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showing 10 items of 268 documents

Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome

2004

A bacterial artificial chromosomal library of Nonomuraea sp. ATCC39727 was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of dbv genes known to be involved in the biosynthesis of the glycopeptide A40926. dbv genes were cloned as two large, partially overlapping, fragments and transferred into the host Streptomyces lividans, thus generating strains S. lividansColon, two colonsNmESAC50 and S. lividansColon, two colonsNmESAC57. The heterologous expression of Nonomuraea genes in S. lividans was successfully demonstrated by using combined RT-PCR and proteomic approaches. MALDI-TOF analysis revealed that a Nonomuraea ABC transporter is e…

DNA BacterialChromosomal library of Nonomuraea sp. ATCC39727Escherichia coli–Streptomyces artificial chromosome (ESAC)RT-PCRMolecular cloningApplied Microbiology and BiotechnologyStreptomycesGenetic analysisThiostreptonchemistry.chemical_compoundActinomycetalesChromosomes ArtificialCloning MolecularA40926GeneRegulator geneGeneticsGenomic LibrarybiologyMALDI-TOF mass spectrometryPromoterGeneral Medicinebiology.organism_classificationStreptomycesdbv gene cluster2D-PAGEchemistryGenes BacterialHeterologous expressionHeterologous expressionPulsed field gel electrophoresidalbavancinBiotechnologyApplied Microbiology and Biotechnology
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Business Constraints Integration in a Search Engine Optimization Fuzzy Decision Support System

2017

The Internet has dramatically changed the way companies can reach their clients. Henceforth, to develop their business online, companies have to drive qualified traffic to their website. For this, their website must be visible in the first pages of search engine results. Search Engine Optimization (SEO) permits the improvement of the position of a website on the search engines results pages. However, the practice of SEO is complex, and results are uncertain, because search engines do not communicate much about their ranking methods. Moreover, this activity takes time, and requires the implementation of modifications that can alter the visual appearance of the webpages. This paper proposes a…

Decision support systemEngineeringbusiness.industryProcess (engineering)02 engineering and technologyDecision ruleRanking (information retrieval)World Wide WebSearch engine020204 information systemsSearch engine optimizationWeb page0202 electrical engineering electronic engineering information engineering020201 artificial intelligence & image processingThe Internetbusiness2017 IEEE 19th Conference on Business Informatics (CBI)
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Identification of picobirnavirus from faeces of Italian children suffering from acute diarrhea

1996

Polyacrylamide gel electrophoresis of nucleic acid extracted from stool samples of diarrhoeic children revealed in 3 out of 690 (0.43 %) specimens two electrophoretic bands with a migration pattern characteristic of picobirnavirus ds-RNA. In none of the 92 control children were similar bands detected. No other potential enteric pathogens were found in the patients with picobirnavirus infection.

DiarrheaMaleAcute diarrheaEpidemiologyPicobirnavirusMicrobiologyFecesPicobirnavirus Gastroenteritis PAGEHumansMedicineChildPolyacrylamide gel electrophoresisPicobirnavirusFecesGel electrophoresisbiologybusiness.industryBrief Reportbiology.organism_classificationGastroenteritisPAGEDiarrheaItalyVirus DiseasesRNA ViralElectrophoresis Polyacrylamide GelFemaleViral diseasemedicine.symptombusinessEuropean Journal of Epidemiology
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Querying Dynamic and Context-Sensitive Metadata in Semantic Web

2005

RDF (core Semantic Web standard) is not originally appropriate for context representation, because of its initial focus on the ordinary Web resources, such as web pages, files, databases, services, etc., which structure and content are more or less stable. However, on the other hand, emerging industrial applications consider e.g. machines, processes, personnel, services for condition monitoring, remote diagnostics and maintenance, etc. to be specific classes of Web resources and thus a subject for semantic annotation. Such resources are naturally dynamic, not only from the point of view of changing values for some attributes (state of resource), but also from the point of view of changing “…

Document Structure DescriptionWeb standardsmedicine.medical_specialtyWeb developmentWeb 2.0Computer scienceRDF Schemacomputer.software_genreSocial Semantic WebWorld Wide WebAnnotationWeb pagemedicineSemantic analyticsSPARQLSemantic Web StackRDFCwmSemantic WebData Webbusiness.industrySemantic Web Rule Languagecomputer.file_formatLinked dataMetadataSemantic gridSimple Knowledge Organization SystemThe InternetWeb serviceWeb resourcebusinesscomputerWeb modeling
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Seepage carbonate mounds in Cenozoic sedimentary sequences from the Las Minas Basin, SE Spain

