Search results for "pea"

showing 10 items of 6599 documents

Pearl grafting: tracking the biological origin of nuclei by straightforward immunological methods.

2018

9 pages; International audience; French Polynesia is renowned for the production of Tahitian black pearl. These gems are obtained by grafting a nucleus into the gonad of a receiving oyster together with a graft, i.e. a small section of mantle tissue of a donor oyster. This procedure initiates the formation of a pearl sack around the nucleus, and subsequently, the deposition of concentric layers of nacre. The nucleus plays a key-role in pearl formation and its characteristics influence markedly the quality of the final product. As it is manufactured from mollusc shells, it contains a small percentage of organics. In the present paper, we used a set of biochemical techniques to characterize a…

0106 biological sciences0301 basic medicineOystermatrix proteinsAquatic Science01 natural sciences[ CHIM ] Chemical SciencesPinctada margaritifera03 medical and health sciences[ CHIM.ORGA ] Chemical Sciences/Organic chemistrybiology.animal[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Botanymedicinebiology010604 marine biology & hydrobiologyPearl oysterPinctada margaritiferanucleusAmblemaMusselbiology.organism_classificationAmblema plicataAmblema plicata030104 developmental biologymedicine.anatomical_structureBiochemistry[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ELISApearl oysterNucleusPinctada
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Impact of Bacterial Siderophores on Iron Status and Ionome in Pea

2020

National audience; Including more grain legumes in cropping systems is important for the development of agroecological practices and the diversification of protein sources for human and animal consumption. Grain legume yield and quality is impacted by abiotic stresses resulting from fluctuating availabilities in essential nutrients such as iron deficiency chlorosis (IDC). Promoting plant iron nutrition could mitigate IDC that currently impedes legume cultivation in calcareous soils, and increase the iron content of legume seeds and its bioavailability. There is growing evidence that plant microbiota contribute to plant iron nutrition and might account for variations in the sensitivity of pe…

0106 biological sciences0301 basic medicineSiderophoresiderophorepeaPlant ScienceBiologylcsh:Plant cultureIron defciency01 natural sciences03 medical and health scienceschemistry.chemical_compoundiron deficiencyPseudomonasplant iron nutritionlcsh:SB1-1110CultivarIron deficiency (plant disorder)LegumeOriginal Research2. Zero hungerRhizosphereChlorosisPyoverdinepyoverdinefood and beverages15. Life on landHorticulture030104 developmental biologychemistry[SDE]Environmental SciencesIDCIonomics010606 plant biology & botanyFrontiers in Plant Science
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Is the ballan wrasse (Labrus bergylta) two species? Genetic analysis reveals within-species divergence associated with plain and spotted morphotype f…

2016

Abstract The ballan wrasse (Labrus bergylta) is a marine fish belonging to the family Labridae characterized by 2 main morphotypes that occur in sympatry: spotty and plain. Previous studies have revealed differences in their life‐history traits, such as growth and maturation; however, the genetic relationship between forms is presently unknown. Using 20 recently developed microsatellite markers, we conducted a genetic analysis of 41 and 48 spotty and plain ballan wrasse collected in Galicia (northwest Spain). The 2 morphotypes displayed highly significant genetic differences to each other (F ST = 0.018, P < 0.0001). A similar degree of genetic differentiation (F ST = 0.025, P < 0.0001) was …

0106 biological sciences0301 basic medicineSympatryballan wrasseShort CommunicationZoologyGenetic relationshipLabrus bergyltaDNA Mitochondrial010603 evolutionary biology01 natural sciencesGenetic analysismicrosatellitesPerciformes03 medical and health sciencesAnimalsbiologyGenetic heterogeneitycolor morphotypesSequence Analysis DNALabrus bergyltabiology.organism_classificationPerciformesSympatry030104 developmental biologyspeciationSpainWrasseMicrosatelliteAnimal Science and ZoologyMicrosatellite RepeatsIntegrative Zoology
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Two consecutive Wolbachia ‐mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae)

