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showing 10 items of 9912 documents

SARS-CoV2 vertical transmission with adverse effects on the newborn revealed through integrated immunohistochemical, electron microscopy and molecula…

2020

Background: The occurrence of trans-placental transmission of severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) infection remains highly debated. Placental positivity for SARS-CoV-2 has been reported in selected cases, but infection or virus-associated disease of fetal tissues or newborns remains to be demonstrated. Methods: We screened for SARS-CoV-2 spike (S) protein expression placentas from 101 women who delivered between February 7 and May 15 2020, including 15 tested positive for SARS-CoV-2 RNA, 34 tested negative, and 52 not evaluated as they did not meet testing criteria (32), or delivered before COVID-19 pandemic declaration (20). Immunostain for SARS-CoV-2 nucleocapsid…

0301 basic medicinePathologyCOVID19Placentaviruseslcsh:MedicineExtracellular Traps0302 clinical medicinePregnancyNasopharynxPathology MolecularPregnancy Complications InfectiousAdult Betacoronavirus COVID-19 Coronavirus Infections Coronavirus Nucleocapsid Proteins Female Humans Immunohistochemistry Infant Newborn Spike Glycoprotein Coronavirus Microscopy Electron Nasopharynx PregnancySpike Glycoprotein CoronavirusSARS-CoV-2lcsh:R5-920medicine.diagnostic_testIntervillous spaceGeneral MedicineNucleocapsid ProteinsImmunohistochemistrymedicine.anatomical_structure030220 oncology & carcinogenesisSpike Glycoprotein CoronavirusRNA ViralFemaleCoronavirus Infectionslcsh:Medicine (General)Adultmedicine.medical_specialtyPneumonia ViralIn situ hybridizationSettore MED/08 - Anatomia PatologicaBiologyImmunofluorescenceArticleGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesBetacoronavirusSyncytiotrophoblastImmune systemAntigenPlacentamedicineSettore MED/05 - Patologia ClinicaCoronavirus Nucleocapsid ProteinsHumansPandemicsPregnancyFetusbusiness.industrySARS-CoV-2Macrophageslcsh:RInfant NewbornCOVID-19medicine.diseasePhosphoproteinsInfectious Disease Transmission VerticalMicroscopy Electron030104 developmental biologybusinessEBioMedicine
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Interleukin-9 Overexpression and Th9 Polarization Characterize the Inflamed Gut, the Synovial Tissue, and the Peripheral Blood of Patients With Psori…

2016

Objective To investigate the expression and tissue distribution of Th9-related cytokines in patients with psoriatic arthritis (PsA). Methods Quantitative gene expression analysis of Th1, Th17, and Th9 cytokines was performed in intestinal biopsy samples obtained from patients with PsA, HLA-B27-positive patients with ankylosing spondylitis (AS), patients with Crohn's disease (CD), and healthy controls. Expression and tissue distribution of interleukin-23 (IL-23), IL-17, IL-22, IL-9, and IL-9 receptor (IL-9R) were evaluated by immunohistochemistry and confocal microscopy. Flow cytometry was used to study the frequency of Th9 cells among peripheral blood, lamina propria, and synovial fluid mon…

0301 basic medicinePathologymedicine.medical_specialtyImmunologyHigh endothelial venulesArthritisInflammationPeripheral blood mononuclear cell03 medical and health sciences0302 clinical medicineRheumatologymedicineImmunology and AllergyInterleukin 9030203 arthritis & rheumatologyLamina propriabusiness.industrymedicine.disease3. Good health030104 developmental biologymedicine.anatomical_structureImmunologyPaneth cellmedicine.symptomSynovial membranebusinessArthritis & Rheumatology
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Identification of a tumor-reactive T-cell repertoire in the immune infiltrate of patients with resectable pancreatic ductal adenocarcinoma

2016

The devastating prognosis of patients with resectable pancreatic ductal adenocarcinoma (PDA) presents an urgent need for the development of therapeutic strategies targeting disseminated tumor cells. Until now, T-cell therapy has been scarcely pursued in PDA, due to the prevailing view that it represents a poorly immunogenic tumor.We systematically analyzed T-cell infiltrates in tumor biopsies from 127 patients with resectable PDA by means of immunohistochemistry, flow cytometry, T-cell receptor (TCR) deep-sequencing and functional analysis ofProminent T-cell infiltrates, as well as tertiary lymphoid structures harboring proliferating T-cells, were detected in the vast majority of biopsies f…

0301 basic medicinePathologymedicine.medical_specialtyT cell repertoirePancreatic ductal adenocarcinomaTertiary Lymphoid StructuresTumor-infiltrating lymphocyteseducationImmunologyBiology03 medical and health sciences030104 developmental biology0302 clinical medicineOncology030220 oncology & carcinogenesisMIATA Compliant Research PapermedicineImmunology and AllergyImmune infiltrateOncoImmunology
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Jacobsen syndrome and neonatal bleeding: report on two unrelated patients

