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showing 10 items of 15058 documents

Arginase 1 Is a Marker of Myeloid-Mediated Immunosuppression with Prognostic Meaning in Classic Hodgkin Lymphoma

2016

Abstract Purpose : Neutrophilia is hallmark of classic Hodgkin Lymphoma (cHL), but its precise characterization remains elusive. We aimed at investigating the immunosuppressive role of high-density neutrophils in HL. Experimental design : First, N-HL function was evaluated in vitro, showing increased arginase (Arg-1) expression and activity compared to healthy subjects. Second, we measured serum level of Arg-1 (s-Arg-1) by ELISA in two independent, training (N=40) and validation (N=78) sets. Results : s-Arg-1 was higher in patients with advanced stage (p=0.045), B-symptoms (p=0.0048) and a positive FDG-PET scan after two cycles of chemotherapy (PET-2, p=0.012). Baseline levels of s-Arg-1 &g…

0301 basic medicineOncologymedicine.medical_specialtyPrognostic variableMyeloidmedicine.medical_treatmentImmunologyBiochemistry03 medical and health sciencesInternal medicinemedicineProgression-free survivalChemotherapybusiness.industryImmunosuppressionCell BiologyHematologymedicine.diseaseChemotherapy regimenNeutrophiliaLeukemia030104 developmental biologymedicine.anatomical_structureImmunologymedicine.symptombusinessBlood
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Predictive and Prognostic Molecular Factors in Diffuse Large B-Cell Lymphomas.

2021

Diffuse large B-cell lymphoma (DLBCL) is the commonest form of lymphoid malignancy, with a prevalence of about 40% worldwide. Its classification encompasses a common form, also termed as “not otherwise specified” (NOS), and a series of variants, which are rare and at least in part related to viral agents. Over the last two decades, DLBCL-NOS, which accounts for more than 80% of the neoplasms included in the DLBCL chapter, has been the object of an increasing number of molecular studies which have led to the identification of prognostic/predictive factors that are increasingly entering daily practice. In this review, the main achievements obtained by gene expression profiling (with respect t…

0301 basic medicineOncologymedicine.medical_specialtydiagnosisdiffuse large B-cell lymphomaReviewSettore MED/08 - Anatomia Patologica03 medical and health sciences0302 clinical medicineInternal medicineDaily practicemedicineTumor MicroenvironmentHumanslcsh:QH301-705.5B celltherapybusiness.industryGene Expression ProfilingNot Otherwise SpecifiedHigh-Throughput Nucleotide SequencingGeneral Medicinemedicine.diseaseMicroarray AnalysisPrognosisLymphomaGene expression profilingdiagnosi030104 developmental biologymedicine.anatomical_structurelcsh:Biology (General)Lymphoid malignancyclassification030220 oncology & carcinogenesisnext-generation sequencingLymphoma Large B-Cell DiffusebusinessDiffuse large B-cell lymphomaprognosiCells
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Triple negative breast cancer subtypes and pathologic complete response rate to neoadjuvant chemotherapy.

2018

Triple negative breast cancer (TNBC) is a heterogeneous disease with distinct molecular subtypes that differentially respond to chemotherapy and targeted agents. The purpose of this study is to explore the clinical relevance of Lehmann TNBC subtypes by identifying any differences in response to neoadjuvant chemotherapy among them. We determined Lehmann subtypes by gene expression profiling in paraffined pre-treatment tumor biopsies from 125 TNBC patients treated with neoadjuvant anthracyclines and/or taxanes +/- carboplatin. We explored the clinicopathological characteristics of Lehmann subtypes and their association with the pathologic complete response (pCR) to different treatments. The g…

0301 basic medicineOncologymedicine.medical_specialtysubtypingmedicine.medical_treatmentCarboplatin03 medical and health scienceschemistry.chemical_compoundpathologic complete responseSubtyping0302 clinical medicineInternal medicinePathologic complete responsemedicineClinical significanceTriple negative breast cancerneoadjuvant therapyReceptorTriple-negative breast cancerNeoadjuvant therapyChemotherapybusiness.industryPhenotypeCarboplatinGene expression profiling030104 developmental biologyOncologychemistry030220 oncology & carcinogenesistriple negative breast cancercarboplatinNeoadjuvant therapybusinessResearch Paper
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A two-gene epigenetic signature for the prediction of response to neoadjuvant chemotherapy in triple-negative breast cancer patients

