Search results for "play"

A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors

2021

Delivering genes selectively to the therapeutically relevant cell type is among the prime goals of vector development. Here, we present a high-throughput selection and screening process that identifies designed ankyrin repeat proteins (DARPins) optimally suited for receptor-targeted gene delivery using adeno-associated viral (AAV) and lentiviral (LV) vectors. In particular, the process includes expression, purification, and in situ biotinylation of the extracellular domains of target receptors as Fc fusion proteins in mammalian cells and the selection of high-affinity binders by ribosome display from DARPin libraries each covering more than 1012 variants. This way, DARPins specific for the …

2018

Background Attempts to establish a biomarker reflecting individual player load in intermittent sports such as football have failed so far. Increases in circulating DNA (cfDNA) have been demonstrated in various endurance sports settings. While it has been proposed that cfDNA could be a suitable marker for player load in intermittent sports, the effects on cfDNA of repeated sprinting as an essential feature in intermittent sports are unknown. For the first time, we assessed both alterations of cfDNA due to repeated maximal sprints and due to a professional football game. Methods Nine participants were subjected to a standardised sprint training session with cross-over design of five maximal s…

Symptomatic accommodative and binocular dysfunctions from the use of flat-panel displays

2018

AIM: To determine the presence of symptomatic accommodative and non-strabismic binocular dysfunctions (ANSBD) in a non-presbyopic population of video display unit (VDU) users with flat-panel displays. METHODS: One hundred and one VDU users, aged between 20 to 34y, initially participated in the study. This study excluded contact-lens wearers and subjects who had undergone refractive surgery or had any systemic or ocular disease. First, subjects were asked about the type and nature of eye symptoms they experienced during VDU use. Then, a thorough eye examination excluded those subjects with a significant uncorrected refractive error or other problem, such as ocular motility disorders, vertica…

Getting into the Same Boat – Enabling the Realization of the Disabled Child’s Agency in Adult–Child Play Interaction

2021

The purpose of this study was to find out how an adult can enable or hinder the realization of a disabled child’s agency in play interaction. We focused on the child’s play invitations, which were constructed as dispreferred by the adult. The data consisted of nine videotaped playing situations with five nurses and five disabled children in a children’s neurological ward. The microanalysis with interventionist applied conversation analysis focused on one playing situation between one nurse and one three-year-old boy with no spoken language. The nurse responded to the child’s play invitations constructed as dispreferred by her in three different ways. Two of them were about trying to control…

The effects of playing tactics on creating scoring opportunities in random matches from US Major League Soccer

2015

The aim of this study was to examine the effects of playing tactics on the creation of scoring opportunities in random matches from US Major League Soccer 2014, taking into account both contextual and defensive variables. The sample included 3253 team possessions corresponding to 30 randomly selected matches. Multidimensional qualitative data from 10 categorical variables was analysed by means of descriptive analysis and multiple logistic regression analysis. Contextual variables such as match location (p=0.049), match half (p=0.043) and match status (P=0.032) were associated with creating scoring opportunities. With regards playing tactics, set plays created a higher proportion of scoring …

Body coloration and mechanisms of colour production in Archelosauria: the case of deirocheline turtles

2019

Animal body coloration is a complex trait resulting from the interplay of multiple mechanisms. While many studies address the functions of animal coloration, the mechanisms of colour production still remain unknown in most taxa. Here we compare reflectance spectra, cellular, ultra- and nano-structure of colour-producing elements, and pigment types in two freshwater turtles with contrasting courtship behaviour,Trachemys scriptaandPseudemys concinna. The two species differ in the distribution of pigment cell-types and in pigment diversity. We found xanthophores, melanocytes, abundant iridophores and dermal collagen fibres in stripes of both species. The yellow chin and forelimb stripes of bot…

La Sala Parpalló en el Centre Cultural La Beneficència (1995-1999)

2020

During the period 1995-1999, the Sala Parpalló was located in the Beneficència Cultural Center of Valencia, sharing the place with the spaces of the Museum of Prehistory, the Museum of Ethnology and the IVEI. In the investigation it exposed a technical file for each of the 57 self-produced exhibitions carried out in the period and a selection of the studies that accompanied them: the files are displayed chronologically according to the procedures used. The work concludes with the analysis of the objectives that were mainly all the programming: 1) Sala Parpalló as an integrated project; 2) Studies and exhibitions around the Valencian Cultural Imaginary; 3) Analysis of the postmodern crisis; …

Safety Protocols for Forensic Inspections in the Time of COVID-19: An Approach to Protect Practitioners

2022

The COVID-19 pandemic has impacted the entire world in ways that were unimaginable in 2019. It has changed how professionals work and interact with one another. To ensure the safety of experts and attorneys, forensic inspection procedures must evolve while still providing a reliable foundation for expert testimony. Traditional field inspections require attendees to be physically present and in generally close proximity. Inspecting artifacts may also require that individual components be serially examined by multiple experts, which increases the risk of transmitting the disease. The authors propose a new approach to forensic inspections aimed at protecting consultants from the biological ris…

Enhanced field-of-view integral imaging display using multi-Köhler illumination.

2014

A common drawback in 3D integral imaging displays is the appearance of pseudoimages beyond the viewing angle. These pseudoimages appear when the light rays coming from each elemental image are not passing through the corresponding microlens, and a set of barriers must be used to avoid this flipping effect. We present a pure optical arrangement based on Köhler illumination to generate these barriers thus avoiding the pseudoimages. The proposed system does not use additional lenses to project the elemental images, so no optical aberrations are introduced. As an added benefit, Köhler illumination provides a higher contrast 3D display. This work was supported in part by the Plan Nacional I + D …

Retinoic-Acid-Induced Downregulation of the 67 KDa Laminin Receptor Correlates with Reduced Biological Aggressiveness of Human Neuroblastoma Cells

2012

Neuroblastoma is a common tumor of the childhood arising from embryonal sympathetic neural cell precursors. Despite of the improved therapeutic strategies, the survival rate of high-risk neuroblastoma patients is poor. Although complete clinical remissions can be achieved, relapse is relatively frequent, indicating a role for the persistence of the minimal residual disease (for review, Maris, 2010). Treatments with derivatives of retinoic acid (RA), the biologically active form of vitamin A, produce significant improvements on the therapy of high-risk neuroblastoma patients, when used together with intensive multimodal therapies (Reynolds et al., 2003, for review). Despite some controversy …