Search results for "pten"
showing 10 items of 211 documents
Baculoviral display of functional scFv and synthetic IgG-binding domains.
2000
Viral vectors displaying specific ligand binding moities such as scFv fragments or intact antibodies hold promise for the development of targeted gene therapy vectors. In this report we describe baculoviral vectors displaying either functional scFv fragments or the synthetic Z/ZZ IgG binding domain derived from protein A. Display on the baculovirus surface was achieved via fusion of the scFv fragment or Z/ZZ domain to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus, AcNPV. As examples of scFv fragments we have used a murine scFv specific for the hapten 2-phenyloxazolone and a human scFv specific for carcinoembryonic antigen. In pri…
Highly sensitive monoclonal antibody-based immunoassays for the analysis of fluopyram in food samples
2019
Monoclonal antibody-based techniques have become a useful analytical technology in the agro-food sector. Nowadays, residues of the recently registered fungicide fluopyram are increasingly being found in quality control programs. In the present study, novel chemical derivatives of this pesticide were prepared and specific and high-affinity monoclonal antibodies to fluopyram were raised for the first time. Moreover, immunoassays to fluopyram were developed in two alternative enzyme-linked immunosorbent assay formats, using homologous and heterologous assay conjugates, with limits of detection below 0.05 µg L−1. The optimized immunoassays were applied to the analysis of fluopyram in fortified …
Le complexe chasséen dans l’Yonne
2020
Je ne définirai pas ici le complexe chasséen, ce qui, par ailleurs, est fait avec brio dans le présent ouvrage. En 1864, sur la colline de Chassey étaient mis au jour les premiers tessons de ce qui deviendra, au milieu du xxe s., l’un des grands sites éponymes du Néolithique français. De nombreux préhistoriens se sont penchés sur Chassey parmi lesquels figurent J. Déchelette, V. G. Childe, J. Arnal, l’Abbé Joly et enfin, J.-P. Thevenot qui publie en 2005 sous peu la synthèse d’un siècle et de...
Der Schwartzen Haupten Bruder Buch [Tallinas Melngalvju brālības goda biedru grāmata]
1711
Tallinas Melngalvju brālības goda biedru grāmata Ms.1213 Der Schwartzen Haupten Bruder Buch. [Reval, 1711.26.XII - 1939.27.X]. Tinte; papīrs, pergaments (lpp.118b, 125b). [4], 263, [3] lpp.; zīm.,kaligrāfiski rakstīta titullapa. Tukšas lpp: 30, 60, 125b, 126b, 127b, 130b, 133b, 154a, 160-229, [3] lpp. beigās.; alfab. personu reģistrs 230-263.lpp. Bojājumi, papīra plīsumi. Izgriezts gabals S.261. 41 x 15,5 x 4,2 cm LUAB. Inv. nr.R5097 RPB inv.nr. 36094 Sākumlapā attēli: Krievijas impērijas ģerbonis; Tallinas Melngalvju brālības ģerbonis. Tuša, akv. Titullapas teksts rakstīts ar tinti, dekoratīvi, kā grezns, filigrāns kaligrāfijas darbs. Paginācija vēlāka, nekonsekventa. Grāmatai pievienots W…
Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development
2021
Immunochemical methods for mycotoxin analysis require antigens with well-defined structures and antibodies with outstanding binding properties. Immunoreagents for the mycotoxins alternariol and/or alternariol monomethyl ether have typically been obtained with chemically uncharacterized haptens, and antigen conjugates have most likely been prepared with mixtures of functionalized molecules. For the first time, total synthesis was performed, in the present study, to obtain two haptens with opposite linker attachment locations. The functionalized synthetic haptens were purified and deeply characterized by different spectrometric methods, allowing the preparation of bioconjugates with unequivoc…
Synthetic Haptens and Enantioselective Monoclonal Antibodies to the Cyanotoxin Anatoxin‐a
2019
Early warning systems for monitoring toxic events may benefit from the availability of monoclonal antibodies enabling the sensitive and specific detection of anatoxin‐a, a cyanotoxin involved in numerous cases of animal poisoning due to toxic algal blooms in freshwaters. Through the synthesis of three functionalized derivatives of anatoxin‐a, we have succeeded in generating the first‐ever reported immunoreagents (bioconjugates and antibodies) suitable for the development of immunoanalytical approaches aimed at rapid and onsite detection of this harmful cyanotoxin.
Concise and modular synthesis of regioisomeric haptens for the production of high-affinity and stereoselective antibodies to the strobilurin azoxystr…
2011
The immune response to regioisomeric haptens of azoxystrobin with varied derivatization sites was studied. Based on the Sonogashira and Suzuki-Miyaura couplings and following a straightforward modular design, we have synthesized four haptens with the same linker anchored through C-C bonds and located at different sites of the molecule. The most stereoselective antibodies were produced from immunogens with the spacer arm at a distal position from the β-methoxyacrylate moiety characteristic of strobilurins. Moreover, we observed that assay cross-reactivity was reliant on the functionalization site of the competitor derivative. Finally, the antibody binding site was explored using synthetic ch…
Contact Sensitizers Specifically Increase MHC Class II Expression on Murine Immature Dendritic Cells
2000
Contact sensitivity is a T-cell-mediated immune disease that can occur when low-molecular-weight chemicals penetrate the skin. In vivo topical application of chemical sensitizers results in morphological modification of Langerhans cells (LC). Moreover, within 18 h, LC increase their major histocompatibility complex (MHC) class II antigens expression and migrate to lymph nodes where they present the sensitizer to T lymphocytes. We wanted to determine if such an effect could also be observed in vitro. However, because of the high genetic diversity encountered in humans, assays were performed with dendritic cells (DC) obtained from a Balb/c mouse strain. The capacity of a strong sensitizer, DN…
Experiments on the ectoparasitic deer ked that often attacks humans; preferences for body parts, colour and temperature.
2009
AbstractThe deer ked (Lipoptena cervi) can fail in its host search. Host search fails when an individual deer ked irreversibly accepts a host unsuitable for its reproduction (e.g. a human) and drops its wings. In northern Europe, the main host of the deer ked is the moose (Alces alces). The deer ked is increasingly causing serious problems for humans (for example, causing deer ked dermatitis) and is considered a threat for the recreational use of forests. The adult deer ked flies in early and mid-autumn to search for a host. Our aims were: (i) to study whether there are ways to avoid deer ked attacks by wearing particular clothing, and (ii) to evaluate deer ked host choice. Using human targ…
Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts
2015
Cyprodinil is among the most common agrochemical residues found in highly perishable fruits, such as strawberries. In the present study, high-affinity monoclonal antibodies to this anilinopyrimidine fungicide were raised for the first time with the aim to produce valuable immunochemical analytical assays. Cyprodinil bioconjugates and the generated novel monoclonal antibodies were employed for sensitive competitive immunoassay development in two different formats. The limits of detection of the optimized assays were 20 and 30 ng L-1 for the indirect and direct assay, respectively. Influence over assay parameters of different physicochemical factors was studied. Strawberry samples were extrac…