Search results for "polyaspartammide"

showing 2 items of 2 documents

Reversibly stable thiopolyplexes for intracellular delivery of genes.

2006

Novel polyaspartamide non-viral carriers for gene therapy were synthesized by introducing, on the same polymer backbone, positively charged groups, for electrostatic interactions with DNA, and thiol groups for the formation of disulfide bridges between polymer chains. The introduction of thiols was aimed to have a vector with low redox potential sensitivity: disulfide crosslinking in fact, being stable in extracellular environment, allowed either to have stable complexes in plasma, that can protect DNA from metabolism, or to be reduced inside the cell, where the excess of glutathion in reduced form maintains a low redox potential. The consequent destabilization of the complex after disulfid…

Magnetic Resonance SpectroscopyLightStereochemistryCell SurvivalPolymersPharmaceutical ScienceElectrophoretic Mobility Shift AssayGene deliveryTransfectionchemistry.chemical_compoundGene DeliveryMiceDynamic light scatteringGenes ReporterCell Line TumorAnimalsScattering RadiationElectrophoretic mobility shift assayDisulfidesSulfhydryl CompoundsLuciferaseschemistry.chemical_classificationthiopolycationsEndodeoxyribonucleasesLuminescent AgentsGenetic transferCationic polymerizationProteinsDNAChromatography Ion ExchangeCombinatorial chemistrychemistrypolyaspartammideAgarose gel electrophoresisThiolPeptidesOxidation-ReductionDNAJournal of controlled release : official journal of the Controlled Release Society
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Tamoxifen-loaded polymeric micelles: preparation, physico-chemical characterization and in vitro evaluation studies.

2004

Several samples of polymeric micelles, formed by amphiphilic derivatives of PHEA, obtained by grafting into polymeric backbone of PEGs and/or hexadecylamine groups (PHEA-PEG-C(16) and PHEA-C(16)) and containing different amount of Tamoxifen, were prepared. All Tamoxifen-loaded polymeric micelles showed to increase drug water solubility. TEM studies provided evidence of the formation of supramolecular core/shell architectures containing drug, in the nanoscopic range and with spherical shape. Samples with different amount of encapsulated Tamoxifen were subjected to in vitro cytotoxic studies in order to evaluate the effect of Tamoxifen micellization on cell growth inhibition. All samples of T…

Polymers and PlasticsAntineoplastic Agents HormonalPolymersSupramolecular chemistryBioengineeringMicellePolyethylene GlycolsBiomaterialsPlasmaDrug Delivery SystemsTamoxifen polymeric micelles polyaspartammideAmphiphileMaterials ChemistryOrganic chemistryHumansMicellesAqueous solutionMolecular StructureChemistryHydrogen-Ion ConcentrationTamoxifenMembraneSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDrug deliveryLiberationDrug carrierPeptidesBiotechnologyNuclear chemistryMacromolecular bioscience
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