Search results for "printing"

Positive selection at codon 38 of the human KCNE1 (= minK) gene and sporadic absence of 38Ser-coding mRNAs in Gly38Ser heterozygotes

2009

Abstract Background KCNE1 represents the regulatory beta-subunit of the slowly activating delayed rectifier potassium channel (IKs). Variants of KCNE1 have repeatedly been linked to the long-QT syndrome (LQTS), a disorder which predisposes to deafness, ventricular tachyarrhythmia, syncope, and sudden cardiac death. Results We here analyze the evolution of the common Gly38Ser variant (rs1805127), using genomic DNAs, complementary DNAs, and HEK293-expressed variants of altogether 19 mammalian species. The between species comparison reveals that the human-specific Gly38Ser polymorphism evolved under strong positive Darwinian selection, probably in adaptation to specific challenges in the fine-…

OPUNTIA FICUS INDICA/MATER-BI® BASED GREEN COMPOSITES FOR FERTILIZER CONTROLLED RELEASE DEVICES PRODUCTION

STRUMENTI E MATERIALI PER LA FABBRICAZIONE DIGITALE IN ARCHITETTURA/INSTRUMENTS AND MATERIALS FOR DIGITAL MANUFACTURING IN ARCHITECTURE

2017

Il contributo descrive strumenti e materiali oggi disponibili per la fabbricazione digitale in architettura, offrendo un’ampia panoramica sulle tecniche di stampa 3D e su come potrebbe rivoluzionare le costruzioni con importanti ricadute tecniche, economiche e sociali. Dal concetto di mass customization alla creazione dei Fab Lab, dal Contour Crafting alla tecnologia D- Shape e al progetto WASP assistiamo a un ripensamento del processo architettonico, ma anche a una ricerca sui materiali naturali, ecosostenibili e “programmabili”. The article describes tools and materials available today for digital manufacturing in architecture, offering a wide overview of 3D printing techniques and how it…

Configurable low-cost plotter device for fabrication of multi-color sub-cellular scale microarrays.

2014

We report on the construction and operation of a low-cost plotter for fabrication of microarrays for multiplexed single-cell analyses. The printing head consists of polymeric pyramidal pens mounted on a rotation stage installed on an aluminium frame. This construction enables printing of microarrays onto glass substrates mounted on a tilt stage, controlled by a Lab-View operated user interface. The plotter can be assembled by typical academic workshops from components of less than 15 000 Euro. The functionality of the instrument is demonstrated by printing DNA microarrays on the area of 0.5 squared centimeters using up to three different oligonucleotides. Typical feature sizes are 5 μm diam…

Fabrication of Porous Hydrogenation Catalysts by a Selective Laser Sintering 3D Printing Technique

2019

Open in a separate window Three-dimensional selective laser sintering printing was utilized to produce porous, solid objects, in which the catalytically active component, Pd/SiO2, is attached to an easily printable supporting polypropylene framework. Physical properties of the printed objects, such as porosity, were controlled by varying the printing parameters. Structural characterization of the objects was performed by helium ion microscopy, scanning electron microscopy, and X-ray tomography, and the catalytic performance of the objects was tested in the hydrogenation of styrene, cyclohexene, and phenylacetylene. The results show that the selective laser sintering process provides an alte…

Geochronological evidence for Archaean and Palaeoproterozoic polymetamorphism in the Central Zone of the Limpopo Belt, South Africa

2018

Abstract The Central Zone of Limpopo Belt is a polymetamorphic and complexly deformed Precambrian terrane whose tectonic evolution is still uncertain because details on the timing of its evolution are not well established. We report zircon ages for newly discovered rocks in the Central Zone which document three distinct high-grade tectono-metamorphic events at 3.22, 2.62 and 2.02 Ga. The youngest of these has been well established, but the older two events were so far largely based only on rare zircon overgrowth rims. Most of our samples were collected from a large enclave in the 2.61 Ga Bulai pluton and constitute a strongly deformed, brecciated high-grade assemblage of supracrustal rocks,…

Analysis of artificially degraded DNA using STRs and SNPs—results of a collaborative European (EDNAP) exercise

2005

Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories…

Utility of the polymerase chain reaction-restriction fragment length polymorphisms of the intergenic spacer region of the rDNA for characterizing Gib…

2004

Summary In the present report, a total of thirty-one isolates of Gibberella fujikuroi (Sawada) Wollenw. species complex of Fusarium (section Liseola) morphologically classified as F. moniliforme according to the taxonomy of Nelson, Toussoun and Marasas (1983) were analyzed for their ability to produce fumonisin B1 and fumonisin B2 by an optimized liquid chromatographic method. They were isolated from three hosts (Zea mays, Musa sapientum and Pinus pinea). The results indicate that M. sapientum is a preferential host for G. fujikuroi isolates with low or null capacity for producing fumonisins, while isolates from Z. mays and P. pinea are generally high fumonisin producers. The molecular char…

Characterization of Fusarium spp. isolates by PCR-RFLP analysis of the intergenic spacer region of the rRNA gene (rDNA)

2004

In the present study, 44 Fusarium spp. isolates (5 Fusarium culmorum, 7 Fusarium graminearum, 1 Fusarium cerealis, 1 Fusarium poae, 26 Fusarium oxysporum, and 4 Gibberella fujikuroi species complex) were characterized morphologically, physiologically and genetically. All except one (Dutch Collection: CBS 620.72) were isolated from different hosts grown in various Spanish localizations. Morphological characterization was made according to macroscopic and microscopic aspects. Physiological characterization was based on their ability to produce zearalenone (ZEA) and type B trichothecenes (deoxynivalenol, nivalenol and 3-acetyldeoxynivalenol). ZEA was determined by liquid chromatography and tri…

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

2019

Structural DNA nanotechnology provides a viable route for building from the bottom-up using DNA as construction material. The most common DNA nanofabrication technique is called DNA origami, and it allows high-throughput synthesis of accurate and highly versatile structures with nanometer-level precision. Here, it is shown how the spatial information of DNA origami can be transferred to metallic nanostructures by combining the bottom-up DNA origami with the conventionally used top-down lithography approaches. This allows fabrication of billions of tiny nanostructures in one step onto selected substrates. The method is demonstrated using bowtie DNA origami to create metallic bowtie-shaped an…