Search results for "prime"

showing 10 items of 853 documents

Efectos de un programa de intervención (PHS) para la mejora de los hábitos saludables en la primera adolescencia

2015

Background: it is vital to monitor and promote healthy lifestyle habits in early adolescence, as it is a time of changes when future lifestyle habits are formed. Methods: a study was conducted to find out the effects of a Healthy Habits Program (HHP) in children between the ages of 10 and 12 years (N = 158). The study included an intervention group (IG) (n = 90), which participated in the HHP for 8 months, and a control group (CG) (n = 100). In order to assess healthy habits in these children we used the Inventory of Healthy Habits (IHH), the reliability of which was previously evaluated (N = 134). Results: the IHH obtained good reliability, Interclass Correlation Coefficient (range .506 - …

SchoolEarly adolescenceHealthEscuelaInterventionSaludHábitos saludablesHealthy habitsPrimera adolescenciaIntervención
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Species-specific identification of Dekkera/Brettanomyces yeasts by fluorescently labeled DNA probes targeting the 26S rRNA.

2007

Sequencing of the complete 26S rRNA genes of all Dekkera/Brettanomyces species colonizing different beverages revealed the potential for a specific primer and probe design to support diagnostic PCR approaches and FISH. By analysis of the complete 26S rRNA genes of all five currently known Dekkera/Brettanomyces species (Dekkera bruxellensis, D. anomala, Brettanomyces custersianus, B. nanus and B. naardenensis), several regions with high nucleotide sequence variability yet distinct from the D1/D2 domains were identified. FISH species-specific probes targeting the 26S rRNA gene's most variable regions were designed. Accessibility of probe targets for hybridization was facilitated by the constr…

Sequence analysisBrettanomycesMolecular Sequence DataWineBiologyApplied Microbiology and BiotechnologyMicrobiologySpecies SpecificityDNA FungalMycological Typing TechniquesIn Situ Hybridization FluorescencePhylogenyDNA PrimersGeneticsBase SequenceHybridization probeFungal geneticsNucleic acid sequenceGeneral MedicineSequence Analysis DNARibosomal RNAbiology.organism_classificationYeastNucleic Acid ProbesRNA RibosomalSaccharomycetalesNucleic Acid ConformationSpecific identificationFEMS yeast research
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First description of non-motile Yersinia ruckeri serovar I strains causing disease in rainbow trout, Oncorhynchus mykiss (Walbaum), cultured in Spain.

2006

Yersinia ruckeri, the causal agent of enteric redmouth (ERM) disease, was isolated from epizootics that occurred in different Spanish rainbow trout, Oncorhynchus mykiss (Walbaum), farms in which vaccination against ERM had been performed. In all episodes, the most pronounced clinical signs exhibited by affected fish were severe haemorrhages in the mouth, eyes and around the vent. The isolates were identified as Y. ruckeri serovar I by 16S rRNA sequencing together with serological tests. They lacked motility and lipase activity and thus belonged to biotype 2, and were highly virulent for juvenile rainbow trout, both by intraperitoneal injection (from 3.1 x 10(2) to 6.3 x 10(3) cfu per fish) …

SerotypeLipopolysaccharidesYersinia InfectionsVeterinary (miscellaneous)FisheriesVirulenceAquatic ScienceCommunicable Diseases EmergingPolymerase Chain ReactionMicrobiologyFish DiseasesAquacultureAgglutination TestsRNA Ribosomal 16SAnimalsSerologic TestsPathogenDNA PrimersbiologyVirulencebusiness.industryEnteric redmouth diseaseO Antigensbiology.organism_classificationAntibodies BacterialYersiniaTroutSpainOncorhynchus mykissRainbow troutYersinia ruckeribusinessJournal of fish diseases
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Nucleotide variation in the VP7 gene affects PCR genotyping of G9 rotaviruses identified in Italy

2003

A modified (aFT9m) and a degenerate (aFT9d) version of the rotavirus G9-specific primer (aFT9) allowed strains that were previously untypable, because of point mutations accumulating at the primer binding site, to be G typed by reverse transcription-polymerase chain reaction. The strains were collected during 2001-2002 in Italy in hospitals of the Apulia region, from children affected by severe rotavirus-associated enteritis. Using a wide selection of G9 rotaviruses detected worldwide, sequencing of the G9 untypable strains, sequence comparison, and phylogenetic analysis showed that the Italian strains have strong genetic similarity (< or =99.4%) to G9 rotaviruses identified recently in man…

SerotypeRotavirusGenotypeReassortmentMolecular Sequence DataBiologymedicine.disease_causePolymerase Chain ReactionRotavirus InfectionsVirologyRotavirusGenetic variationGenotypemedicineHumansTypingChildGenotypingAntigens ViralPhylogenyDNA PrimersGeneticsBase SequenceGenetic VariationVirologyEnteritisInfectious DiseasesItalyCapsid ProteinsPrimer binding site
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Use of TaqMan® real-time PCR for rapid detection of Salmonella enterica serovar Typhi

2014

We evaluated the performances of a newly designed real-time polymerase chain reaction (PCR) assay using TaqMan® probes to detectSalmonellaTyphi. TaqMan® real-time PCR assays were performed by designed primers and probe based on thestaGgene for detectingS.Typhi. The specificity of the assay was evaluated on 15Salmonellaserovars. The analytical specificity was evaluated on 20 non-Salmonellamicroorganisms. The analytical sensitivity was assessed using decreasing DNA quantities ofS.Typhi ATCC 19430. Finally the detection capability of the TaqMan® real-time PCR assay on isolates recovered from patients withSalmonellainfections was compared to the conventional PCR assay. OnlyS.Typhi strain had po…