2021

Abstract Introduction In 1973, Petrea Jacobsen described the first patient showing dysmorphic features, developmental delay and congenital heart disease (atrial and ventricular septal defect) associated to a 11q deletion, inherited from the father. Since then, more than 200 patients have been reported, and the chromosomal critical region responsible for this contiguous gene disorder has been identified. Patients’ presentation We report on two unrelated newborns observed in Italy affected by Jacobsen syndrome (JBS, also known as 11q23 deletion). Both patients presented prenatal and postnatal bleeding, growth and developmental delay, craniofacial dysmorphisms, multiple congenital anomalies, a…

0301 basic medicinePediatricsmedicine.medical_specialtyGenotype-phenotype correlationHeart diseaseGenetic counselingCase ReportIn situ hybridization030105 genetics & heredityPediatricsRJ1-57003 medical and health sciences0302 clinical medicineaCGHJBSmedicineHumansJacobsen Distal 11q Deletion SyndromeJacobsen syndromeCraniofacialGenetic Association StudiesCerebral Hemorrhage11q23 deletionbusiness.industryInfant NewbornEarly diagnosimedicine.diseaseEarly diagnosisPancytopeniaThrombocytopeniaItalyFemalePresentation (obstetrics)business030217 neurology & neurosurgeryComparative genomic hybridizationItalian Journal of Pediatrics
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Dental status, salivary flow, and sociodemographic aspects in Sheehan Syndrome patients

2018

Background Sheehan’s syndrome (SS) is one of the leading causes of hypopituitarism in developing countries. It occurs after postpartum necrosis of the pituitary gland, and it is considered a significant public health problem. This paper, apparently unpublished, aimed to perform an analysis on oral aspects in patients with SS. Material and Methods A cross-sectional study was performed with 23 women diagnosed with SS at the Division of Endocrinology and Diabetes (Walter Cantídio University Hospital, Fortaleza, Brazil). Results Data on sociodemographic, dental and salivary flow aspects were collected through a clinical approach and a panoramic radiograph request. The mean age was 64 ± 11.5 yea…

0301 basic medicinePediatricsmedicine.medical_specialtyPanoramic radiographMaxillary sinus030209 endocrinology & metabolismHypopituitarismXerostomiaHypopituitarismTooth Loss03 medical and health sciences0302 clinical medicineDiabetes mellitusHumansMedicineGeneral DentistryAgedOral Medicine and Pathologybusiness.industryResearchPublic healthMiddle Aged:CIENCIAS MÉDICAS [UNESCO]University hospitalmedicine.diseaseSheehan SyndromeCross-Sectional Studiesmedicine.anatomical_structureSocioeconomic FactorsOtorhinolaryngologyUNESCO::CIENCIAS MÉDICASHipopituitarismoFemaleSurgery030101 anatomy & morphologybusinessOral medicineBrazilMedicina Oral Patología Oral y Cirugia Bucal
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Socioeconomic factors and severity of periodontal disease in adults (35-44 years). A cross sectional study

2017

Background Periodontal disease or periodontitis is an inflammatory disease with a hight prevalence. According to the last oral health survey of the Spanish population, between 24% and 37% of Spaniards aged over 35 years have periodontitis and 6% to 10% of the adult population have deep periodontal pockets. The aim of this study was to determine the association between risk factors and the presence of periodontal pockets in the adult population. Material and methods A cross sectional or prevalence study of a representative sample of the adult population of the Valencia region was designed. The sample was recruited at 35 health centres, The study was conducted in November and December 2006 un…

0301 basic medicinePeriodontitisGingival and periodontal pocketCross-sectional studybusiness.industryResearch030206 dentistryDiseasemedicine.diseaseSocial class:CIENCIAS MÉDICAS [UNESCO]Community and Preventive DentistrySpanish population03 medical and health sciences030104 developmental biology0302 clinical medicinePeriodontal diseaseUNESCO::CIENCIAS MÉDICASmedicinebusinessGeneral DentistrySocioeconomic statusDemographyJournal of Clinical and Experimental Dentistry
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European countries in the race to attract successful biopharma investment: Winners and laggers

2021

The post-coronavirus era will open myriad opportunities for the biopharma industry. However, the extent to which each country will take advantage of this promising new scenario will largely depend on its position in a few key areas. Here, we offer an overview of the European countries that are winning and those that are lagging behind in the race to attract the greatest investment in this industry and to attain the highest rate of successful new ventures. Our results highlight the vital importance of a sound, active funding base, especially in terms of venture capital. Our findings also suggests that general scientific foundations are not enough to secure an advantage in new venture formati…

0301 basic medicinePharmacologyDrug IndustryResearchCOVID-19New VenturesVenture capitalInvestment (macroeconomics)Europe03 medical and health sciencesRace (biology)030104 developmental biology0302 clinical medicineMarket economyDrug Development030220 oncology & carcinogenesisDrug DiscoveryPosition (finance)BusinessInvestmentsLaggingPandemicsFoundationsDrug Discovery Today
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Stochastic sampling effects favor manual over digital contact tracing.

