Search results for "pcr"
showing 10 items of 438 documents
Preliminary evaluation of a zinc-copper-citric acid biocomplex for the control of Plenodomus tracheiphilus causal agent of citrus mal secco disease
2022
In this study we evaluated a zinc copper-citric acid biocomplex (Dentamet®) for the effect on citrus mal secco disease caused by Plenodomus tracheiphilus. In vitro tests highlighted an inhibitory activity of the compound on mycelial growth and phialoconidia germination. Independent in vivo trials were performed in a growth chamber inoculating a virulent fungal isolate on sour orange seedlings and young plants of two lines of Femminello lemon, namely ‘Zagara bianca’ and ‘2Kr’, grafted on sour orange and grown in pots. The compound was leaf sprayed three days before the inoculation by wounds with phialoconidia. Overall, disease index and disease progress curve in plants inoculated with P. tra…
A new method for detecting viruses that cause tomato yellow leaf curl disease in the Mediterranean region and their recombinants.
2007
CARATTERIZZAZIONE MOLECOLARE DI BATTERI IN REPERTI LIGNEI SOMMERSI
2013
The focus of this study was the identification of bacterial colonies in waterlogged wood fragments from the rostrum of a excellent workmanship, that is very likely one of the wrecks attributed to Sextus Pompey fleet (36 BC) and discovered in Acqualadroni, Messina, Sicily, Italy (2008). The wood samples were analyzed by light and Scanning Electron Microscopy (SEM), in vitro culture and molecular technique (DNA base techniques). The results, focused on bacterial consortia, allowed us to reveal the presence of Pseudomonas sp., Sphingomonas sp., Xanthomonas sp. besides Marinobacter sp. and Desulforudis audaxviator. A prompt and accurate characterization of bacterial colonization represents one …
Are control of extracellular acid-base balance and regulation of skeleton genes linked to resistance to ocean acidification in adult sea urchins?
2020
SCOPUS: ar.j
Expression of cytokeratins in human umbilical cord at term
2009
Background. Cytokeratins are the proteins that costitute intermediate filaments. In the cytoplasm, keratin filaments extend from the nucleus to the plasma membrane. This link is important for the structural organization of the cytoplasm and for many cellular processes such as mitosis, cell movement, cell differentiation and adhesion. The pattern of expression of cytokeratins is frequently organ or tissue specific and depends mainly on the type of epithelium, the level of differentiation and the stage of development. The expression of cytokeratins in umbilical cord has been investigated by many groups, but these previous studies gave only fragmentary informations: each single study focused o…
Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples
2008
Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…
Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa
2009
The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…
Matrix metalloproteinase-2 matrix metalloproteinase-9 and inducible nitric oxide synthase in oral leukoplakia: Immunohistochemistry and RT-PCR analys…
2008
Oral leukoplakia is the most common and potentially malignant disorder of the oral mucosa. The definition of leukoplakia given by the World Health Organization is "a white plaque that cannot be characterized either from a clinical or from a histopathological point of view", thus the diagnosis of leukoplakia is based on the exclusion of other lesions of the oral mucosa. We believe it is necessary to identify molecular and immunohistochemical parameters that can contribute to discriminating between the different leukoplakia clinical subtypes coded by the epidemiology. In the present work we show the preliminary results of this research project. We investigated the expression of matrix metallo…
Metilazione del DNA in artrite reumatoide
2005
Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…