Search results for "pluripotent"
showing 6 items of 96 documents
Pluripotent stem cells to model Hutchinson-Gilford progeria syndrome (HGPS): Current trends and future perspectives for drug discovery
2015
Progeria, or Hutchinson-Gilford progeria syndrome (HGPS), is a rare, fatal genetic disease characterized by an appearance of accelerated aging in children. This syndrome is typically caused by mutations in codon 608 (p.G608G) of the LMNA, leading to the production of a mutated form of lamin A precursor called progerin. In HGPS, progerin accumulates in cells causing progressive molecular defects, including nuclear shape abnormalities, chromatin disorganization, damage to DNA and delays in cell proliferation. Here we report how, over the past five years, pluripotent stem cells have provided new insights into the study of HGPS and opened new original therapeutic perspectives to treat the disea…
Modelos in vitro personalizados basados en células pluripotentes para el estudio de la toxicidad hepática inducida por fármacos de carácter idiosincr…
2023
El daño hepático inducido por fármacos (DILI) es una reacción adversa a medicamentos y otros xenobióticos que representa una de las causas más relevantes de fallo hepático agudo y es uno de los principales motivos de retirada de fármacos en las fases de desarrollo y farmacovigilancia. El DILI es un proceso patológico multifactorial que no se puede recapitular en los modelos in vitro actuales. Además, el DILI idiosincrático (iDILI), que es aquel que aparece en un reducido número de pacientes susceptibles, es difícil de diagnosticar y predecir en los modelos propuestos hasta el momento. Por esta razón, se han propuesto nuevos modelos capaces de reproducir las características específicas de pa…
Management of liver failure: from transplantation to cell-based therapy
2011
The severe shortage of deceased donor organs has driven a search for alternative methods of treating liver failure. In this context, cell-based regenerative medicine is emerging as a promising interdisciplinary field of tissue repair and restoration, able to contribute to improving health in a minimally invasive fashion. Several cell types have allowed long-term survival in experimental models of liver injury, but their therapeutic potential in humans should be regarded with deep caution, because few clinical trials are currently available and the number of patients enrolled so far is too small to assess benefits versus risks. This review summarizes the current literature on the physiologi…
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold
2022
Human pluripotent stem cells (hPSC) derived neurons are emerging as a powerful tool for studying neurobiology, disease pathology, and modeling. Due to the lack of platforms available for housing and growing hPSC-derived neurons, a pressing need exists to tailor a brain-mimetic 3D scaffold that recapitulates tissue composition and favourably regulates neuronal network formation. Despite the progress in engineering biomimetic scaffolds, an ideal brain-mimetic scaffold is still elusive. We bioengineered a physiologically relevant 3D scaffold by integrating brain-like extracellular matrix (ECM) components and chemical cues. Culturing hPSCs-neurons in hyaluronic acid (HA) gels and HA-chondroitin…
3AB-OS, a new human pluripotent cancer stem cell: properties and expectations for practical applications.
2009
Reprogramación celular, dinámica mitocondrial y metabolismo
2016
La reprogramación celular es el proceso de reconversión del destino celular somático a un estado pluripotente similar al de las células madre embrionarias. Dadas las importantes diferencias entre ambos estados, esta transformación supone una profunda reorganización del fenotipo celular a todos los niveles. Para ello, las células somáticas deben sortear una serie de barreras celulares y moleculares a lo largo de este proceso hasta alcanzar su destino final de célula madre pluripotente inducida. Entre las importantes diferencias que presentan ambos tipos celulares resaltan la morfología mitocondrial y el metabolismo celular: durante la reprogramación celular las células somáticas tienen que m…