2020

Isolation of symptomatic individuals, tracing and testing of their nonsymptomatic contacts are fundamental strategies for mitigating the current COVID-19 pandemic. The breaking of contagion chains relies on two complementary strategies: manual reconstruction of contacts based on interviews and a digital (app-based) privacy-preserving contact tracing. We compare their effectiveness using model parameters tailored to describe SARS-CoV-2 diffusion within the activity-driven model, a general empirically validated framework for network dynamics. We show that, even for equal probability of tracing a contact, manual tracing robustly performs better than the digital protocol, also taking into accou…

0301 basic medicinePhysics - Physics and SocietyComputer scienceEpidemiologyScienceComplex networksFOS: Physical sciencesGeneral Physics and AstronomyPhysics and Society (physics.soc-ph)Tracingcomputer.software_genreGeneral Biochemistry Genetics and Molecular BiologyArticleSpecimen Handling03 medical and health sciences0302 clinical medicineHumans030212 general & internal medicineQuantitative Biology - Populations and EvolutionPandemicsCondensed Matter - Statistical Mechanicsstochastic modelProtocol (science)Stochastic ProcessesMultidisciplinaryStatistical Mechanics (cond-mat.stat-mech)Stochastic processDiagnostic Tests RoutineSARS-CoV-2QPopulations and Evolution (q-bio.PE)Sampling (statistics)COVID-19General ChemistryComplex networkModels TheoreticalNetwork dynamics030104 developmental biologyFOS: Biological sciencesScalabilityQuarantineData miningContact TracingcomputerContact tracingAlgorithmsNature communications
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Molecular aspects of pancreatic β-cell dysfunction: Oxidative stress, microRNA, and long noncoding RNA.

2018

Metabolic syndrome is known as a frequent precursor of type 2 diabetes mellitus (T2D). This disease could affect 8% of the people worldwide. Given that pancreatic β-cell dysfunction and loss have central roles in the initiation and progression of the disease, the understanding of cellular and molecular pathways associated with pancreatic β-cell dysfunction can provide more information about the underlying pathways involved in T2D. Multiple lines evidence indicated that oxidative stress, microRNA, and long noncoding RNA play significant roles in various steps of diseases. Oxidative stress is one of the important factors involved in T2D pathogenesis. This could affect the function and surviva…

0301 basic medicinePhysiologyClinical BiochemistryCellDiseaseBiologymedicine.disease_causePathogenesis03 medical and health sciences0302 clinical medicineInsulin-Secreting CellsGene expressionmicroRNAmedicineHumansEpigeneticsPancreasCell BiologyLong non-coding RNACell biologyMicroRNAsOxidative Stress030104 developmental biologymedicine.anatomical_structureDiabetes Mellitus Type 2030220 oncology & carcinogenesisRNA Long NoncodingOxidative stressSignal TransductionJournal of cellular physiology
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The activity of Artemisia spp. and their constituents against Trypanosomiasis.

2018

Abstract Background Trypanosomiasis belongs to the neglected tropical diseases. Although standard therapies are available, the safety and efficacy of current synthetic drugs are limited due to the development of drug resistance and adverse side effects. Purpose Artemisia annua and artemisinin are not only active against Plasmodia, but also other protozoa. Therefore, we reviewed the literature on species of the genus Artemisia and their phytochemicals regarding their activity against trypanosomes. Study design A PubMed search for “Artemisia/Artemisinin and Trypanosoma” has been conducted for literature until December 2017. Results Interestingly, not only A. annua L. and its active principle,…

0301 basic medicinePhytochemicalsArtemisia annuaPharmaceutical ScienceArtesunateBiologyAbsinthium03 medical and health scienceschemistry.chemical_compoundAntimalarials0302 clinical medicineTrypanosomiasisScopoletinparasitic diseasesDrug DiscoverymedicineOils VolatileArtemetherArtemisininPharmacologyScopoletinTraditional medicinePlant Extractsfood and beveragesbiology.organism_classificationArtemisinins030104 developmental biologyComplementary and alternative medicinechemistryArtemisiaArtesunate030220 oncology & carcinogenesisTrypanosomaMolecular MedicineArtemisiaArtemethermedicine.drugPhytomedicine : international journal of phytotherapy and phytopharmacology
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