Search results for "plasmalemma"
showing 2 items of 2 documents
Atomic-force microscopy imaging of plasma membranes purified from spinach leaves
2000
Summary: Plasma membranes purified from spinach leaves by aqueous two-phase partitioning were examined by atomic-force microscopy (AFM) in phosphate buffer, and details on their structure were reported at nanometric scale. Examination of the fresh membrane preparation deposited on mica revealed a complex organization of the surface. It appeared composed of a first layer of material, about 8 nm in thickness, that practically covered all the mica surface and on which stand structures highly heterogeneous in shape and size. High-resolution imaging showed that the surface of the first layer appeared relatively smooth in some regions, whereas different characteristic features were observed in ot…
Characterization of membrane structure modifications induced during plant defense signaling
2017
SPEIPMUBINRADOCT; Plasma membrane was long considered as a simple separation between internal and external cellular environment. But more, it is a dynamic interface, an exchange platform. Now, recent researches highlight its role in plant defense responses to biotic and abiotic stresses. We use the tobaccocryptogein model to study the early steps of plant cell responses to pathogen detection. The signaling cascade includes an oxidative burst triggering an increase of plasma membrane order. The level of membrane order informs us about both packing and arrangement of membrane components, this parameter being evaluated by spectrofluorimetric approaches using a lipidic environment-sensitive pro…