Search results for "pcr"
showing 10 items of 438 documents
Intraspecific biodiversity and 'spoilage potential' of Brettanomyces bruxellensis in Apulian wines
2015
Abstract The yeast Brettanomyces bruxellensis, generally considered the main oenological spoilage microbe, is able to survive during the winemaking process and it confers off-odors to wine, in reason of its ability to produce considerable amounts of volatile phenols. Forty-eight isolates of B. bruxellensis, obtained from several wines collected in Apulia (Southern Italy), were genetically characterized using an integrated approach, including a strain biodiversity analysis by Sau-PCR. Furthermore, the production of volatile phenols was assessed in wine and in synthetic medium, confirming the oenological spoilage potential of the analysed strains. Our findings indicate a remarkable genetic va…
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality -- Method to directly extract DNA from soil samples"
2011
International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …
Determinants of the distribution of nitrogen-cycling microbial communities at the landscape-scale
2010
Little information is available regarding the landscape-scale distribution of microbial communities and its environmental determinants. However, a landscape perspective is needed to understand the relative importance of local and regional factors and land management for the microbial communities. In this manuscript, we investigated the distribution of functional microbial communities involved in N-cycling and of the total bacterial and crenarchaeal communities over 107 sites using a grid with a 16 km lag distance within Burgundy, a 31 500 km2 region in France. After quantifying the abundances of the total bacterial, crenarchaeal, nitrate-reducing, denitrifying and ammonia-oxidizing communit…
Biological nitrogen fixation for the 21st century
1998
The biodiversity of nitrogen-fixing organisms is huge. Taxonomic and phylogenetic research is needed to structure this diversity, to facilitate communication among scientists, and to increase our understanding of the evolution and biology of diazotrophs. Molecular tools for taxonomic and biodiversity studies of diazotrophic rhizobia, frankiae, cyanobacteria and bacilli are presented in sections 2 to 5. Sections 6 to 9 focus on problems with genus and species assignment.
Mapping field-scale spatial distribution patterns of size and activity of the denitrifier community
2008
International audience
Prevalence of Mycobacterium bovis in agroecosystems : analysis of potential environmental reservoirs (soil, fresh water, soil fauna and aquatic fauna…
2016
Bovine tuberculosis is an infectious disease caused by Mycobacterium bovis. This disease affects cattle, and many species of domestic and wild mammals, and humans. The circulation of the bacteria in various multi-host systems promotes the maintenance of the disease and the contamination of cattle in the vicinity. Beside direct transmission of the bacteria through the respiratory route, indirect transmission, through inhalation or ingestion of environmental matrices contaminated by an infected animal excretory, is suspected in several countries. Environmental contamination with M. bovis appears to be a crucial factor in the persistence of the infection in multi-host systems. In Côte d'Or, a …
NF ISO 14239 - Qualité du Sol - Systèmes d’incubation de laboratoire destinés à la mesure de la minéralisation de produits chimiques organiques dans …
2017
EA BIOME INRA; Le présent document spécifie les étapes principales d’une méthode d’amplification par réaction de polymérisation en chaîne (PCR) quantitative (qPCR) permettant de mesurer Domaine d'application Le présent document définit six systèmes d'incubation appropriés permettant de mesurer les vitesses et l'étendue de la minéralisation de composés organiques dans le sol par mesurage du dégagement de dioxyde de carbone (CO2) . Tous les systèmes d'incubation peuvent être utilisés avec des composés solubles ou insolubles, mais le choix du système dépend des objectifs globaux de l'étude. Le présent document ne s'applique pas à l'utilisation desdits systèmes pour les bilans de masse, qui son…
Fungal ecology on grapes
2010
Microbial population of grapes is important from a technological point of view because it determines the quality of wine. But few studies have focused on fungal populations of grapes. A better knowledge of the fungal diversity on grapes, particularly as concerns species responsible for wine defects, may help efforts to control their development.We report the development of a PCR ITS-RFLP method as a fast and easy technique for identifying species of fungal genera present on grapes. By this methode, 41 different fungal species among 43 studied species belonging to 11 genera were characterized at the species level. Only P. thomii remained indistinguishable from P. glabrum. Using this PCR-ITS-…
Ecological role of mycotoxins produced by Fusarium graminearum : consequences of the presence of deoxynivalenol (DON) in crop residues on the soil mi…
2012
Fusarium graminearum is a plant pathogenic fungus, causing devastating disease “Fusarium head blight” (FHB) in cereals including wheat and maize. It also contaminates the grains with mycotoxins including deoxynivalenol (DON) which are toxic to human and animals. This disease has resulted in the serious losses in grain yield and quality. We established through a first bibliographic review that during off season fungus survives saprophytically on the crop residues (ecological habitat) and serves as primary inoculum for the next season crop. However, we noticed also that the literature was poor about the role mycotoxins could play in the establishment of F. graminearum in such a habitat. The m…
Microalgae community structure analysis based on 18S rDNA amplification from DNA extracted directly from soil as a potential soil bioindicator
2005
International audience; Soil algae are photosynthetically active microorganisms showing changeable community structure, depending on the soil type, the agricultural practices and the application of pesticides. To characterise algal community structure, molecular approaches complementary to classical microbiological approaches based on the isolation and the culture of soil algae are required. Our study describes a polymerase chain reaction (PCR) approach targeting algal 18S rDNA sequences of desoxyribonucleic acid (DNA) samples extracted either from unialgal eukaryotic microalgae culture, complex assemblages of microalgae populations or natural soil communities. Our first results showed that…