Search results for "rase"

The unique complexity of the CYP3A4 upstream region suggests a nongenetic explanation of its expression variability.

2010

The individually variable and unpredictable expression of CYP3A4 compromises therapies with 50% of contemporary drugs. Gene variants explain only a fraction of this variability.We investigated the evolution of CYP3A4 transcriptional regulation by nuclear receptors such as the xenobiotics sensors PXR and CAR.The combination of a proximal ER6 element with XREM and CLEM represents the original scheme of CYP3A regulation by nuclear receptors in placental mammals. Among human CYP3A genes, this scheme is retained only in CYP3A4, whereas non-CYP3A4 genes lost these elements to a variable extent during primate evolution. In parallel, the number of elements outside XREM and CLEM potentially responsi…

Hamster Bcl-2 Protein Is Cleaved in Vitro and in Cells by Caspase-9 and Caspase-3

2001

Full-length cDNA of hamster bcl-2 (771 nt) was cloned by RT-PCR and inserted into pGEX-4T-1 to produce the recombinant hamster Bcl-2 protein. The purified recombinant Bcl-2 protein (26.4 kDa) was used as a substrate for the active human caspase-3 and caspase-9 in vitro. It is shown here that Bcl-2 is efficiently cleaved by caspase-3 to a 23 kDa fragment. Although not possessing a putative caspase-9 cleavage site in its sequence, hamster Bcl-2 was also cleaved by caspase-9 into exactly the same 23 kDa cleavage product, indicating that cleavage occurred at the same site. Caspase-3- and caspase-9-mediated cleavage of Bcl-2 was efficiently blocked by caspase-3 (zDEVD) and caspase-9 (zLEHD) inhi…

Role of interleukin-6 and soluble IL-6 receptor in region-specific induction of astrocytic differentiation and neurotrophin expression.

1999

Increasing evidence supports an essential role for interleukin-6 (IL-6) in the development, differentiation, as well as de- and re-generation of neurons in the central nervous system (CNS). Both IL-6 and its specific receptor (IL-6R) are expressed on neurons and glial cells including astrocytes. In this study, we have analyzed the responses of primary rat astrocytes of various brain regions to IL-6 with respect to morphological changes and neurotrophin expression. Since IL-6 alone failed to initiate effects on astrocytes, we have examined whether the soluble IL-6R (sIL-6R) can modulate the responsiveness of to IL-6 in these cells. For this purpose, we used a highly active fusion protein of …

Purification of a biologically active recombinant glyceraldehyde 3-phosphate dehydrogenase fromCandida albicans

1999

We report here the purification of a functionally active recombinant glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Candida albicans. The GAPDH protein encoded by the TDH1 gene was obtained as a glutathione S-transferase fusion protein by expression in the vector pGEX-4T-3, and purified by affinity chromatography and thrombin digestion. The purified protein displays GAPDH enzymatic activity (42 micromol NADH min(-1) mg(-1)) as well as the laminin and fibronectin binding activities previously described. In addition, the recombinant GAPDH is covalently modified by NAD linkage; this modification is stimulated by nitric oxide and probably involves a sulfhydryl group (cysteine) residue si…

Development of type-specific and cross-reactive serological probes for the minor capsid protein of human papillomavirus type 33.

1993

Human papillomavirus type 33 (HPV33) is associated with malignant tumors of the cervix. In an attempt to develop immunological probes for HPV33 infections, antisera against various bacterial fusion proteins carrying sequences of the minor capsid protein encoded by L2 were raised in animals. Antigenic determinants on the HPV33 L2 protein were identified by using truncated fusion proteins and were classified as type specific or cross-reactive with respect to HPV1, -8, -11, -16, and -18. Cross-reactive epitopes map to amino acids 98 to 107 or to amino acids 102 to 112 and 107 to 117, respectively, depending on the fusion protein used for immunization. Antibodies directed toward these epitopes …

Cre-lox: Target Sensitivity Matters

2019

Register affects language comprehension: ERP evidence from article omission in newspaper headlines

2011

Abstract Language processing involving syntax-discourse interface operations has been claimed to be particularly resource-consuming. In production, this additional complexity is claimed to be the source of article omission in the speech of young children and certain language-impaired speakers. In comprehension, article omission in some “special registers” (e.g., newspaper headlines) has been attributed to the trade-off between spending more processing resources and increasing processing speed. We investigated the comprehension of noun phrases (NPs) with and without articles (e.g., (a) policeman arrests (a) monk) when readers were or were not aware of reading headlines by recording electroph…

Phrase frames in English pharmaceutical discourse a corpus-driven study of intradisciplinary register variation

2015

Focusing on the exploration of intra-disciplinary register variation in the pharmaceutical domain, this corpus-driven study attempts to describe the use, composition and discourse functions of phrase frames, that is, contiguous sequences of words identical except for one (Fletcher, 2002-2007), found in samples of four English pharmaceutical text types, such as patient information leaflets, summaries of product characteristics, clinical trial protocols and chapters/sections from academic textbooks on pharmacology. The study deals with a specific sub-type of phrase frames, that is, 4-word units with a variable slot in the medial position, e.g. be * with caution, to take * medicine. The result…

Keywords and lexical bundles within English pharmaceutical discourse: A corpus-driven description

2015

Abstract Little attention has been paid so far to keywords and lexical bundles used in the English language typical of the pharmaceutical field. Conducted from a register-perspective (Biber & Conrad, 2009), this exploratory and descriptive research is intended to fill in the gap in corpus linguistics studies on phraseology and register variation within written English pharmaceutical discourse. More specifically, this empirical study presents a corpus-driven description of the use and functions of keywords (top-50 by keyness) complemented by a similar description of lexical bundles (top-50 by frequency) used across samples of patient information leaflets, summaries of product characteristics…

Is there a formula for formulaic language?

2015

AbstractThis paper focuses on detecting and measuring traces of "formulaic language". For this purpose, we test a number of computational formulae that quantify the degree to which a text type incorporates inflexible sequences of words. We assess these candidate indices using a number of reference corpora representing a wide variety of text types, both routine and creative. We adopt the concept of "phrase-frame" proposed by Fletcher (2002–2007) as a means of exploring phraseological pattern variability. To date, there have been few studies explicitly addressing this issue, with the exception of Roemer (2010). We examine ten productivity indices, including Roemer's VPR, the Herfindahl-Hirsch…