Search results for "ops"

showing 10 items of 5435 documents

Quantitative DNA measurements in malignant and benign lesions of the upper aerodigestive tract.

1992

Thirty-six patients underwent biopsy of clinically suspicious lesions of the mucosa of the upper aerodigestive tract. The biopsy material was evaluated histologically and cytologic smears out of the same lesion underwent quantitative DNA analysis using a numerical index derived from the single cell DNA content. In 30 patients, histologically diagnosed squamous cell cancer was confirmed by DNA analysis with the malignancy grade correlating with the morphologic differentiation of the tumor. Six lesions were histologically benign: i.e. dysplasia (n = 3), hyperplasia (n = 2) and chronic inflammation (n = 1). DNA analysis confirmed the benign nature in 4 cases, but in 2 cases of dysplasia, a dia…

AdultMalePathologymedicine.medical_specialtyBiopsyMalignancyDigestive System NeoplasmsModels BiologicalLesionCytologyBiopsymedicineHumansAgedmedicine.diagnostic_testbusiness.industryHistologyDNAHyperplasiaMiddle Agedmedicine.diseaseFlow CytometryPrognosisUpper aerodigestive tractOtorhinolaryngologyDysplasiaCarcinoma Squamous CellFemalemedicine.symptombusinessORL; journal for oto-rhino-laryngology and its related specialties
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Proteinase-3 mRNA expressed by glomerular epithelial cells correlates with crescent formation in Wegener's granulomatosis

2000

Proteinase-3 mRNA expressed by glomerular epithelial cells correlates with crescent formation in Wegener's granulomatosis. Background Wegener's granulomatosis (WG) is characterized by systemic vasculitis with crescentic glomerulonephritis (CGN) and circulating autoantibodies directed against neutrophil cytoplasmic antigens (ANCA). Proteinase 3 (PR-3), a neutral serine proteinase in neutrophils implicated in the growth control of myeloid cells, has been identified as the target antigen for ANCA in WG. Since the kidneys are frequently involved in WG, we studied the in situ expression of PR-3 by renal parenchymal cells. Methods We assessed the expression of PR-3 in kidney biopsies of 15 patien…

AdultMalePathologymedicine.medical_specialtyBiopsyMyeloblastinKidney GlomerulusIn situ hybridizationBiologyurologic and male genital diseasesKidneyvasculitisAntigenProteinase 3medicineRapidly progressive glomerulonephritisHumanscrescent glomerulonephritisNorthern blotRNA Messengerrapidly progressive glomerulonephritisCells CulturedAgedKidneyANCAurogenital systemSerine EndopeptidasesGranulomatosis with PolyangiitisEpithelial CellsMiddle Agedmedicine.diseasekidney parenchymal cellsmedicine.anatomical_structureKidney TubulesNephrologyImmunohistochemistryFemaleSystemic vasculitisKidney International
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Detection of residual leukemic blasts in adult patients with acute T-lymphoblastic leukemia using bone marrow trephine biopsies: comparison of fluore…

2010

Evaluation of remission in adult acute lymphoblastic leukemia (ALL) normally relies on cytologic evaluation and flow-cytometric analysis. The diagnostic impact of bone marrow trephine biopsies has not been studied so far. We investigated 26 biopsies of 16 patients with T-ALL. Double fluorescent immunostaining with TdT and CD3 was performed. Corresponding cytologic evaluation and flow-cytometric data were available. In 17 of 26 investigations, the results were concordant (either positive or negative). In one examination, residual blasts were not recognized by trephine biopsy, but by other methods. By contrast, in eight investigations, the leukemic blasts were found only in the bone marrow tr…

AdultMalePathologymedicine.medical_specialtyCD3BiopsyCytological TechniquesFluorescent Antibody TechniqueCell SeparationPrecursor T-Cell Lymphoblastic Leukemia-LymphomaSensitivity and SpecificityPathology and Forensic MedicineFlow cytometryYoung AdultBone MarrowCytologyBiopsyMedicineHumansmedicine.diagnostic_testbiologybusiness.industryCell BiologyMiddle AgedFlow CytometryMinimal residual diseaseAdult Acute Lymphoblastic Leukemiabiology.proteinImmunohistochemistryFemalebusinessImmunostainingPathology, research and practice
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Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients

2015

Melanomas are aggressive skin tumors characterized by high metastatic potential. Our previous results indicate that Natural Killer (NK) cells may control growth of melanoma. The main defect of blood NK cells was a decreased expression of activating NCR1/NKp46 receptor and a positive correlation of NKp46 expression with disease outcome in stage IV melanoma patients was found. In addition, in stage III melanoma patients, we identified a new subset of mature NK cells in macro-metastatic Lymph nodes (LN). In the present studies, we evaluated the numbers of NK cells infiltrating primary cutaneous melanoma and analyzed immune cell subsets in a series of sentinel lymph nodes (SLN). First, we show …

