Search results for "pillar"

showing 10 items of 877 documents

BRAFV600E Mutation and p27kip1 Expression in Papillary Carcinomas of the Thyroid ≤1 cm and Their Paired Lymph Node Metastases

2007

BACKGROUND. BRAFV600E mutation and p27kip1 expression have been introduced as novel indicators that may predict prognosis in different tumors, as well as in papillary thyroid carcinomas. METHODS. Tissue samples from 214 consecutive patients who underwent total or near-total thyroidectomy with histological diagnosis of papillary thyroid carcinoma (PTC) ≤1 cm were analyzed for BRAFV600E mutation by a real-time, allele-specific amplification and for p27kip1 expression by immunohistochemistry. RESULTS. The BRAFV600E mutation was detected in 88 of the tumors examined, with significant differences between groups with and without lymph node (LN) metastases; the mean age of patients with BRAFV600E …

AdultMaleProto-Oncogene Proteins B-rafCancer ResearchPathologymedicine.medical_specialtymedicine.medical_treatmentDown-RegulationGlutamic AcidSettore MED/08 - Anatomia PatologicaPolymerase Chain ReactionMetastasisSettore MED/13 - EndocrinologiaThyroid carcinomaPredictive Value of TestsCarcinomaBiomarkers TumorMedicineHumansThyroid NeoplasmsLymph nodebusiness.industryThyroidThyroidectomyCancerValineSequence Analysis DNAMiddle Agedmedicine.diseasePrognosispapillary thyroid carcinoma BRAF p27 cell cycle.ImmunohistochemistryCarcinoma PapillaryGene Expression Regulation Neoplasticmedicine.anatomical_structureOncologyLymphatic MetastasisMutation (genetic algorithm)MutationFemalebusinessCyclin-Dependent Kinase Inhibitor p27
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Papillary thyroid cancer incidence in the volcanic area of Sicily.

2009

Abstract BACKGROUND: The steadily increasing incidence of thyroid cancer has been attributed mostly to more sensitive thyroid nodule screening. However, various environmental factors, such as those associated with volcanic areas, cannot be excluded as risk factors. We evaluated thyroid cancer incidence in Sicily, which has a homogenous population and a province (Catania) that includes the Mt Etna volcanic area. METHODS: In a register-based epidemiological survey, we collected all incident thyroid cancers in Sicily from January 1, 2002, through December 31, 2004. The age-standardized incidence rate for the world population (ASR(w)) was calculated and expressed as the number of thyroid cancer…

AdultMaleProto-Oncogene Proteins B-rafCancer Researchmedicine.medical_specialtyPathologyAdolescentPopulationPrevalenceVolcanic EruptionsThyroid CancerRisk AssessmentSettore MED/13 - EndocrinologiaPapillary thyroid cancerThyroid cancer environmental factors SicilyYoung AdultAge DistributionVolcanoes and Thyroid CancerRisk FactorsEpidemiologyAdenocarcinoma FollicularmedicineHumansEnvironment and Thyroid CancerRegistriesThyroid NeoplasmsSex DistributioneducationThyroid cancerSicilyAgedAged 80 and overeducation.field_of_studyObstetricsbusiness.industryIncidence (epidemiology)IncidenceThyroidCancerEnvironmental ExposureMiddle Agedmedicine.diseaseCarcinoma Papillarymedicine.anatomical_structureOncologyCarcinoma MedullaryMutationFemalebusinessJournal of the National Cancer Institute
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Fine-Needle Aspiration (FNAB) Molecular Analysis for the Diagnosis of Papillary Thyroid Carcinoma through BRAFv600E mutation and RET/PTC rearrangement

2007

Objective: To evaluate BRAFV600E mutation on consecutive fine-needle aspiration biopsy (FNAB) specimens in order to assess FNAB’s usefulness in preoperative papillary thyroid carcinoma (PTC) diagnosis with the contemporaneous analysis of RET=PTC1 and RET=PTC3 rearrangements obtained from ex vivo thyroid nodules. Design: Thyroid FNABs from 156 subjects with nodules and 49 corresponding surgical samples were examined for the presence of BRAF mutation by real-time allele-specific polymerase chain reaction, confirmed with the use of a laser pressure catapulting system. Samples were also examined for RET=PTC rearrangements. The results were compared with the cytological diagnosis and histopathol…