2019

Background Pathological complete response (pCR) after neoadjuvant chemotherapy (NAC) in triple-negative breast cancer (TNBC) varies between 30 and 40% approximately. To provide further insight into the prediction of pCR, we evaluated the role of an epigenetic methylation-based signature. Methods Epigenetic assessment of DNA extracted from biopsy archived samples previous to NAC from TNBC patients was performed. Patients included were categorized according to previous response to NAC in responder (pCR or residual cancer burden, RCB = 0) or non-responder (non-pCR or RCB > 0) patients. A methyloma study was performed in a discovery cohort by the Infinium HumanMethylation450 BeadChip (450K arra…

0301 basic medicineOncologymedicine.medical_treatmentADNlcsh:MedicineTriple Negative Breast NeoplasmsEpigenesis Genetic0302 clinical medicineGenetics (clinical)Triple-negative breast cancermedicine.diagnostic_testHigh-Throughput Nucleotide SequencingNuclear ProteinsMethylationMiddle AgedNeoadjuvant TherapyGene Expression Regulation NeoplasticTreatment OutcomeMyogenic Regulatory FactorsEfectes secundaris dels medicaments030220 oncology & carcinogenesisCohortFemaleTaxoidsMetilacióMicrotubule-Associated ProteinsAdultmedicine.medical_specialtylcsh:QH426-470MethylationMinor Histocompatibility Antigens03 medical and health sciencesBreast cancerTriple-negative breast cancerInternal medicineCell Line TumorBiopsyGeneticsmedicineHumansEpigeneticsMolecular BiologyEpigenetic signatureAgedChemotherapybusiness.industryGene Expression ProfilingResearchlcsh:RSequence Analysis DNADNADNA Methylationmedicine.diseaseHuman geneticsRepressor Proteinslcsh:Genetics030104 developmental biologyDrug side effectsbusinessPredictionDevelopmental Biology
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Comparative Evaluation of role of Lysyl oxidase gene (LOXG473A) expression in pathogenesis and malignant transformation of Oral Submucous Fibrosis.

2019

Background Identification and comparison of gene expression of Lysyl oxidase (LOX) in oral submucous fibrosis and controls and to determine its role in Pathogenesis of Oral submucous fibrosis. Material and Methods Of total sample size (n=127), the whole blood sample were collected from case and control group in citrate vial. It is centrifused and stored at -800C. We collected and isolated RNA from blood of case group (n=127) and age and sex matched control group (n=127) recruited on the basis of inclusion criteria. The cDNA was prepared for 127 samples which were processed for gene expression of Lysyl oxidase (LOX) in relation to housekeeping genes (Beta actin and 18srRNA) and its role in p…

0301 basic medicineOral Medicine and Pathologyintegumentary systemResearchLysyl oxidaseBiologymedicine.disease:CIENCIAS MÉDICAS [UNESCO]Molecular biologyMalignant transformationHousekeeping genePathogenesis03 medical and health sciencesenzymes and coenzymes (carbohydrates)030104 developmental biology0302 clinical medicineOral submucous fibrosis030220 oncology & carcinogenesisUNESCO::CIENCIAS MÉDICASGene expressionmedicineBeta-actinGeneral DentistryGeneJournal of clinical and experimental dentistry
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How to make a tongue: Cellular and molecular regulation of muscle and connective tissue formation during mammalian tongue development

2018

IF 6.614 (2016); International audience; The vertebrate tongue is a complex muscular organ situated in the oral cavity and involved in multiple functions including mastication, taste sensation, articulation and the maintenance of oral health. Although the gross embryological contributions to tongue formation have been known for many years, it is only relatively recently that the molecular pathways regulating these processes have begun to be discovered. In particular, there is now evidence that the Hedgehog, TGF-Beta, Wnt and Notch signaling pathways all play an important role in mediating appropriate signaling interactions between the epithelial, cranial neural crest and mesodermal cell pop…

