Search results for "pisum sativum"
showing 3 items of 43 documents
Generation of homozygosity and genome fixation in pea (Pisum sativum L.)
2013
Pea cultivars are nearly homozygous and thus homogeneous when they are released. The traditional method of selfing is slow and inefficient, taking up to ten generations of inbreeding following a cross to achieve a high level of homozygosity. Current single-seed-descent (SSD) methodologies enable a maximum of three generations per year to be developed in pea. Doubled haploidy and an in vitro based modified SSD technology have been utilised in many important crops for the rapid achievement of homozygosity, and thus acceleration of the breeding process. In pea, due to the lack of robust protocols, none of these technologies is routinely used in a breeding program. The aim of this study was to …
The management of crop-livestock systems at a territory level: the example of grain legume crops and of monogastric livestock in Burgundy, France
2012
Although cereals and oil seed crops are very developed in Burgundy, the area of grain legumes cultivation is small. Concerning monogastric livestock, the region is characterised by a medium size poultry production focussed on markets under quality and origin signs (SIQO) and a relatively small pig production. Although a tendency of co-location of these productions can be observed (at territory and farm scales) and some animals consume locally-produced proteins, there is no strong functional link between these crops and animal activities. This situation is confirmed by the fact that a significant proportion of produced grain legumes are exported by Burgundy, while large quantities of soybean…
Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile
2004
Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments…