Search results for "pancrea"
showing 4 items of 814 documents
MTOR inhibitor-based combination therapies for pancreatic cancer
2018
Background: Although the mechanistic target of rapamycin (MTOR) kinase, included in the mTORC1 and mTORC2 signalling hubs, has been demonstrated to be active in a significant fraction of patients with pancreatic ductal adenocarcinoma (PDAC), the value of the kinase as a therapeutic target needs further clarification. Methods: We used Mtor floxed mice to analyse the function of the kinase in context of the pancreas at the genetic level. Using a dual-recombinase system, which is based on the flippase-FRT (Flp-FRT) and Cre-loxP recombination technologies, we generated a novel cellular model, allowing the genetic analysis of MTOR functions in tumour maintenance. Cross-species validation and pha…
Estrés por disulfuro en pancreatitis aguda experimental. Implicaciones fisiopatológicas
2014
El estrés oxidativo se define como la alteración en el equilibrio entre las especies oxidantes y antioxidantes, a favor de las primeras. Mientras que hay muchos tipos de daño oxidativo, cada vez más pruebas sugieren un papel importante en la oxidación de proteínas en múltiples enfermedades. Los puentes disulfuro se forman en el citosol durante el estrés oxidativo y funcionan como intercambiadores redox. El glutatión es un tripéptido y es uno de los principales antioxidantes, lo que le confiere un papel clave en numerosas funciones celulares. El estado redox del glutatión se considera el mejor indicador de estrés oxidativo en las células, ya que refleja el equilibrio entre el estado antioxid…
CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreati…
2021
Coxsackievirus B3 (CVB3) belongs to the genus Enterovirus of the family Picornaviridae and can cause acute acinar pancreatitis in adults. However, the molecular mechanisms of pathogenesis underlying CVB3-induced acute pancreatitis have remained unclear. In this study, we discovered that CVB3 capsid protein VP1 inhibited pancreatic cell proliferation and exerted strong cytopathic effects on HPAC cells. Through yeast two-hybrid, co-immunoprecipitation, and confocal microscopy, we show that Menage a trois 1 (MAT1), a subunit of the Cdk-Activating Kinase (CAK) complex involved in cell proliferation and transcription, is a novel interaction protein with CVB3 VP1. Moreover, CVB3 VP1 inhibited MAT…