SerotypeSalmonellaSettore MED/07 - Microbiologia E Microbiologia ClinicaBiologySalmonella typhimedicine.disease_causeReal-Time Polymerase Chain ReactionSettore MED/42 - Igiene Generale E ApplicataRapid detectionSensitivity and Specificitylaw.inventionlawTaqManmedicineHumansTyphoid FeverPolymerase chain reactionreal time typhoid fever diagnosisDNA PrimersGeneral Immunology and MicrobiologyGeneral MedicineSalmonella typhiVirologyMolecular biologyBacterial Typing TechniquesReal-time polymerase chain reactionSalmonella enterica serovar TyphiGenes Bacterial
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Evaluation of a modified single-enzyme amplified fragment length polymorphism (SE-AFLP) technique for subtyping Salmonella enterica serotype Enteriti…

2006

Salmonella enterica subsp. enterica serotype Enteritidis is not readily subtyped beyond the level of phage type (PT). Pulsed field gel electrophoresis (PFGE) is generally acknowledged to be the most discriminating typing method for Salmonella, but only a restricted variety of PFGE types has been described for S. enterica serotype Enteritidis. In the present study, a modification of the SE-AFLP typing method was used to investigate both outbreak and apparently sporadic isolates of S. enterica serotype Enteritidis belonging to different PTs and/or PFGE types. The method proved to be as discriminatory as PFGE when combined with phage typing, and provided subtyping data consistent with epidemio…

SerotypeSalmonellaSettore MED/07 - Microbiologia E Microbiologia ClinicaSalmonella enteritidismedicine.disease_causeSettore MED/42 - Igiene Generale E ApplicataMicrobiologySensitivity and SpecificitymedicineHumansTypingMolecular BiologyPhage typingDNA PrimersbiologyGeneral MedicineNucleic acid amplification techniquebiology.organism_classificationSalmonella typing SE-AFLPVirologySubtypingBacterial Typing TechniquesRandom Amplified Polymorphic DNA TechniqueSalmonella enteritidisSalmonella entericaSalmonella InfectionsNucleic Acid Amplification Techniques
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MOLECULAR ANALYSIS OF COLLETOTRICHUM SPECIES ASSOCIATED TO OLIVE IN CALABRIA.

2013

A new molecular approach based on the use of genus-specific primers targeting the internal transcribed spacer (ITS) regions of rDNA, was developed and used to study the diversity of Colletotrichum species associated with the olive canopy in the Gioia Tauro plain (Calabria, southern Italy). Representative symptomatic and symptomless samples of leaves, flowers and fruits were collected during 2012 and analyzed by extracting total DNA and amplifying the target region with the genus-specific primers. Amplicons were cloned and sequenced in order to use the ITS as a barcode gene. No Colletotrichum species were detected in the first sampling period (May 28, 2012), whereas around 15% of the analyze…

Settore AGR/12 - Patologia VegetaleColletotrichum spp. olive plants genus-specific primers metagenomic analyses
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Metagenomic strategies to assess genetic diversity in Phytophthora species

Settore AGR/12 - Patologia VegetaleMetabarcoding Phytophthora spp. genus specific primers Sanger/cloning sequencing pyrosequencing MiSeq Illumina
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Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Settore BIO/18 - Geneticainstead chromosomes of controls were almost uniformly decorated by brilliant grains. Studies on methylation of PTHrP gene promoter 2 performed on five CpG island internal sites using the Methylation-Sensitive Restriction Endonuclease Multiplex (MSREM)-PCR showed that one of the sites nearest the trascription starting point is heavy methylated in a significantly high number of RA patients. Thus RA seems to be characterized by genomewide hypomethylation associated with local hypermethylation like the most part of tumors. This result raises the possibility that susceptibility to lymphomas is related to abnormal DNA methylation levels and suggests the opportunity to evaluate the DNA methylation status in RA patientin fact the demethylating therapies together with diet and life style can act towards an increase of tumor risk. Future studies using a larger number of subjects could confirm these findings.Rheumatoid Arthritis (RA) is a chronic multisystem inflammatory disease characterized by high recurrence of lymphomas as well as hypercalcemia due to PTHrP overexpression. Because of DNA methylation plays a critical role in development of neoplasias we determined in RA patients the global DNA methylation status and local methylation pattern of the CpG island of one of the three promoters of PTHrP gene utilizing molecular and cytogenetic techniques. Investigations performed on DNA from peripheral blood of patients and donors amplified by Methylation-Sensitive Arbitrarily Primed (MeS-AP)-PCR indicated that RA is strongly associated with global DNA hypomethylation. Similarly chromosomal DNA methylation pattern analysis by indirect immunofluorescence technique with anti 5-methylcitosine antibody showed all peripheral lymphocyte metaphases from RA patients with chromosomes weakly fluorescent without discrete grain
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Durabilità del legno lamellare rinforzato: un prodotto innovativo

2010

La ricerca sviluppata nell’ultimo decennio in edilizia si è orientata sempre più verso quei materiali, cosiddetti ecocompatibili o ecosostenibili, tali da dissipare il meno possibile le risorse naturali. In questo senso il legno, ed in particolare quello lamellare, è divenuto una valida alternativa alle tecnologie più usuali e tradizionali del cemento armato e del ferro, anche perché rappresenta una delle poche materie prime rinnovabili. Il legno lamellare è un prodotto evoluto, un prodotto industriale che supera i difetti propri del legno massello con caratteristiche di resistenza meccanica e di durabilità superiori, così da poter ottenere qualunque forma e dimensione. Presenta però una ri…

Settore ICAR/10 - Architettura TecnicaLegno lamellare materie prime rinnovabili rinforzo strutturale
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