0301 basic medicineOrganogenesisHedgehog signaling[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyBifid tongueMesodermWnt03 medical and health sciences0302 clinical medicineCranial neural crestTongueTongueMacroglossiamedicineAnimalsHumansTGF-betaHedgehogMammalsAglossiaMusclesMyogenesisGene Expression Regulation DevelopmentalCell BiologyAnatomymedicine.disease030104 developmental biologymedicine.anatomical_structureCranial neural crestConnective TissueNeural CrestEmbryologyGross anatomymedicine.symptom030217 neurology & neurosurgerySignal TransductionDevelopmental BiologySeminars in Cell & Developmental Biology
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Developmental transcriptomics in Atlantic haddock: Illuminating pattern formation and organogenesis in non-model vertebrates.

2016

Gadiforms such as Atlantic haddock comprise some of the world's most economically important fisheries. Understanding the early life history of these fish is a prerequisite for predicting effects of a changing environment and increased human activities. Robust assessment of the effects of environmental impacts on the embryos of non-model vertebrates is hampered by a lack of molecular resources and detailed knowledge regarding the regulation of genes and pathways in early development. Here we used mRNA sequencing to link transcriptional changes to developmental processes in haddock, specifically, pattern formation and organogenesis. Temporal expression of key developmental genes was tightly a…

0301 basic medicineOrganogenesisved/biology.organism_classification_rank.speciesOrganogenesisBioinformaticsEyeCardiovascular SystemBone and BonesTranscriptome03 medical and health sciences0302 clinical medicinebiology.animalAnimalsRNA MessengerModel organismMolecular BiologyBody PatterningGene Librarybiologyved/biologySequence Analysis RNAGene Expression ProfilingSkullFishesVertebrateComputational BiologyGene Expression Regulation DevelopmentalCell BiologyHaddockBlastulaBlastulabiology.organism_classificationGene expression profiling030104 developmental biologyMRNA SequencingEvolutionary biologyLarvaTranscriptome030217 neurology & neurosurgeryDevelopmental BiologyDevelopmental biology
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Epigenomic landscape of human colorectal cancer unveils an aberrant core of pan-cancer enhancers orchestrated by YAP/TAZ

2021

Cancer is characterized by pervasive epigenetic alterations with enhancer dysfunction orchestrating the aberrant cancer transcriptional programs and transcriptional dependencies. Here, we epigenetically characterize human colorectal cancer (CRC) using de novo chromatin state discovery on a library of different patient-derived organoids. By exploring this resource, we unveil a tumor-specific deregulated enhancerome that is cancer cell-intrinsic and independent of interpatient heterogeneity. We show that the transcriptional coactivators YAP/TAZ act as key regulators of the conserved CRC gained enhancers. The same YAP/TAZ-bound enhancers display active chromatin profiles across diverse human t…

0301 basic medicineOrganoidEpigenomicsTranscription FactorGeneral Physics and AstronomyColorectal NeoplasmAdaptor Proteins Signal Transducing; Colorectal Neoplasms; Gene Expression Regulation Neoplastic; Histone Code; Humans; Models Genetic; Organoids; RNA-Seq; Single-Cell Analysis; Trans-Activators; Transcription Factors; Tumor Cells Cultured; Enhancer Elements Genetic; Epigenesis GeneticEpigenesis Genetic0302 clinical medicineModelsAdaptor Proteins Signal Transducing Colorectal Neoplasms Gene Expression Regulation NeoplasticHistone Code Humans Models Genetic Organoids RNA-Seq Single-Cell Analysis Trans-Activators Transcription Factors Tumor Cells Cultured Enhancer Elements Genetic Epigenesis GeneticTumor Cells CulturedCancer genomicsHistone codeRNA-SeqEpigenomicsAdaptor Proteins Signal Transducing; Colorectal Neoplasms; Gene Expression Regulation Neoplastic; Histone Code; Humans; Models Genetic; Organoids; RNA-Seq; Single-Cell Analysis; Trans-Activators; Transcription Factors; Transcriptional Coactivator with PDZ-Binding Motif Proteins; Tumor Cells Cultured; YAP-Signaling Proteins; Enhancer Elements Genetic; Epigenesis GeneticMultidisciplinaryCulturedQAdaptor Proteins3. Good healthChromatinTumor CellsGene Expression Regulation NeoplasticHistone CodeOrganoidsSingle-Cell AnalysiEnhancer Elements GeneticTrans-Activator030220 oncology & carcinogenesisSingle-Cell AnalysisColorectal NeoplasmsHumanEnhancer ElementsScienceTumour heterogeneityBiologyGeneral Biochemistry Genetics and Molecular BiologyArticle03 medical and health sciencesGeneticmedicineHumansEpigeneticsEnhancerTranscription factorAdaptor Proteins Signal TransducingNeoplasticModels GeneticSignal TransducingCancerYAP-Signaling ProteinsGeneral Chemistrymedicine.diseaseColorectal cancerdigestive system diseases030104 developmental biologyGene Expression RegulationTranscriptional Coactivator with PDZ-Binding Motif ProteinsCancer cellCancer researchTrans-ActivatorsEpigenesisTranscription Factors
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Multi-criteria framework as an innovative tradeoff approach to determine the shelf-life of high pressure-treated poultry

