Search results for "pyrofosfaatti"

Measurement of pyrophosphate concentration in cells by simple terpyridine-Zn(II) probe

2015

Pyrophosphate (PPi) anion is an integral part of our physiology. It partakes in the crucial energy transactions in cells and proffers energy for the harmonious functioning of the cell machinery. Discrepancies in PPi concentrations have been associated with several pathological aberrations such as chronic pyrophosphate arthropathy, calcium pyrophosphate deposition disease, cancer etc. However, there still exists a strong demand for an efficient and specific cell based assay for PPi in order to tap its importance to full potential. Terpyridine-Zn2+ complex (ZnCl2L probe) binds with PPi in 1:3 stoichiometry. This association engenders an emission band (λem) at ~591 nm when excited at 440 nm wi…