AdultMalePathologymedicine.medical_specialtyCD34lcsh:MedicineCD8-Positive T-LymphocytesBiologyTumor MicroenvironmentmedicineHumansCytotoxic T celllcsh:ScienceMelanomaAgedNeoplasm StagingAged 80 and overTumor microenvironmentMultidisciplinarySentinel Lymph Node BiopsyMacrophagesMelanomalcsh:REndothelial CellsMiddle Agedmedicine.diseaseAntigens Differentiation3. Good healthKiller Cells NaturalGranzyme BCutaneous melanomalcsh:QFemaleLymphCD8Research ArticlePLOS ONE
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Evidence for an overlap syndrome of autoimmune hepatitis and primary sclerosing cholangitis.

1996

Abstract Background/Aims: Autoimmune hepatitis, primary biliary cirrhosis and primary sclerosing cholangitis are chronic liver diseases with probable autoimmune background. Overlapping features have been described for primary biliary cirrhosis and autoimmune hepatitis. In contrast, there have been only a few case reports on an overlap of autoimmune hepatitis and primary sclerosing cholangitis. Methods: We describe three male patients with clinical and histological overlapping features of primary sclerosing cholangitis and autoimmune hepatitis. Results: All initially asymptomatic patients had elevated levels of aminotransferases, alkaline phosphatase, γ-glutamyltranspeptidase and IgG. Anti-n…

AdultMalePathologymedicine.medical_specialtyCirrhosisAdolescentBiopsyCholangitis SclerosingAutoimmune hepatitisGastroenterologyPrimary sclerosing cholangitisAutoimmune DiseasesDiagnosis DifferentialPrimary biliary cirrhosisInternal medicinemedicineHumansTransaminasesHepatitis ChronicHepatitisAutoimmune diseaseCholangiopancreatography Endoscopic RetrogradeHepatologyBile ductbusiness.industryOverlap syndromeSyndromemedicine.diseasemedicine.anatomical_structureAntibodies AntinuclearImmunoglobulin GbusinessImmunosuppressive AgentsFollow-Up StudiesJournal of hepatology
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In vivo molecular imaging of gastric cancer by targeting MG7 antigen with confocal laser endomicroscopy.

2013

Background and study aims: In vivo molecular imaging represents a powerful tool for the immediate diagnosis of gastric cancer. In this study, the monoclonal antibody MG7, which is a specific molecular marker against gastric cancer, was labeled with fluorescent agents to enable in vivo real-time imaging by confocal laser endomicroscopy (CLE). Patients and methods: In vivo molecular imaging was performed in tumor-bearing mice from two kinds of human gastric cancer cell lines. Xenograft tumors were visualized in vivo first with a whole-body fluorescent imaging device and then by CLE using fluorescently labeled MG7 antibody. Gastric cancerous tissue and noncancerous mucosa from human biopsies o…

AdultMalePathologymedicine.medical_specialtyConfocalMiceAntigenIn vivoAntigens NeoplasmStomach NeoplasmsCell Line TumorBiopsymedicineAnimalsHumansSingle-Blind MethodAgedAged 80 and overMice Inbred BALB CMicroscopy Confocalmedicine.diagnostic_testbusiness.industryCarcinomaGastroenterologyCancerAntibodies MonoclonalMiddle Agedmedicine.diseaseImmunohistochemistryMolecular ImagingMicroscopy FluorescenceImmunohistochemistryFemaleMolecular imagingbusinessEx vivoEndoscopy
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Oral postinflammatory pigmentation : an analysis of 7 cases

2010

Oral postinflammatory pigmentation (OPP) is a discoloration of the oral mucosa caused by an excess of melanin production and deposition within the basal layer of the epithelium and connective tissue of areas affected by chronic inflammation. Therefore, it is mandatory to demonstrate the association with a previous or concomitant inflammatory process in the same area of oral mucosa. Clinically OPP appears as a localized or diffuse, black to brown pigmentation. OPP may persist for many years even though the disappearing of the pigmentation after the resolution of the inflammatory state has been reported. We reviewed retrospectively the medical records and, when performed, biopsy examinations …

