Search results for "probe"

Flexible drift-compensation system for precise 3D force mapping in severe drift environments

2011

The acquisition of dense 3D data sets is of great importance, but also a challenge for scanning probe microscopy (SPM). Thermal drift often induces severe distortions in the data, which usually constrains the acquisition of dense data sets to experiments under ultra-high vacuum and low-temperature conditions. Atom tracking is an elegant approach to compensate for thermal drift and to position the microscope tip with highest precision. Here, we present a flexible drift compensation system which can easily be connected to existing SPM hardware. Furthermore, we describe a 3D data acquisition and position correction protocol, which is capable of handling large and non-linear drift as typically …

Interaction between strong radiation fields and two-level atoms: A canonical transformation approach

2008

Scaling laws in the distribution of galaxies

2004

Research done during the previous century established our Standard Cosmological Model. There are many details still to be filled in, but few would seriously doubt the basic premise. Past surveys have revealed that the large-scale distribution of galaxies in the Universe is far from random: it is highly structured over a vast range of scales. To describe cosmic structures, we need to build mathematically quantifiable descriptions of structure. Identifying where scaling laws apply and the nature of those scaling laws is an important part of understanding which physical mechanisms have been responsible for the organization of clusters, superclusters of galaxies and the voids between them. Find…

Mapping of Polytene Chromosomes

2000

Principle and Polytene chromosomes consist of up to several thousands of chromatids applications and are therefore especially suitable for direct mapping with the help ofIn situhybridization. With the introduction of nonradioactive labeling and detection methods, e.g., fluorescenceIn situhybridization (FISH) (Lan-ger-Safer et al., 1982), theIn situhybridization procedure has become easy to perform and the results can be obtained within a day. Furthermore, the method described here (Schmidt et al., 1988; Schmidt, 1992) is a simplified version which additionally allows for the hybridization of more than one DNA probe simultaneously. This double or multicolor hybridization results in very prec…

Effects of carboxyamidotriazole on in vitro models of imatinib-resistant chronic myeloid leukemia.

2008

Although imatinib mesylate (IM) has revolutionized the treatment of chronic myeloid leukemia (CML), some patients develop resistance with progression of leukemia. Alternative or additional targeting of signaling pathways deregulated in bcr-abl-driven CML cells may provide a feasible option for improving clinical response and overcoming resistance. In this study, we show that carboxyamidotriazole (CAI), an orally bioavailable calcium influx and signal transduction inhibitor, is equally effective in inhibiting the proliferation and bcr-abl dependent- and independent-signaling pathways in imatinib-resistant CML cells. CAI inhibits phosphorylation of cellular proteins including STAT5 and CrkL a…

STUDIO DELLE RELAZIONI IDRICHE E RISPOSTA ECOFISIOLOGICA NEL PISTACCHIO (PISTACIA VERA L.) SOTTOPOSTO A DIVERSI REGIMI IRRIGUI

2014

Synthèse in situ de fluorophores organiques : formation de liaisons covalentes par déclenchement enzymatique et applications en biodétection

2017

Fluorescence imaging is a growing field of biology over the past decades. Intensive works mainly focused on instrumental developments and chemistry of contrast agents (probes), were already done to improve such bioanalytical technique. The main goal of this Ph. D. thesis was to explore various fluorogenic molecular platforms responsive to various (bio)chemical stimuli and capable of releasing organic fluorophores in the biological medium to analyze. This approach named "in-situsynthesis" is based on domino reactions belonging to the repertoire of "covalent chemistry", triggered by the target (bio)analyte. This kind of process should provide advanced fluorogenic probes with high signal-to-no…

Disentangling size effects and spectral inhomogeneity in carbon nanodots by ultrafast dynamical hole-burning.

2018

Carbon nanodots (CDs) are a novel family of nanomaterials exhibiting unique optical properties. In particular, their bright and tunable fluorescence redefines the paradigm of carbon as a "black" material and is considered very appealing for many applications. While the field keeps growing, understanding CDs fundamental properties and relating them to their variable structures becomes more and more critical. Two crucial problems concern the effect of size on the electronic structure of CDs, and to what extent their optical properties are influenced by structural disorder. Furthermore, it remains largely unclear whether traditional concepts borrowed from the photo-physics of semiconductor qua…

Null alleles of human complement C4. Evidence for pseudogenes at the C4A locus and for gene conversion at the C4B locus

1990

The two genes for the C4A and C4B isotypes of the fourth component of human complement are located in the MHC class III region. Previous studies have demonstrated the unusual expression of C4 genes in the form of aberrant or duplicated haplotypes. Null alleles of C4A or C4B (AQ0 or BQ0) have been defined by the absence of gene products and occur at frequencies of 0.1-0.3. However, only some C4 null alleles are due to gene deletions, the remainder were thought to be nonexpressed genes. We have analyzed the C4 gene structure of 26 individuals lacking either C4A or C4B protein. The DNA of individuals with apparently nonexpressed C4 genes was tested for the presence of C4A- and C4B-specific seq…

Superconductive and insulating inclusions for linear and non-linear conductivity equations

2015

We detect an inclusion with infinite conductivity from boundary measurements represented by the Dirichlet-to-Neumann map for the conductivity equation. We use both the enclosure method and the probe method. We use the enclosure method to prove partial results when the underlying equation is the quasilinear $p$-Laplace equation. Further, we rigorously treat the forward problem for the partial differential equation $\operatorname{div}(\sigma\lvert\nabla u\rvert^{p-2}\nabla u)=0$ where the measurable conductivity $\sigma\colon\Omega\to[0,\infty]$ is zero or infinity in large sets and $1<p<\infty$.