Search results for "porte"
showing 10 items of 1894 documents
Response to long-term NaHCO3-derived alkalinity in model Lotus japonicus Ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physi…
2014
The current knowledge regarding transcriptomic changes induced by alkalinity on plants is scarce and limited to studieswhere plants were subjected to the alkaline salt for periods not longer than 48 h, so there is no information availableregarding the regulation of genes involved in the generation of a new homeostatic cellular condition after long-termalkaline stress.Lotus japonicusis a model legume broadly used to study many important physiological processes includingbiotic interactions and biotic and abiotic stresses. In the present study, we characterized phenotipically the response toalkaline stress of the most widely usedL. japonicusecotypes, Gifu B-129 and MG-20, and analyzed global t…
Endogenous fluctuations of DNA topology in the chloroplast of Chlamydomonas reinhardtii.
1998
DNA supercoiling in the chloroplast of the unicellular green alga Chlamydomonas reinhardtii was found to change with a diurnal rhythm in cells growing in alternating 12-h dark-12-h light periods. Highest and lowest DNA superhelicities occurred at the beginning and towards the end of the 12-h light periods, respectively. The fluctuations in DNA supercoiling occurred concurrently and in the same direction in two separate parts of the chloroplast genome, one containing the genes psaB, rbcL, and atpA and the other containing the atpB gene. Fluctuations were not confined to transcribed DNA regions, indicating simultaneous changes in DNA conformation all over the chloroplast genome. Because the d…
The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
2008
Inner nuclear membrane proteins containing a LEM (LAP2, emerin, and MAN1) domain participate in different processes, including chromatin organization, gene expression, and nuclear envelope biogenesis. In this study, we identify a robust genetic interaction between transcription export (TREX) factors and yeast Src1, an integral inner nuclear membrane protein that is homologous to vertebrate LEM2. DNA macroarray analysis revealed that the expression of the phosphate-regulated genes PHO11, PHO12, and PHO84 is up-regulated in src1Δ cells. Notably, these PHO genes are located in subtelomeric regions of chromatin and exhibit a perinuclear location in vivo. Src1 spans the nuclear membrane twice an…
Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography
2005
The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma…
Supported liquid membrane extraction of aromatic aminophosphonates
2001
Abstract A fast and efficient supported liquid membrane (SLM) extraction system for the series of substituted aromatic aminophosphonates was elaborated. The mass transfer of solute was examined in terms of operator (pH and ionic strength of water phase, composition of membrane phase and the concentration of analyte) dependent parameters. Additionally, the influence of the aminophosphonates structure on the extraction efficiency was examined. From the results it can be concluded that the most effective conditions of membrane process are 10% (w/w) carrier concentration (Aliquat 336) in the liquid membrane, high pH of donor phase (pH=11) and moderate salt concentration (0.4 M NaCl) in the acce…
Glucose oxidase as a biocatalytic enzyme-based bio-fuel cell using Nafion membrane limiting crossover
2013
A novel combination for an Enzyme-based Biofuel cell included a Nafion membrane as an ion transporter that maintained a working cell charge and inhibited membrane degradation. The prototype cell chamber used oxygen (O2) in the cathode cell and glucose in the anode. The Nafion membrane stability studied here was evidently in the region of 0% loss of conductivity as the charge was constant and increased after the addition of glucose. The prototype cell chamber used NaCl in the cathode cell and glucose oxidase (GOx) in the anodic chamber was successfully studied for membrane stability showed in this study no evidence of poisoning from membrane leakage in a controlled pH environment. There was …
Un europeísta en acción
2008
Situación actual del personal técnico-deportivo en las empresas del sector en la Comunidad Valenciana
2011
Actualmente, el tema de los recursos humanos está produciendo un fuerte impacto en España y sólo hay que acercarse a la bibliografía empresarial para comprobar que el estudio del sector de la actividad física y el deporte representa un campo de interés científico y social que requiere ser investigado. Mediante una metodología cuantitativa de corte descriptivo y mediante el empleo de la entrevista estandarizada como instrumento de recogida de información se reproducen aquellas características más relevantes observadas en la Comunidad Valenciana. Los resultados explican que las personas que trabajan en funciones físico-deportivas en empresas del sector son mayoritariamente hombres jóvenes que…
Uso de blogs en Ciencias de la Actividad Física y el Deporte
2011
Se describe la experiencia de un proyecto de innovación educativa que consistió en el diseño, desarrollo y evaluación de diversos usos de blogs y que se desarrolló en la Universitat de València, durante el curso académico 2009-10. El análisis refleja la heterogeneidad con la que los diferentes profesores dieron respuesta a los problemas específicos planteados en sus materias. La principal conclusión es que esta flexibilidad en el uso de los blogs supone una singular ventaja para la innovación en educación superior centrada en el aprendizaje de los estudiantes.
Responsabilidad deportiva de los atletas profesionales: esquiadores y jugadores de futbol a la luz del Derecho vigente
2018
Deporte y responsabilidad es un binomio, que más allá de las apariencias, no es contradictorio: el primero, lúdico y recreativo; el segundo referido a la violación de reglas y preceptos que pueden producir efectos en el plano procesal y/o resarcitorio. De importancia central en la responsabilidad deportiva es el común denominador en las diversas clasificaciones del “riesgo deportivo”, que se incluye en la acepción más amplia del “riesgo consentido” para aquellas actividades consideradas socialmente útiles. Esto representa una causa de justificación no codificada. El recurso al criterio de la culpa en la verificación de los ilícitos es común en la experiencia norteamericana en la que se atri…