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Evaluation of five automated and one manual method for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid.

2018

International audience; Objectives - Molecular detection of Toxoplasma gondii plays a crucial role in the prenatal and neonatal diagnosis of congenital toxoplasmosis (CT). Sensitivity of this diagnosis is partly related to the efficiency of parasite DNA extraction and amplification. DNA extraction methods with automated platforms have been developed. Therefore, it is essential to evaluate them in combination with adequate PCR amplification assays.Methods - In this multisite study, we investigated the suitability of two recent automated procedures for the isolation of Toxoplasma DNA from amniotic fluid (AF) (Magtration system 12GC, PSS and Freedom EVO VacS, Tecan), compared with three other …

0301 basic medicineMicrobiology (medical)[ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyAmniotic fluid030106 microbiologyToxoplasma gondiiPolymerase Chain ReactionSensitivity and SpecificityToxoplasmosis Congenitallaw.invention03 medical and health scienceschemistry.chemical_compound0302 clinical medicinelawparasitic diseasesDiagnosisTaqManHumans[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology030212 general & internal medicineDNA extractionPolymerase chain reactionChromatographyCongenital toxoplasmosisbiologyExtraction (chemistry)Toxoplasma gondiiNucleic Acid Hybridization[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologieGeneral Medicinerep529DNADNA Protozoanbiology.organism_classificationAmniotic FluidDNA extractionCongenital toxoplasmosisrap5293. Good healthInfectious DiseasesPCRchemistry[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieBiological AssayReagent Kits DiagnosticToxoplasmaDNAClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

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PEPhysical Sciences and Engineering
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