AdultMaleProto-Oncogene Proteins B-rafPathologymedicine.medical_specialtyendocrine system diseasesEndocrinology Diabetes and MetabolismBiopsyBiopsy Fine-NeedlePapillarySettore MED/08 - Anatomia PatologicaRET/PTCSettore MED/13 - EndocrinologiaThyroid carcinomaCohort StudiesEndocrinologyPredictive Value of TestsBiopsyCarcinomamedicine80 and overHumansThyroid NeoplasmsAgedRET/PTC RearrangementAged 80 and overGene Rearrangementmedicine.diagnostic_testbusiness.industryCarcinomaProto-Oncogene Proteins c-retGene rearrangementMiddle Agedmedicine.diseaseCarcinoma PapillaryAdult; Aged; 80 and over; Amino Acid Substitution; Biopsy; Fine-Needle; Carcinoma; Papillary; Cohort Studies; Female; Gene Rearrangement; Humans; Male; Middle Aged; Predictive Value of Tests; Proto-Oncogene Proteins B-raf; Proto-Oncogene Proteins c-ret; Thyroid NeoplasmsBrafBRAF V600EFine-needle aspirationAmino Acid SubstitutionMutation (genetic algorithm)Fine-NeedleFemalebusiness
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Effect of intraarterial papaverine or nimodipine on vessel diameter in patients with cerebral vasospasm after subarachnoid hemorrhage.

2012

Papaverine (P) and nimodipine (N) are the most widely used vasodilators when angiographic and symptomatic vasospasm is present after subarachnoid aneurysmatic hemorrhage (SAH). Their effect is only short-lived and no direct comparisons have been undertaken to evaluate the action of both substances directly. We retrospectively assessed the effect of either P or N on angiographic diameter reduction and capillary blood flow.Fifteen SAH patients with secured aneurysms and cerebral vasospasm received intraarterial P, fifteen similar patients received N. As the primary endpoint, pre- and post-infusion arterial diameters and capillary blood flow were rated retrospectively on angiographies and comp…

AdultMaleSubarachnoid hemorrhageVasodilator AgentsIschemiaBrain IschemiaCerebral vasospasmModified Rankin ScalePapaverinemedicineHumansInfusions Intra-ArterialVasospasm Intracranialcardiovascular diseasesNimodipineAgedRetrospective StudiesAged 80 and overPapaverineAnalysis of Variancebusiness.industryVasospasmGeneral MedicineBlood flowCerebral ArteriesMiddle AgedSubarachnoid Hemorrhagemedicine.diseasenervous system diseasesCapillariesVasodilationAnesthesiaCerebrovascular CirculationSurgeryFemaleNimodipineNeurology (clinical)businessCarotid Artery Internalmedicine.drugBritish journal of neurosurgery
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Periodontal disease and sjogren syndrome: a possible correlation?

2009

Sjögren syndrome (SS) is a chronic autoimmune rheumatic disease characterized by a progressive lymphocytic infiltration of exocrine glands, especially salivary and lachrymal ones, leading to xerostomia, parotid gland enlargement, and xerophthalmia. The aim of this study is to describe the capillaroscopic pattern of the interdental papilla in patients with SS and to evaluate a possible correlation with periodontal disease. Methods: A total of 25 patients affected by SS and 25 healthy controls were examined. The patients with conditions that compromise microcirculation, such as diabetes, hypertension, hyperlipidemia, or some pharmacological treatments, were not included in the study. All the…

AdultMaleSystemic diseasePathologymedicine.medical_specialtyEye diseaseGingivaMicrocirculationperiodontal disease sjogren syndromMicroscopic AngioscopySettore MED/28 - Malattie OdontostomatologicheDiabetes mellitusImmunopathologymedicineHumansXerophthalmiaInterdental papillaPeriodontal DiseasesAgedAutoimmune diseasebusiness.industryMicrocirculationMiddle Agedmedicine.diseaseCapillariesmedicine.anatomical_structureSjogren's SyndromeFemaleCardiology and Cardiovascular MedicinebusinessAngiology
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Diabetic Microagiopathy of Oral Mucosa Depends on Disease Duration and Therapy

