Search results for "rats"

Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.

2012

To investigate the potency of the topoisomerase II (topo II) poisons doxorubicin and etoposide to stimulate the DNA damage response (DDR), S139 phosphorylation of histone H2AX (γH2AX) was analyzed using rat cardiomyoblast cells (H9c2). Etoposide caused a dose-dependent increase in the γH2AX level as shown by Western blotting. By contrast, the doxorubicin response was bell-shaped with high doses failing to increase H2AX phosphorylation. Identical results were obtained by immunohistochemical analysis of γH2AX focus formation, comet assay-based DNA strand break analysis, and measuring the formation of the topo II-DNA cleavable complex. At low dose, doxorubicin activated ataxia telangiectasia m…

Functional study of GABA-A receptors located on noradrenergic nerve endings in the rat hippocampus following prenatal exposure to diazepam

2001

Toxicological Profile of Ultrapure 2,29,3,4,49,5,59- Heptachlorbiphenyl (PCB 180) in Adult Rats

2014

Abstract: PCB 180 is a persistent non-dioxin-like polychlorinated biphenyl (NDL-PCB) abundantly present in food and the environment. Risk characterization of NDL-PCBs is confounded by the presence of highly potent dioxin-like impurities. We used ultrapure PCB 180 to characterize its toxicity profile in a 28-day repeat dose toxicity study in young adult rats extended to cover endocrine and behavioral effects. Using a loading dose/maintenance dose regimen, groups of 5 males and 5 females were given total doses of 0, 3, 10, 30, 100, 300, 1000 or 1700 mg PCB 180/kg body weight by gavage. Dose- responses were analyzed using benchmark dose modeling based on dose and adipose tissue PCB concentrati…

Effect of cholesterol-lowering treatment with a 585 on plasma membrane function and liver electron microscope appearence in rats.

1990

Physical exercise increases adult hippocampal neurogenesis in male rats provided it is aerobic and sustained

2016

Aerobic exercise, such as running, has positive effects on brain structure and function, such as adult hippocampal neurogenesis (AHN) and learning. Whether high-intensity interval training (HIT), referring to alternating short bouts of very intense anaerobic exercise with recovery periods, or anaerobic resistance training (RT) has similar effects on AHN is unclear. In addition, individual genetic variation in the overall response to physical exercise is likely to play a part in the effects of exercise on AHN but is less well studied. Recently, we developed polygenic rat models that gain differentially for running capacity in response to aerobic treadmill training. Here, we subjected these l…

Planning, implementing and evaluating a social and communication skills course for riding instructors

2016

Social and emotional skills are very important for effective coaching. As research in this field is still very limited, the purpose of this study was to plan, implement and evaluate a course teaching social and emotional skills to riding instructors. The objective of this research project was to analyse the usefulness and feasibility of a social and communication skills course for riding instructors. The present research study is an educational action research case study approach. The course was based on Thomas Gordon’s Model of Human Relationships and included different skills such as I-Messages, Active Listening, Shifting Gears and Problem Solving Skills. In total four participants took p…

Aikuisen naisen ratsastusharrastus

2000

Sahalahden talonpoikaisten rusthollisukujen perhestrategiat 1721-1886

2006

The sense of smell also ages

2013

WOS:000324010400056 ; http://www.biofutur.com/; National audience

Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O

2001

The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca 2+ -calmodulin and on intact microtubules, but was not sensitive to actin disruption or to inhibition of protein synthesis. Resealed cells were viable for days and retained the capacity to endocytose and to proliferate. The active domains of large clostridial toxins were introduced into three different cell lines. The domains were derived from Clostridium difficile B-toxin and Clo…