Search results for "perossidasi"

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Effects of ageing on peroxidase activity and localization in radish (Raphanus sativus L.) seeds.

2010

Peroxidase activity was assayed in crude extracts of integument, cotyledons and embryo axis of radish seeds, deteriorated under accelerated ageing conditions. Over five days of ageing, in which germination decreased from 100 to 52%, the enzyme activity in integument was higher than that in other seed parts, increasing in the first days of ageing and then decreasing sharply in extremely aged seeds. Polyacrylamide gel electrophoresis analysis showed four peroxidase isoenzymes with MM of 98, 52.5, 32.8 and 29.5 kDa in the embryo axis of unaged seeds, and only the 32.8 and 29.5 kDa MM isoforms in the integument and cotyledons. In these parts of the seed, only the 29.5 kDa MM isoenzyme increased…

AgingHistologyBiophysicsRaphanusGerminationRaphanusSettore BIO/01 - Botanica Generaleistochimicalcsh:QH301-705.5PeroxidaseinvecchiamentobiologyHistocytochemistryElectric Conductivityfood and beveragesRaphanus sativusEmbryoCell Biologybiology.organism_classificationMolecular biologyEnzyme assayIsoenzymesAgeingBiochemistrylcsh:Biology (General)SpectrophotometryGerminationAgeingSeedsbiology.proteinCytochemistryCytochemistrymorfologia del semeElectrophoresis Polyacrylamide GelIntegumentperossidasianlisi di immaginePeroxidase
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