2017

Background: Diabetes mellitus is a chronic degenerative systemic disease whose prevalence is increasing. This paper aims to evaluate the effects of diabetic microangiopathy, depending on its duration and the type of treatment administered, by using polarized light videocapillaroscopy of the oral mucosa. Material/Methods: We enrolled 120 subjects: 60 healthy subjects and 60 patients with diabetes mellitus. In turn, patients were divided into 3 subgroups according to the type of diabetes, the duration of the disease, and the type of treatment administered. A videocapillaroscopic examination of the oral mucosa was carried out on the diabetic and healthy subjects. Results: Changes in microcircu…

AdultMaleSystemic diseasemedicine.medical_specialtymedicine.medical_treatment030209 endocrinology & metabolismGastroenterologyMicroscopic AngioscopyMicrocirculationDiabetes Complications03 medical and health sciences0302 clinical medicineTongueClinical ResearchTongueDiabetes mellitusInternal medicinemedicineHumansOral mucosaType 1 diabetesbusiness.industryMicrocirculationInsulinMouth Mucosa030206 dentistryGeneral MedicineBuccal administrationMiddle Agedmedicine.diseaseCapillariesDiabetes Mellitus Type 1medicine.anatomical_structureDiabetes Mellitus Type 2Dentistry Diabetes Complications Microcirculation Microscopic AngioscopyDentistryFemalebusinessDiabetic AngiopathiesMedical Science Monitor
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Longitudinal analysis of taurine induced effects on the tear proteome of contact lens wearers and dry eye patients using a RP-RP-Capillary-HPLC-MALDI…

2012

Tear proteomic studies revealed distinct similarities between contact lens wearers and dry eye patients. AMO Complete® multipurpose contact lens cleaning solutions containing taurine seem to have a beneficial effect regarding contact lens induced dry eye. To illuminate the effect of taurine on the tear proteome of contact lens wearers and sicca patients we developed a gel-based RP-RP capillary HPLC-MALDI TOF/TOF MS strategy. Two contact lens wearer groups, one using eye drops containing 0.05% taurine; the other for control physiological NaCl solution were monitored. Also, a third group of sicca patients using taurine solution was studied (N=4 individuals/group). Tear pools of each group at …

AdultMaleTaurinemedicine.medical_specialtyTime FactorsProteomeCapillary actionContact LensesTaurineBiophysicsAnalytical chemistryBiologyBiochemistryHigh-performance liquid chromatographychemistry.chemical_compoundOphthalmologymedicineHumansLongitudinal StudiesChromatography High Pressure Liquideye diseasesContact lensMatrix-assisted laser desorption/ionizationmedicine.anatomical_structurechemistryLens (anatomy)Spectrometry Mass Matrix-Assisted Laser Desorption-IonizationTearsProteomeTearsDry Eye SyndromesFemaleContact Lens SolutionsJournal of proteomics
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Novel rearrangements involving the RET gene in papillary thyroid carcinoma.

2018

Abstract Background In the field of gene fusions driving tumorigenesis in papillary thyroid carcinoma (PTC), rearrangement of the proto-oncogene RET is the most frequent alteration. Apart from the most common rearrangement of RET to CCDC6, more than 15 partner genes are yet reported. The landscape of RET rearrangements in PTC (“RET-PTC”) can notably be enlarged by modern targeted next-generation sequencing, indicating similarities between oncogenic pathways in other cancer types with identical genetic alterations. Methods Targeted next-generation sequencing was performed for two cases of BRAF-wild type PTC with confirmation of the results by Sanger sequencing. A “UniProt” database research …