AdultMalePathologymedicine.medical_specialtyConnective tissueMelaninBasal (phylogenetics)BiopsymedicineHumansOral mucosaGeneral DentistryAgedRetrospective StudiesHypopigmentationStomatitisHigh prevalencemedicine.diagnostic_testbusiness.industryMouth MucosaMiddle Agedmedicine.disease:CIENCIAS MÉDICAS [UNESCO]stomatognathic diseasesmedicine.anatomical_structureOtorhinolaryngologyConcomitantUNESCO::CIENCIAS MÉDICASFemaleSurgeryOral lichen planusbusiness
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The Glomerular Lesions in Endotheliotropic Hemolytic Nephroangiopathy (Hemolytic Uremic Syndrome, Malignant Nephrosclerosis, Post Partal Renal Insuff…

1982

The term "Endotheliotropic Hemolytic Nephroangiopathy" (EHN) comprises various clinically or pathomorphologically defined disease states with severe renal lesions (e.g. hemolytic uremic syndrome, malignant nephrosclerosis, post partum renal insufficiency) which, to date, have been considered as different entities. We attempted to assign accompanying glomerular changes based upon light and electron microscopy to the above mentioned clinical pictures and their various stages. The accordingly classified glomerular lesions (G1--G3 and Ga) are of critical importance in pathohistological differential diagnosis. Since it is assumed that fibrin is a causing event in the pathogenesis of the vascular…

AdultMalePathologymedicine.medical_specialtyEndotheliumBiopsyKidney GlomerulusDiseaseFibrinPathology and Forensic MedicinePathogenesisPregnancyGlomerulopathyBiopsymedicineHumansChildAgedFibrinNephrosclerosisbiologymedicine.diagnostic_testMalignant nephrosclerosisbusiness.industryInfantPuerperal DisordersCell BiologyAcute Kidney InjuryMiddle Agedmedicine.diseaseMicroscopy Electronmedicine.anatomical_structureChild PreschoolHemolytic-Uremic Syndromebiology.proteinFemaleDifferential diagnosisbusinessPathology - Research and Practice
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Value of videoroscopy in the detection of alterations of  Actinic Cheilitis and the selection of biopsy areas

2015

Background To demonstrate the value of videoroscopy in identifying lesions and alterations not seen by oroscopy and to select the area for biopsy. Material and Methods Eighty patients were subjected to anamnesis, physical exam, videoroscopy exam, toluidine blue test and biopsy. A diagram of the lips was created to record the exact location where the lesion was found. Results Physical exam identified 287 lesions, and videoroscopy identified 587 lesions; erythema and white lesions were the most common lesions associated with actinic cheilitis. Of the 59 performed biopsies, 32 (52.4%) cases were identified by videoroscopy that showed lesions that were not detected during physical examination. …

AdultMalePathologymedicine.medical_specialtyErythemaAdolescentBiopsyVideo RecordingOdontologíaPhysical examinationLesionYoung AdultBiopsyMedicineHumansExact locationOral DiagnosisChildGeneral DentistryAgedAged 80 and overOral Medicine and Pathologymedicine.diagnostic_testbusiness.industryActinic cheilitisResearchDiagnosis OralMiddle Aged:CIENCIAS MÉDICAS [UNESCO]medicine.diseaseCiencias de la saludDermatologyLipOtorhinolaryngologyCheilitisUNESCO::CIENCIAS MÉDICASSurgeryPhysical examFemalemedicine.symptombusinessMedicina Oral, Patología Oral y Cirugía Bucal
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Image cytometric DNA analysis of mucosal biopsies in patients with primary achalasia.

2006

(P < 0.0001), and 9c- (P = 0.0001) exceeding rate with progredient DNA alterations in the respective order. CONCLUSION: The finding that DNA aneuploidy was identifi ed by image cytometry in esophageal specimens of patients with achalasia, which may be due to specifi c chromosomal alterations presenting as precancerous lesions in 27% of patients, leads us to conclude that image cytometry represents a valuable screening tool. Abstract AIM: To determine DNA aneuploidy in mucosal biopsies of achalasia patients for subsequent rapid diagnosis. METHODS: Biopsies from the middle third of the esophagus were obtained in 15 patients with achalasia. Immunohistochemical staining was carried out with mon…

AdultMalePathologymedicine.medical_specialtyEsophageal NeoplasmsBiopsyAchalasiaAneuploidyBiologydigestive systemBiopsyotorhinolaryngologic diseasesCarcinomamedicineHumansGenetic TestingFeulgen stainEsophagusAgedImage CytometryMucous Membranemedicine.diagnostic_testGastroenterologyDNAGeneral MedicineMiddle AgedAneuploidymedicine.diseaseImmunohistochemistrydigestive system diseasesEsophageal AchalasiaBasic ResearchKi-67 Antigenmedicine.anatomical_structureDysplasiaCarcinoma Squamous CellImage CytometryFemaleTumor Suppressor Protein p53Precancerous Conditions
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