di piattaforme multi-diagnostiche finalizzate alla identificazione di microrganismi. Il sistema è applicabile a campioni biologici prelevati in ambienti confinati, dove funghi e batteri rappresentano una delle componenti principali. Questi microrganismi, sono in grado di svilupparsi, in stretta relazione con determinati parametri come: i valori di temperatura e umidità relativa, le caratteristiche chimico fisiche dei materiali, e delle superfici, la tipologia di aerosol di questi ambienti. L’instaurarsi di consorzi microbici complessi e stabili oltre ad accelerare il degrado superficiale dei materiali, può c…
La città di Alphabet. Architettura per prosumers.
2021
Attraverso alcuni aspetti salienti dell’ideazione, della produzione e dell’opposizione al progetto del Toronto Quayside di Sidewalk labs, l’articolo discute l’influenza delle infrastrutture digitali per l’informazione sul progetto della casa e del quartiere. L’ampliamento delle attività svolte da remoto nello spazio domestico, enfatizzato dal quotidiano della pandemia da Covid 19, costituisce per gli oligopolisti planetari degli urban data come Alphabet un utile volano pubblicitario e un acceleratore. La fusione dei nuovi vessilli della tecnoemancipazione e della sostenibilità in ipotesi sperimentali che mirano a costruirsi sia per intero che per parti realizza un nuovo orizzonte in cui lo …
THE FINGERPRINT OF THE HUMAN GASTROINTESTINAL TRACT MICROBIOTA: A HYPOTHESIS OF MOLECULAR MAPPING
2017
The precise etiology of Inflammatory Bowel Disease (IDB) remains unclear and several factors are believed to play a role in its development and progression, including the composition of microbial communities resident in the gastrointestinal tract. Human intestinal microbiota are extensive with at least 15,000-36,000 bacterial species. However, thanks to the new development in sequencing and molecular taxonomic methodologies, our understanding of the microbiota population composition, dynamics, and ecology has greatly increased. Intestinal microbiota play a critical role in the maintenance of the host intestinal barrier homeostasis, while dysbiosis, which involves reduction in the microbiome…
The Sylos Postulate reconsidered
2016
This chapter makes a textual comparison between the 1957 2nd Italian edition and the 1962 1st American edition of Oligopoly and Technical Progress, showing that Sylos Labini added to the later edition some fresh sections concerning new firms’ entry and incumbents’ reaction which partially contradict Modigliani’s 1958 presentation of the Sylos Postulate. The theoretical differences between Sylos Labini and Modigliani with regard to the assumption of constant output are traced back to their different modelling strategies on how to tackle the issue of external firms’ conjectures in determining the long-run oligopoly equilibrium price.
Chamberlin, Edward Hastings
2016
The entry describes the life and analytical contributions of the US economist Edward Chamberlin (1899 -1967). It reconstructs the development of Chamberlin's thought on the issue of monopolistic competition and compares it with the Economics of Imperfect Competition by Joan Robinson.
Paolo Sylos Labini Vindicated
2017
In the first part of our chapter we critically discuss i) Modigliani’s 1958 interpretation of Sylos Labini’s Oligopolio e Progresso Tecnico (1957), ii) the following debate concerning the Sylos Postulate −the assumption according to which “potential entrants behave as though they expected existing firms to adopt the policy most unfavourable to them, namely, the policy of maintaining output while reducing the price (or accepting reductions) to the extent required to enforce such an output policy” − and iii) the incumbent’s choice of productive capacity to install as strategic entry deterrence. In the second part of the chapter we develop a model in which, as in Dixit (1980), there are three …