AdultMalecongenital hereditary and neonatal diseases and abnormalitiesendocrine systemCancer Researchendocrine system diseasesOncogene Proteins FusionBiologyRUN domainmedicine.disease_causeProto-Oncogene MasFusion gene03 medical and health sciencessymbols.namesake0302 clinical medicineGeneticsmedicineHumansThyroid NeoplasmsneoplasmsMolecular BiologyGeneSanger sequencingGene RearrangementProto-Oncogene Proteins c-retIntracellular Signaling Peptides and ProteinsCancerHigh-Throughput Nucleotide SequencingNuclear ProteinsProtein-Tyrosine Kinasesmedicine.diseaseLisH domainThyroid Cancer Papillary030220 oncology & carcinogenesisCancer researchsymbolsFemaleCarcinogenesisCarrier ProteinsTyrosine kinaseCancer genetics
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Hashimoto Thyroiditis Coexistent with Papillary Thyroid Carcinoma

2005

Several studies report a higher rate of papillary thyroid carcinomas (PTC) in patients with Hashimoto thyroiditis (HT), indicating a possible correlation between the two diseases. We studied a group of 89 subjects undergoing surgery for thyroid carcinomas compared with a control group of 89 subjects operated on for normofunctioning goiter, and a second group of 47 patients undergoing total thyroidectomy for HT. Association with HT was found in 19 of the 71 PTC subjects (26.7%) and in 8 goiter patients (8.9%), which was a significant difference ( P < 0.02). Thirteen of the HT patients, mostly with the nodular form, showed coexistent PTC (27.6%). HT and PTC coexisted in several morphologi…

AdultMaleendocrine systemmedicine.medical_specialtyPathologyGoiterthyroid tumorendocrine system diseasesretrospective studymedicine.medical_treatmentHashimoto Diseasethyroidectomy MeSH: Adenocarcinoma PapillaryGastroenterologyThyroid carcinomaRetrospective StudieInternal medicinemedicineHumanshumanThyroid NeoplasmsThyroid cancerThyroid NeoplasmAgedRetrospective StudiesAutoimmune diseaseadenocarcinoma; adult; aged; article; female; Hashimoto disease; human; male; middle aged; retrospective study; thyroid tumor; thyroidectomy Adenocarcinoma Papillary; Adult; Aged; Female; Hashimoto Disease; Humans; Male; Middle Aged; Retrospective Studies; Thyroid Neoplasms; Thyroidectomy [EMTREE medical terms]business.industryarticleThyroidectomyGeneral MedicineMiddle Agedmedicine.diseaseMajor duodenal papillaAdenocarcinoma PapillaryThyroidectomyImmunohistochemistryAdenocarcinomaFemalebusinessEMTREE medical terms: adenocarcinomaThe American Surgeon
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Epigenetic differences arise during the lifetime of monozygotic twins.

2005

Monozygous twins share a common genotype. However, most monozygotic twin pairs are not identical; several types of phenotypic discordance may be observed, such as differences in susceptibilities to disease and a wide range of anthropomorphic features. There are several possible explanations for these observations, but one is the existence of epigenetic differences. To address this issue, we examined the global and locus-specific differences in DNA methylation and histone acetylation of a large cohort of monozygotic twins. We found that, although twins are epigenetically indistinguishable during the early years of life, older monozygous twins exhibited remarkable differences in their overall…

AdultMalemedicine.medical_specialtyADNRestriction MappingTwinsMonozygotic twinX-inactivationEpigenesis GeneticHistonesX Chromosome InactivationSurveys and QuestionnairesGenotypemedicineHumansEpigeneticsOligonucleotide Array Sequence AnalysisGeneticsAnalysis of VarianceMultidisciplinarybiologyReverse Transcriptase Polymerase Chain ReactionElectrophoresis CapillaryGene Expression Regulation DevelopmentalAcetylationNucleic acid amplification techniqueDNASequence Analysis DNATwins MonozygoticDNA MethylationExpressió gènicaFenotipHistonePhenotypeSpainDNA methylationbiology.protein5-MethylcytosineCommentaryMedical geneticsBessonsFemaleGene expressionNucleic Acid Amplification TechniquesProceedings of the National Academy of Sciences of the United States of America
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