2019

Swallowtail butterflies (Papilionidae) are among the most spectacular and well-known Lepidoptera in the European fauna, but their systematics is not fully elucidated. A notable case is that of Iphiclides feisthamelii which, after more than 180 years since description, still has a debated status, being often considered as a subspecies of Iphiclides podalirius. To elucidate the relationship between the two taxa and the evolutionary processes that led to their separation, we combine mitochondrial and nuclear DNA (mtDNA and nDNA) data, Wolbachia screening, genitalia morphology and wing UV reflectance. Our results show that the two taxa clearly differ in male and female genital morphology, male …

0106 biological sciences0301 basic medicineSystematicsSpecies complexEuropean Regional Development FundIntrogressionBiologyGenetic introgression010603 evolutionary biology01 natural sciencesMito‐nuclear discordanceLepidoptera genitalia03 medical and health sciencesSystematicsWolbachia infectionGeneticsMolecular BiologyEcology Evolution Behavior and Systematicsbiology.organism_classificationLepidoptera030104 developmental biologyEvolutionary biologyCryptic speciesAnimal Science and ZoologyWolbachiaTaxonomy (biology)Zoologica Scripta
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Updated pest categorisation of Xylella fastidiosa

2018

Abstract Following a request from the European Commission, the EFSA Plant Health Panel updated its pest categorisation of Xylella fastidiosa, previously delivered as part of the pest risk assessment published in 2015. X. fastidiosa is a Gram‐negative bacterium, responsible for various plant diseases, including Pierce's disease, phony peach disease, citrus variegated chlorosis, olive quick decline syndrome, almond leaf scorch and various other leaf scorch diseases. The pathogen is endemic in the Americas and is present in Iran. In the EU, it is reported in southern Apulia in Italy, on the island of Corsica and in the Provence‐Alpes‐Côte d'Azur region in France, as well as in the Autonomous r…

0106 biological sciences0301 basic medicine[SDV]Life Sciences [q-bio]Plant Science01 natural scienceslaw.inventionplant pestlawolive quick decline syndromePierce's diseaseolive quick.media_common2. Zero hungerpest riskquarantinefood and beverages3. Good healthHorticulturecitrus variegated chlorosis; European Union; leaf scorch; olive quick decline syndrome; pest risk; Pierce's disease; plant health; plant pest; quarantineSettore AGR/12 - PATOLOGIA VEGETALEWoody plantSciences exactes et naturellesPierce’s diseaseVeterinary (miscellaneous)Leaf scorchBiologyleaf scorchMicrobiology03 medical and health sciencesQuarantineOrnamental plantmedicinemedia_common.cataloged_instanceEuropean UnionEuropean unionEuropean Union pest risk plant health plant pest quarantine leaf scorch citrus variegated chlorosis Pierce’s disease olive quick decline syndromeHost (biology)citrus variegated chlorosisfungi15. Life on landbiology.organism_classificationmedicine.disease[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyScientific Opinion030104 developmental biologyAnimal Health and Welfare[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieAnimal Science and ZoologyParasitologyPEST analysisXylella fastidiosaplant health010606 plant biology & botanyFood Science
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mtDNA diversity in rabbit population from Sicily (Italy)

2017

The European rabbit Oryctolagus cuniculus (O.c) lives all over the world and it represents an important resource for many predators. It has been classified as a Near-Threatened species in the Red List of Vertebrates of Italy. It is present in mediterranean basin as two known subspecies: O.c. cuniculus and O.c. algirus. The mediterranean geographic distribution of the two subspecies is still not well known. In particular, in Sicily, lacking of deep studies, is based on the body size and morphological characteristics; there wasn’t a complete description of the actual existing subspecies and previous studies only reported the morphological characteristics of the sicilian rabbit population. In …

