Search results for "porat"

A novel plasmid DNA electroporation method allows transfection of murine DC.

2007

Under steady state conditions dendritic cells (DC) exert tolerogenic function, but acquire potent immunogenic function due to strong upregulation of costimulatory molecules and proinflammatory cytokines. In numerous studies the potential of modified DC to induce tolerance or immune reactions towards a distinct antigen has been demonstrated. However, DC are refractory to transfection with plasmid DNA by non-viral methods. In this study we have tested the suitability of a newly developed electroporation device to transfect immature murine bone-marrow derived DC (BM-DC). Transfected BM-DC expressed reporter molecules at considerable extent which renders this method suitable to perform all kind…

CEO’s Letter Accompanying the Report on Corporate social Responsibility of Canadian Listed Firms: An Analysis of the Content and the Links with Some …

2016

International audience; The disclosure of Corporate Social Responsibility (CSR) by Canadian firms is not mandatory. This provides an ideal context to describe the nature of what is being freely disclosed by large firms and to study the firms’ characteristics which lead to such disclosure. We searched all TSX listed firms to find those which disclose a CSR report accompanied by a letter from the CEO. In the end, we study 89 CEO’s letters; that is, all the firms disclosing both a CSR report and a CEO’s letter. We first looked at the nature of the elements disclosed. We classified them in 3 categories: (1) actual accomplishments in the field of CSR, (2) values and (3) future objectives. The «a…

The effect of CEOs’ turnover on the CSP of French firms

2016

International audience

Corporate Resilience in face of the Covid 19: A proposition for a business resilience Index

2021

Titre du congrès : «The Future of Europe Beyond Brexit and COVID-19»; International audience

Legislative basis for corporate social responsibility reporting

2014

CD83+ human dendritic cells transfected with tumor peptide cDNA by electroporation induce specific T-cell responses: A potential tool for gene immuno…

2000

Dendritic cells (DC) are the most potent immunostimulatory cells, with the capacity to induce primary T-cell responses. Functional autologous DC can be generated from fetal calf serum-free peripheral blood mononuclear cells in the presence of interleukin-4 and granulocyte-macrophage colony-stimulating factor and are stimulated with a defined cytokine cocktail for terminal maturation. We were able to establish a nonviral transfection protocol for these DC by electroporation. Using enhanced green fluorescent protein as a reporter gene, we achieved transfection efficiencies of up to 10%. FACScan analyses revealed a stable phenotype, and the expression of major histocompatibility complex class …

Micrometeorological and sap flow measurement of water vapour exchanges in olive: Scaling up from canopy to orchard

2009

A comparison of water consumption evaluated at tree and orchard level was carried out in a commercial olive orchard located in Sicily using up-scaled sap-flow evapotranspiration estimations and eddy covariance measurements. Sap flow probes were installed on olive trees placed in one of the four plots characterizing a heterogeneous orchard. Trees were chosen, from a preliminary footprint analysis, in correspondence to the peak of the “relative normalized contribution” to flux for the prevailing wind conditions measured by an eddy covariance station localized in the central part of the orchard. Tree-age and planting density as well as main tree and orchard characteristics (Leaf Area per tree,…

Simplified Interception/Evaporation Model

2021

It is known that at the event scale, evaporation losses of rainfall intercepted by canopy are a few millimeters, which is often not much in comparison to other stocks in the water balance. Nevertheless, at yearly scale, the number of times that the canopy is filled by rainfall and then depleted can be so large that the interception flux may become an important fraction of rainfall. Many accurate interception models and models that describe evaporation by wet canopy have been proposed. However, they often require parameters that are difficult to obtain, especially for large-scale applications. In this paper, a simplified interception/evaporation model is proposed, which considers a modified …

Building a model of corporate social responsibility in the old industrial region(in the case of Upper Silesia): a sociological perspective

2009

PurposeCorporate social responsibility (CSR) is one of the most crucial phenomena of global capitalism at the beginning of the twenty‐first century. For this reason it is emphasized by many companies (especially transnational corporations and multinational companies) and in the European Union and its policy. The main purpose of this paper is to analyze how CSR exists in a transitional country and region – the Upper Silesian Industrial District and the Rybnik Coalmine Area in Southern Poland.Design/methodology/approachA qualitative and quantitative methodology was used to summarize the sociological research among entrepreneurs and businesses located throughout the region.FindingsThis researc…

Knockout of thep-Coumarate Decarboxylase Gene fromLactobacillus plantarumReveals the Existence of Two Other Inducible Enzymatic Activities Involved i…

2000

ABSTRACTLactobacillus plantarumNC8 contains apdcgene coding forp-coumaric acid decarboxylase activity (PDC). A food grade mutant, designated LPD1, in which the chromosomalpdcgene was replaced with the deletedpdcgene copy, was obtained by a two-step homologous recombination process using an unstable replicative vector. The LPD1 mutant strain remained able to weakly metabolizep-coumaric and ferulic acids into vinyl derivatives or into substituted phenyl propionic acids. We have shown thatL. plantarumhas a second acid phenol decarboxylase enzyme, better induced with ferulic acid than withp-coumaric acid, which also displays inducible acid phenol reductase activity that is mostly active when gl…