2016

Abstract A number of carbonate mounds composed of indurate, strongly folded and/or brecciated calcite and dolomite beds occur interstratified in Cenozoic sedimentary sequences from the Las Minas Basin. Part of the fabric of the rock forming the carbonate mounds is composed of laminated to banded dolostone similar to the host rock but showing contrasted lithification. Moreover, the carbonate deposits of the mounds display aggrading neomorphism of dolomite, partial replacement of dolomite by calcite, calcite cementation, and extensive silicification, locally resulting in box-work fabric. Eight main lithofacies were distinguished in the carbonate mound deposits. In some lithofacies, chert is p…

DolostoneCalcite010506 paleontologyStratigraphySE SpainDolomiteGeochemistryPetrologíaGeologyartesian groundwaterNeomorphism010502 geochemistry & geophysics01 natural scienceschemistry.chemical_compoundchemistryCarbonate moundCarbonate rockCarbonateSedimentary rockseepage chertLithificationGeologycenozoic0105 earth and related environmental sciences
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La spécificité du sport menacée ?

2007

International audience

EDUCATION PHYSIQUE ET SPORTS[SHS.DROIT]Humanities and Social Sciences/LawDopage[SHS.DROIT] Humanities and Social Sciences/LawCIORépressionComputingMilieux_MISCELLANEOUSRèglement anti-dopage
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Influence of online transparency on efficiency. Analysis of spanish NGDOs

2020

This study examines (a) whether nongovernmental development organizations (NGDOs) disseminate relevant information for their stakeholders through their web pages, information that after being reviewed and evaluated by external organizations such as the Spanish Coordinator of Development NGO or Lealtad Foundation, allowed these NGDOs to obtain a seal of transparency and (b) whether their level of transparency influences efficiency. To determine online transparency, web pages of seal-approved NGDOs were reviewed to assess the availability of relevant information. This paper uses data envelopment analysis to assess the efficiency using an input orientation. To determine the influence of online…

Economics and EconometricsKnowledge managementSociology and Political Sciencebusiness.industryBest practiceUNESCO::CIENCIAS ECONÓMICAS:CIENCIAS ECONÓMICAS [UNESCO]Transparency (behavior)Competition (economics)Web pageData envelopment analysisNonprofit sectorbusinessRelevant informationDisseminationCIRIEC-España, revista de economía pública, social y cooperativa
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A Data-Driven Approach to Dynamically Learn Focused Lexicons for Recognizing Emotions in Social Network Streams

2016

Opinion Mining aims at identifying and classifying subjective information in a collection of documents. A variety of approach exists in literature, ranging from Supervised Learning to Unsupervised Learning. Currently, one of the biggest opinion resource of opinionated texts existing on the Web is represented by Social Networks. Networks are not only a vast collection of documents but they also represent a dynamic evolving resource as the users keep posting their own opinions. We based our work relying on this idea of dynamicity, building an evolving model that updates itself in real time as users submit their posts. This is done through a set of supervised techniques based on a Lexi- con of…

Emotion AnalysisSocial networkbusiness.industrymedia_common.quotation_subjectSentiment analysisSupervised learningDynamic web pageWorld Wide WebSadnessSurpriseResource (project management)Social NetworksUnsupervised learningData-driven modelsArtificial intelligencebusinessPsychologymedia_common
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CATCOM: A Database on Performances of Spanish Classical Theater

2013

The research group DICAT (http://www.uv.es/dicat/index_en.html) has been working for almost twenty years on the creation of research instruments that will enhance scholars' knowledge of Early Modern Spanish theater through the use of digital tools that create new ways to combine and apply data.1 The database Diccionario biografico de actores del teatro clasico espanol (DICAT) (Ferrer Valls) was the result of work done during nearly fifteen years. The DICAT group created a database with almost five thousand entries, which document the activity of actors, actresses, heads of companies, and musicians who worked in Spanish professional theatrical companies during the sixteenth and seventeenth c…

EngineeringDatabasePoint (typography)business.industrySection (typography)TeatreSpace (commercial competition)computer.software_genreLiteratura castellana 1500-1700 Segle d'OrDocumentationIndex (publishing)Management of Technology and InnovationEarly modern periodWeb pageSelection (linguistics)businesscomputerJournal for Early Modern Cultural Studies
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Modeling Round Robin Test: An Uncoupled Approach

2014

Abstract The solution of the modeling test presented in the paper is based on an uncoupled hydro-mechanical approach. Firstly, the controlled infiltration process is modeled by a finite element transient groundwater seepage software. Afterwards, calculated pore water pressures at successive instants are used for the slope stability analysis. Time evolution of the slope stability is analysed by using the infinite slope model, according to the classical limit equilibrium method.

EngineeringSettore ICAR/07 - Geotecnicainfinite slopebusiness.industryTime evolutionEarth and Planetary Sciences(all)FEM seepage analysisGeneral MedicineMechanicsClassical limitFinite element methodPhysics::GeophysicsMELPore water pressureInfiltration (hydrology)FEM seepage analysiinfinite slope.Slope stabilityGeotechnical engineeringRound robin testbusinessSlope stability analysisunsaturated soilProcedia Earth and Planetary Science
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