0106 biological sciences0301 basic medicineeducation.field_of_studyMitochondrial DNAbiologyEcologymedia_common.quotation_subjectPopulationBiodiversitySettore BIO/05 - ZoologiaBiodiversityrabbit taxonomycyt b mitochondrial DNAOryctolagus cuniculusSubspecies010603 evolutionary biology01 natural sciencesPredation03 medical and health sciences030104 developmental biologybiology.domesticated_animalIUCN Red ListAnimal Science and ZoologyEuropean rabbiteducationBiodiversity Ecosystems conservation Rabbit taxonomy cytb mitochondrial DNA.Diversity (politics)media_common
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Variation in gene expression within clones of the earthworm Dendrobaena octaedra

2017

Gene expression is highly plastic, which can help organisms to both acclimate and adapt to changing environments. Possible variation in gene expression among individuals with the same genotype (among clones) is not widely considered, even though it could impact the results of studies that focus on gene expression phenotypes, for example studies using clonal lines. We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. Five microsatellite markers were developed and used to confirm that offspring are genetic clones of their parent. After that, expression of 12 genes was measured…

0106 biological sciences0301 basic medicinegenotypeClone (cell biology)lcsh:Medicineearthworms01 natural sciencesMicrosatellite LociDendrobaena octaedraGenotypeGene expressiongeeniekspressioAnnelidslcsh:ScienceGeneticsMultidisciplinarykloonitPhenotypePhenotypesMicrosatelliteGene CloningResearch ArticleGenotypingBiologyResearch and Analysis Methods010603 evolutionary biologygenotyyppi03 medical and health sciencesExtraction techniquesclonesGene TypesApomixisGeneticsAnimalsOligochaetaparthenogenesisMolecular Biology TechniquesMolecular BiologyGenepartenogeneesita1184lcsh:ROrganismsBiology and Life SciencesParthenogenesisInvertebratesRNA extraction030104 developmental biologygene expressionta1181lcsh:QMicrosatellite RepeatsCloningPLoS ONE
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Variation in the COI gene of the freshwater pearl mussel Margaritifera margaritifera from River Vuokkijoki

2017

The freshwater pearl mussel Margaritifera margaritifera L. is one of the most endangered freshwater mussels in the world. Effective conservation of threatened species requires not only ecological, but also genetic information from the target species and populations. Since low genetic diversity can reduce the ability of a species to adapt to environmental changes, maintaining genetic diversity has been identified as one of the key elements in successful conservation programs. We examined genetic variation of the freshwater pearl mussel from the River Vuokkijoki, Karelia, Russia. We sequenced a fragment of the cytochrome c oxidase subunit I gene (COI) from 22 individuals and compared the data…

0106 biological sciences0301 basic medicinehaplotypescytochrome c oxidase subunit I gene (COI)PopulationEndangered species010603 evolutionary biology01 natural sciencesGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesfreshwater pearl musselGenetic variationeducationMargaritiferaVuokkijokieducation.field_of_studyGenetic diversitybiologyEcologyCytochrome c oxidase subunit Ifungigenetic diversitybiology.organism_classification030104 developmental biologyMargaritifera margaritiferaFreshwater pearl musselThreatened speciesta1181General Agricultural and Biological Sciences
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Phytotoxic Metabolites Isolated from Neufusicoccum batangarum, the Causal Agent of the Scabby Canker of Cactus Pear (Opuntia ficus-indica L.)

2020

Six phytotoxins were obtained from the culture filtrates of the ascomycete Neofusicoccum batangarum, the causal agent of the scabby canker of cactus pear (Opuntia ficus-indica L.) in minor Sicily islands. The phytotoxins were identified as (&minus

0106 biological sciences<i>neofusicoccum batangarum</i>Health Toxicology and MutagenesisOpuntia ficuslcsh:MedicineBiologyToxicology01 natural sciencesArticlephytotoxinsAscomycotamedicineNuclear Magnetic Resonance BiomolecularPlant DiseasesCankerPEARphytotoxinMolecular Structure010405 organic chemistryHost (biology)lcsh:ROpuntiaNeofusicoccum batangarumMycotoxinsmedicine.diseaseNeofusicoccum batangarum0104 chemical sciencesHorticultureFruitCactuscactus pearPhytotoxicityscabby cankers010606 plant biology & botanyToxins
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