2016

International audience; A multi-criteria framework combining safety, hygiene and sensorial quality was developed to investigate the possibility of extending the shelf-life and/or removing lactate by applying High Hydrostatic Pressure (HHP) in a ready-to-cook (RTC) poultry product. For this purpose, Salmonella and Listeria monocytogenes were considered as safety indicators and Escherichia coli as hygienic indicator. Predictive modeling was used to determine the influence of HHP and lactate concentration on microbial growth and survival of these indicators. To that end, probabilistic assessment exposure models developed in a previous study (Lerasle, M., Guillou, S., Simonin, H., Anthoine, V.,…

0301 basic medicineOrganolepticHydrostatic pressureSodium lactateEscherichia-coliPoultrylLsteria-monocytogenesInactivationchemistry.chemical_compoundSalmonella[SDV.IDA]Life Sciences [q-bio]/Food engineeringFood scienceCookingPoultry ProductsPotassium lactateMathematics2. Zero hungerHigh hydrostatic-pressurePoultry product[ SDV.IDA ] Life Sciences [q-bio]/Food engineeringRisk-risk trade-off;Lactate;Food hygiene;Food safety;Sensorial qualitySensorial qualityRisk-risk trade-off04 agricultural and veterinary sciencesGeneral Medicine040401 food scienceMeat ProductsCured beef carpaccioTasteIn-ground beefMeat-products030106 microbiologyShelf lifeMicrobiologyFood safety03 medical and health sciences0404 agricultural biotechnologyChicken meatFood PreservationEscherichia coliHydrostatic PressureFood hygieneFood microbiologyAnimalsHumansExposure assessmentbusiness.industryDifferent temperaturesFood safetyListeria monocytogeneschemistryFood StorageConsumer Product SafetyLactatebusinessFood Science
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Innovative technologies for food preservation

2018

Abstract Several techniques have been developed during the 20th century in order to preserve foods. These innovative technologies vary considerably and embrace physical technologies (e.g., high hydrostatic pressure and high-pressure homogenization), electromagnetic technologies (e.g., pulsed electric fields, ohmic heating, microwaves, radio-frequency, and UV-light), acoustic technologies (e.g., ultrasound and shockwaves), and others such as membrane filtration and dense phase CO 2 . In this chapter, the theoretical background and definition of the technologies are explained together with a description of the equipment, main technological/processing parameters, and some advantages and limita…

0301 basic medicineOther Engineering and Technologies030109 nutrition & dieteticsMaterials sciencebusiness.industryHydrostatic pressureMechanical engineeringHigh-pressure homogenization04 agricultural and veterinary sciences040401 food scienceElectromagnetic03 medical and health sciences0404 agricultural biotechnologyHigh pressure homogenizationHigh hydrostatic pressureOhmic heatingUltrasoundDense phaseEngineering and TechnologyProcess engineeringbusinessPulsed electric fieldsMicrowavesMembrane filtration
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